Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02525 and RR42_RS02530 are separated by 26 nucleotides RR42_RS02530 and RR42_RS02535 are separated by 177 nucleotides RR42_RS02535 and RR42_RS02540 are separated by 296 nucleotides
RR42_RS02525: RR42_RS02525 - enoyl-CoA hydratase, at 568,689 to 569,465
_RS02525
RR42_RS02530: RR42_RS02530 - acyl-CoA hydrolase, at 569,492 to 569,914
_RS02530
RR42_RS02535: RR42_RS02535 - metal ABC transporter permease, at 570,092 to 571,987
_RS02535
RR42_RS02540: RR42_RS02540 - taurine ABC transporter permease, at 572,284 to 573,306
_RS02540
Position (kb) 570
571
572 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 569.276 kb on - strand, within RR42_RS02525 at 569.464 kb on - strand at 569.464 kb on - strand at 569.464 kb on - strand at 569.542 kb on + strand, within RR42_RS02530 at 569.542 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.559 kb on + strand, within RR42_RS02530 at 569.560 kb on - strand, within RR42_RS02530 at 569.619 kb on + strand, within RR42_RS02530 at 569.620 kb on - strand, within RR42_RS02530 at 569.633 kb on + strand, within RR42_RS02530 at 570.042 kb on + strand at 570.046 kb on + strand at 570.046 kb on + strand at 570.071 kb on + strand at 570.091 kb on + strand at 570.101 kb on + strand at 570.102 kb on - strand at 570.106 kb on + strand at 570.106 kb on + strand at 570.107 kb on - strand at 570.107 kb on - strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.203 kb on + strand at 570.216 kb on - strand at 570.216 kb on - strand at 570.593 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.653 kb on + strand, within RR42_RS02535 at 570.659 kb on - strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.698 kb on + strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.699 kb on - strand, within RR42_RS02535 at 570.700 kb on + strand, within RR42_RS02535 at 570.701 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535 at 570.840 kb on - strand, within RR42_RS02535 at 571.050 kb on - strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.346 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.469 kb on + strand, within RR42_RS02535 at 571.473 kb on - strand, within RR42_RS02535 at 571.473 kb on - strand, within RR42_RS02535 at 571.574 kb on + strand, within RR42_RS02535 at 572.079 kb on + strand at 572.079 kb on + strand at 572.080 kb on - strand at 572.080 kb on - strand at 572.089 kb on - strand at 572.157 kb on - strand at 572.157 kb on - strand at 572.157 kb on - strand at 572.181 kb on - strand at 572.434 kb on + strand, within RR42_RS02540 at 572.435 kb on - strand, within RR42_RS02540 at 572.609 kb on - strand, within RR42_RS02540 at 572.917 kb on + strand, within RR42_RS02540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 569,276 - RR42_RS02525 0.76 -0.5 569,464 - +0.5 569,464 - +0.1 569,464 - +0.2 569,542 + RR42_RS02530 0.12 -0.0 569,542 + RR42_RS02530 0.12 -1.3 569,559 + RR42_RS02530 0.16 -0.9 569,559 + RR42_RS02530 0.16 -1.1 569,559 + RR42_RS02530 0.16 +0.5 569,559 + RR42_RS02530 0.16 -0.6 569,560 - RR42_RS02530 0.16 -0.2 569,619 + RR42_RS02530 0.30 +0.5 569,620 - RR42_RS02530 0.30 -0.1 569,633 + RR42_RS02530 0.33 +0.2 570,042 + -0.9 570,046 + -0.9 570,046 + +0.4 570,071 + -0.2 570,091 + -1.0 570,101 + +1.9 570,102 - -0.4 570,106 + -0.1 570,106 + +0.5 570,107 - -0.1 570,107 - -0.4 570,203 + +0.4 570,203 + -0.4 570,203 + +0.5 570,216 - -1.1 570,216 - -0.6 570,593 + RR42_RS02535 0.26 -0.1 570,653 + RR42_RS02535 0.30 +0.8 570,653 + RR42_RS02535 0.30 -0.5 570,653 + RR42_RS02535 0.30 -1.1 570,659 - RR42_RS02535 0.30 +0.7 570,698 + RR42_RS02535 0.32 -1.1 570,698 + RR42_RS02535 0.32 -0.4 570,698 + RR42_RS02535 0.32 +0.4 570,699 - RR42_RS02535 0.32 -0.0 570,699 - RR42_RS02535 0.32 -0.7 570,699 - RR42_RS02535 0.32 -0.5 570,699 - RR42_RS02535 0.32 +0.2 570,699 - RR42_RS02535 0.32 +0.5 570,699 - RR42_RS02535 0.32 -0.5 570,700 + RR42_RS02535 0.32 +0.7 570,701 - RR42_RS02535 0.32 -0.1 570,840 - RR42_RS02535 0.39 -1.1 570,840 - RR42_RS02535 0.39 -0.1 571,050 - RR42_RS02535 0.51 -0.4 571,346 + RR42_RS02535 0.66 -1.7 571,346 + RR42_RS02535 0.66 -0.9 571,346 + RR42_RS02535 0.66 -0.5 571,469 + RR42_RS02535 0.73 -0.6 571,469 + RR42_RS02535 0.73 -1.0 571,469 + RR42_RS02535 0.73 -0.5 571,473 - RR42_RS02535 0.73 -1.1 571,473 - RR42_RS02535 0.73 -0.2 571,574 + RR42_RS02535 0.78 -1.2 572,079 + -1.5 572,079 + +0.2 572,080 - -0.4 572,080 - +0.1 572,089 - -1.0 572,157 - +0.2 572,157 - +0.1 572,157 - -0.3 572,181 - -0.3 572,434 + RR42_RS02540 0.15 -0.3 572,435 - RR42_RS02540 0.15 +0.4 572,609 - RR42_RS02540 0.32 -3.4 572,917 + RR42_RS02540 0.62 +0.6
Or see this region's nucleotide sequence