Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03435 and RR42_RS03440 are separated by 27 nucleotides RR42_RS03440 and RR42_RS03445 are separated by 179 nucleotides RR42_RS03445 and RR42_RS03450 are separated by 360 nucleotides
RR42_RS03435: RR42_RS03435 - XRE family transcriptional regulator, at 763,992 to 764,801
_RS03435
RR42_RS03440: RR42_RS03440 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 764,829 to 765,587
_RS03440
RR42_RS03445: RR42_RS03445 - adenylate kinase, at 765,767 to 766,432
_RS03445
RR42_RS03450: RR42_RS03450 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 766,793 to 767,602
_RS03450
Position (kb) 765
766
767 Strain fitness (log2 ratio) -2
-1
0
1
2 at 764.831 kb on - strand at 765.115 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.563 kb on + strand at 765.563 kb on + strand at 765.564 kb on - strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.641 kb on - strand at 765.769 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.671 kb on - strand at 766.671 kb on - strand at 766.671 kb on - strand at 766.684 kb on - strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.740 kb on - strand at 766.740 kb on - strand at 766.747 kb on - strand at 766.747 kb on - strand at 766.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 764,831 - -0.4 765,115 + RR42_RS03440 0.38 +0.0 765,161 + RR42_RS03440 0.44 -0.5 765,161 + RR42_RS03440 0.44 -0.4 765,161 + RR42_RS03440 0.44 -0.5 765,162 - RR42_RS03440 0.44 -1.4 765,162 - RR42_RS03440 0.44 -0.9 765,162 - RR42_RS03440 0.44 +0.0 765,162 - RR42_RS03440 0.44 -0.3 765,162 - RR42_RS03440 0.44 -0.0 765,162 - RR42_RS03440 0.44 -0.7 765,162 - RR42_RS03440 0.44 -0.6 765,563 + -0.5 765,563 + -0.5 765,564 - -0.7 765,567 + -0.1 765,567 + -0.3 765,567 + +0.4 765,567 + -0.3 765,567 + -0.2 765,567 + +0.9 765,567 + -0.5 765,567 + +0.1 765,567 + +0.4 765,568 - -1.4 765,568 - -0.8 765,568 - -0.9 765,568 - +0.2 765,568 - +0.4 765,568 - -2.3 765,641 - +0.4 765,769 - -2.1 766,461 - -1.1 766,461 - -0.1 766,461 - +1.0 766,461 - +0.4 766,461 - +0.8 766,461 - +2.3 766,461 - -0.7 766,461 - -0.4 766,671 - +0.6 766,671 - +0.0 766,671 - -1.2 766,684 - -0.7 766,739 + -1.5 766,739 + -0.3 766,739 + +1.1 766,739 + -0.8 766,740 - -0.6 766,740 - +0.2 766,747 - -0.7 766,747 - -0.1 766,753 + -0.8
Or see this region's nucleotide sequence