Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03810 and RR42_RS03815 are separated by 276 nucleotides RR42_RS03815 and RR42_RS03820 are separated by 125 nucleotides RR42_RS03820 and RR42_RS03825 are separated by 110 nucleotides
RR42_RS03810: RR42_RS03810 - 3-hydroxyalkanoate synthetase, at 840,884 to 843,061
_RS03810
RR42_RS03815: RR42_RS03815 - short-chain dehydrogenase, at 843,338 to 844,117
_RS03815
RR42_RS03820: RR42_RS03820 - molybdenum cofactor biosynthesis protein MoaA, at 844,243 to 844,485
_RS03820
RR42_RS03825: RR42_RS03825 - ModE family transcriptional regulator, at 844,596 to 845,399
_RS03825
Position (kb)
843
844
845 Strain fitness (log2 ratio)
-2
-1
0
1 at 842.583 kb on - strand, within RR42_RS03810 at 842.723 kb on - strand, within RR42_RS03810 at 843.061 kb on + strand at 843.293 kb on + strand at 843.348 kb on + strand at 843.349 kb on - strand at 843.468 kb on - strand, within RR42_RS03815 at 843.485 kb on + strand, within RR42_RS03815 at 843.786 kb on - strand, within RR42_RS03815 at 843.815 kb on + strand, within RR42_RS03815 at 843.864 kb on - strand, within RR42_RS03815 at 844.040 kb on + strand at 844.085 kb on + strand at 844.085 kb on + strand at 844.086 kb on - strand at 844.174 kb on - strand at 844.183 kb on + strand at 844.183 kb on + strand at 844.184 kb on - strand at 844.229 kb on - strand at 844.313 kb on - strand, within RR42_RS03820 at 844.313 kb on - strand, within RR42_RS03820 at 844.387 kb on - strand, within RR42_RS03820 at 844.462 kb on + strand at 844.463 kb on - strand at 844.463 kb on - strand at 844.463 kb on - strand at 844.463 kb on - strand at 844.463 kb on - strand at 844.540 kb on - strand at 844.540 kb on - strand at 844.545 kb on - strand at 844.545 kb on - strand at 844.600 kb on + strand at 844.601 kb on - strand at 844.601 kb on - strand at 844.732 kb on - strand, within RR42_RS03825 at 844.864 kb on - strand, within RR42_RS03825 at 845.078 kb on - strand, within RR42_RS03825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 842,583 - RR42_RS03810 0.78 -0.8 842,723 - RR42_RS03810 0.84 -1.1 843,061 + -0.6 843,293 + +0.0 843,348 + -0.1 843,349 - -0.3 843,468 - RR42_RS03815 0.17 -0.4 843,485 + RR42_RS03815 0.19 -0.3 843,786 - RR42_RS03815 0.57 -1.4 843,815 + RR42_RS03815 0.61 +0.3 843,864 - RR42_RS03815 0.67 +0.4 844,040 + -0.5 844,085 + -0.8 844,085 + -0.6 844,086 - -0.3 844,174 - +0.3 844,183 + -0.5 844,183 + +0.1 844,184 - -1.0 844,229 - +1.5 844,313 - RR42_RS03820 0.29 -0.2 844,313 - RR42_RS03820 0.29 -0.9 844,387 - RR42_RS03820 0.59 -0.8 844,462 + -0.1 844,463 - -2.1 844,463 - -0.5 844,463 - -0.1 844,463 - -0.2 844,463 - +0.6 844,540 - -0.1 844,540 - -0.1 844,545 - -0.2 844,545 - -0.2 844,600 + -0.6 844,601 - -1.1 844,601 - -0.1 844,732 - RR42_RS03825 0.17 +0.0 844,864 - RR42_RS03825 0.33 -0.5 845,078 - RR42_RS03825 0.60 -0.7
Or see this region's nucleotide sequence