Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03060 and RR42_RS03065 are separated by 264 nucleotides RR42_RS03065 and RR42_RS03070 overlap by 4 nucleotides
RR42_RS03060: RR42_RS03060 - MFS transporter, at 676,450 to 678,150
_RS03060
RR42_RS03065: RR42_RS03065 - membrane protein, at 678,415 to 678,732
_RS03065
RR42_RS03070: RR42_RS03070 - histidine kinase, at 678,729 to 680,285
_RS03070
Position (kb) 678
679 Strain fitness (log2 ratio) -2
-1
0
1 at 677.507 kb on + strand, within RR42_RS03060 at 677.508 kb on - strand, within RR42_RS03060 at 677.508 kb on - strand, within RR42_RS03060 at 677.508 kb on - strand, within RR42_RS03060 at 677.660 kb on + strand, within RR42_RS03060 at 677.660 kb on + strand, within RR42_RS03060 at 677.660 kb on + strand, within RR42_RS03060 at 677.660 kb on + strand, within RR42_RS03060 at 677.661 kb on - strand, within RR42_RS03060 at 677.661 kb on - strand, within RR42_RS03060 at 677.661 kb on - strand, within RR42_RS03060 at 677.699 kb on + strand, within RR42_RS03060 at 677.699 kb on + strand, within RR42_RS03060 at 677.700 kb on - strand, within RR42_RS03060 at 677.700 kb on - strand, within RR42_RS03060 at 677.700 kb on - strand, within RR42_RS03060 at 677.861 kb on + strand, within RR42_RS03060 at 677.862 kb on - strand, within RR42_RS03060 at 677.862 kb on - strand, within RR42_RS03060 at 677.862 kb on - strand, within RR42_RS03060 at 677.862 kb on - strand, within RR42_RS03060 at 678.264 kb on - strand at 678.298 kb on + strand at 678.313 kb on + strand at 678.313 kb on + strand at 678.313 kb on + strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.416 kb on + strand at 678.416 kb on + strand at 678.417 kb on - strand at 678.497 kb on + strand, within RR42_RS03065 at 678.497 kb on + strand, within RR42_RS03065 at 678.497 kb on + strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.498 kb on - strand, within RR42_RS03065 at 678.519 kb on - strand, within RR42_RS03065 at 678.635 kb on - strand, within RR42_RS03065 at 678.806 kb on - strand at 678.806 kb on - strand at 678.911 kb on - strand, within RR42_RS03070 at 678.979 kb on + strand, within RR42_RS03070 at 679.043 kb on - strand, within RR42_RS03070 at 679.418 kb on + strand, within RR42_RS03070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 677,507 + RR42_RS03060 0.62 +0.8 677,508 - RR42_RS03060 0.62 -0.6 677,508 - RR42_RS03060 0.62 +0.1 677,508 - RR42_RS03060 0.62 +0.1 677,660 + RR42_RS03060 0.71 +0.1 677,660 + RR42_RS03060 0.71 -0.6 677,660 + RR42_RS03060 0.71 +1.7 677,660 + RR42_RS03060 0.71 -0.7 677,661 - RR42_RS03060 0.71 -0.1 677,661 - RR42_RS03060 0.71 +0.1 677,661 - RR42_RS03060 0.71 +0.5 677,699 + RR42_RS03060 0.73 +0.1 677,699 + RR42_RS03060 0.73 +1.3 677,700 - RR42_RS03060 0.73 +0.2 677,700 - RR42_RS03060 0.73 +0.1 677,700 - RR42_RS03060 0.73 -0.9 677,861 + RR42_RS03060 0.83 +0.1 677,862 - RR42_RS03060 0.83 -2.3 677,862 - RR42_RS03060 0.83 +0.4 677,862 - RR42_RS03060 0.83 +0.4 677,862 - RR42_RS03060 0.83 +0.9 678,264 - +0.4 678,298 + +0.2 678,313 + -0.1 678,313 + +1.4 678,313 + -0.9 678,314 - +0.3 678,314 - +0.2 678,314 - -1.2 678,416 + +0.9 678,416 + -0.6 678,417 - +0.2 678,497 + RR42_RS03065 0.26 +1.4 678,497 + RR42_RS03065 0.26 +0.5 678,497 + RR42_RS03065 0.26 -1.7 678,498 - RR42_RS03065 0.26 +0.1 678,498 - RR42_RS03065 0.26 -0.5 678,498 - RR42_RS03065 0.26 +0.9 678,498 - RR42_RS03065 0.26 +1.3 678,498 - RR42_RS03065 0.26 +1.3 678,498 - RR42_RS03065 0.26 -0.3 678,519 - RR42_RS03065 0.33 +0.4 678,635 - RR42_RS03065 0.69 +0.3 678,806 - -0.1 678,806 - -2.6 678,911 - RR42_RS03070 0.12 +0.2 678,979 + RR42_RS03070 0.16 +0.1 679,043 - RR42_RS03070 0.20 +1.1 679,418 + RR42_RS03070 0.44 -0.1
Or see this region's nucleotide sequence