Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04380 and RR42_RS04385 are separated by 137 nucleotides RR42_RS04385 and RR42_RS04390 are separated by 104 nucleotides
RR42_RS04380: RR42_RS04380 - LysR family transcriptional regulator, at 974,587 to 975,540
_RS04380
RR42_RS04385: RR42_RS04385 - amino acid dehydrogenase, at 975,678 to 976,940
_RS04385
RR42_RS04390: RR42_RS04390 - ABC transporter, at 977,045 to 977,965
_RS04390
Position (kb)
975
976
977 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 974.861 kb on + strand, within RR42_RS04380 at 974.862 kb on - strand, within RR42_RS04380 at 974.862 kb on - strand, within RR42_RS04380 at 975.169 kb on + strand, within RR42_RS04380 at 975.169 kb on + strand, within RR42_RS04380 at 975.326 kb on + strand, within RR42_RS04380 at 975.347 kb on + strand, within RR42_RS04380 at 975.591 kb on - strand at 975.591 kb on - strand at 975.602 kb on + strand at 975.602 kb on + strand at 975.603 kb on - strand at 975.634 kb on + strand at 975.822 kb on + strand, within RR42_RS04385 at 975.822 kb on + strand, within RR42_RS04385 at 975.823 kb on - strand, within RR42_RS04385 at 975.823 kb on - strand, within RR42_RS04385 at 975.823 kb on - strand, within RR42_RS04385 at 975.823 kb on - strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.824 kb on + strand, within RR42_RS04385 at 975.825 kb on - strand, within RR42_RS04385 at 975.825 kb on - strand, within RR42_RS04385 at 975.825 kb on - strand, within RR42_RS04385 at 975.825 kb on - strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.828 kb on + strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.829 kb on - strand, within RR42_RS04385 at 975.868 kb on + strand, within RR42_RS04385 at 976.144 kb on - strand, within RR42_RS04385 at 976.394 kb on + strand at 976.394 kb on + strand, within RR42_RS04385 at 976.395 kb on - strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.506 kb on + strand, within RR42_RS04385 at 976.507 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.585 kb on - strand, within RR42_RS04385 at 976.977 kb on - strand at 977.011 kb on - strand at 977.044 kb on + strand at 977.152 kb on + strand, within RR42_RS04390 at 977.152 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.222 kb on + strand, within RR42_RS04390 at 977.223 kb on - strand, within RR42_RS04390 at 977.224 kb on + strand, within RR42_RS04390 at 977.250 kb on - strand, within RR42_RS04390 at 977.317 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.684 kb on + strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.757 kb on - strand, within RR42_RS04390 at 977.852 kb on + strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.853 kb on - strand, within RR42_RS04390 at 977.903 kb on + strand at 977.933 kb on + strand at 977.933 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 974,861 + RR42_RS04380 0.29 +0.0 974,862 - RR42_RS04380 0.29 +1.1 974,862 - RR42_RS04380 0.29 +1.1 975,169 + RR42_RS04380 0.61 -0.2 975,169 + RR42_RS04380 0.61 -0.5 975,326 + RR42_RS04380 0.77 -0.4 975,347 + RR42_RS04380 0.80 -0.7 975,591 - +1.0 975,591 - +0.1 975,602 + +0.1 975,602 + +0.4 975,603 - +1.1 975,634 + -0.3 975,822 + RR42_RS04385 0.11 -0.1 975,822 + RR42_RS04385 0.11 -0.1 975,823 - RR42_RS04385 0.11 -0.7 975,823 - RR42_RS04385 0.11 -1.2 975,823 - RR42_RS04385 0.11 +0.9 975,823 - RR42_RS04385 0.11 +0.1 975,824 + RR42_RS04385 0.12 -0.3 975,824 + RR42_RS04385 0.12 +0.3 975,824 + RR42_RS04385 0.12 -0.1 975,824 + RR42_RS04385 0.12 -0.4 975,824 + RR42_RS04385 0.12 +0.2 975,824 + RR42_RS04385 0.12 -0.7 975,825 - RR42_RS04385 0.12 +1.3 975,825 - RR42_RS04385 0.12 +0.9 975,825 - RR42_RS04385 0.12 -0.5 975,825 - RR42_RS04385 0.12 -2.6 975,828 + RR42_RS04385 0.12 +0.5 975,828 + RR42_RS04385 0.12 -0.2 975,828 + RR42_RS04385 0.12 -0.0 975,828 + RR42_RS04385 0.12 -0.2 975,828 + RR42_RS04385 0.12 -0.2 975,828 + RR42_RS04385 0.12 -0.1 975,828 + RR42_RS04385 0.12 +0.4 975,828 + RR42_RS04385 0.12 +0.5 975,828 + RR42_RS04385 0.12 +0.1 975,828 + RR42_RS04385 0.12 -0.4 975,828 + RR42_RS04385 0.12 +0.8 975,828 + RR42_RS04385 0.12 +0.2 975,829 - RR42_RS04385 0.12 +0.2 975,829 - RR42_RS04385 0.12 -0.3 975,829 - RR42_RS04385 0.12 -0.2 975,829 - RR42_RS04385 0.12 +1.2 975,829 - RR42_RS04385 0.12 -1.6 975,829 - RR42_RS04385 0.12 -0.6 975,829 - RR42_RS04385 0.12 -1.0 975,829 - RR42_RS04385 0.12 -0.2 975,829 - RR42_RS04385 0.12 -0.2 975,829 - RR42_RS04385 0.12 -0.2 975,868 + RR42_RS04385 0.15 -0.8 976,144 - RR42_RS04385 0.37 -0.2 976,394 + +3.2 976,394 + RR42_RS04385 0.57 -1.9 976,395 - RR42_RS04385 0.57 +0.9 976,506 + RR42_RS04385 0.66 +0.0 976,506 + RR42_RS04385 0.66 +1.2 976,507 - RR42_RS04385 0.66 -0.2 976,585 - RR42_RS04385 0.72 -0.1 976,585 - RR42_RS04385 0.72 -0.6 976,977 - +1.0 977,011 - -0.2 977,044 + +0.8 977,152 + RR42_RS04390 0.12 +0.4 977,152 + RR42_RS04390 0.12 +1.1 977,222 + RR42_RS04390 0.19 +0.5 977,222 + RR42_RS04390 0.19 -0.1 977,223 - RR42_RS04390 0.19 +0.1 977,224 + RR42_RS04390 0.19 +0.0 977,250 - RR42_RS04390 0.22 +0.1 977,317 + RR42_RS04390 0.30 +0.1 977,684 + RR42_RS04390 0.69 +1.3 977,684 + RR42_RS04390 0.69 -0.3 977,757 - RR42_RS04390 0.77 -0.3 977,757 - RR42_RS04390 0.77 +0.5 977,757 - RR42_RS04390 0.77 +0.4 977,852 + RR42_RS04390 0.88 +0.3 977,853 - RR42_RS04390 0.88 -0.9 977,853 - RR42_RS04390 0.88 -0.3 977,903 + +0.4 977,933 + +2.2 977,933 + -0.5
Or see this region's nucleotide sequence