Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02150 and RR42_RS02155 are separated by 197 nucleotides RR42_RS02155 and RR42_RS02160 are separated by 128 nucleotides RR42_RS02160 and RR42_RS02165 are separated by 102 nucleotides
RR42_RS02150: RR42_RS02150 - membrane protein, at 478,095 to 479,351
_RS02150
RR42_RS02155: RR42_RS02155 - single-stranded DNA-binding protein, at 479,549 to 480,103
_RS02155
RR42_RS02160: RR42_RS02160 - LacI family transcriptional regulator, at 480,232 to 481,215
_RS02160
RR42_RS02165: RR42_RS02165 - LysR family transcriptional regulator, at 481,318 to 482,256
_RS02165
Position (kb) 480
481
482 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 479.285 kb on + strand at 479.285 kb on + strand at 479.287 kb on + strand at 479.417 kb on + strand at 479.421 kb on + strand at 479.421 kb on + strand at 479.468 kb on + strand at 480.321 kb on + strand at 480.450 kb on - strand, within RR42_RS02160 at 480.450 kb on - strand, within RR42_RS02160 at 480.460 kb on + strand, within RR42_RS02160 at 480.460 kb on + strand, within RR42_RS02160 at 480.461 kb on - strand, within RR42_RS02160 at 480.461 kb on - strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.653 kb on + strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.654 kb on - strand, within RR42_RS02160 at 480.741 kb on - strand, within RR42_RS02160 at 480.770 kb on + strand, within RR42_RS02160 at 480.771 kb on - strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.893 kb on + strand, within RR42_RS02160 at 480.894 kb on + strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 480.942 kb on - strand, within RR42_RS02160 at 481.149 kb on + strand at 481.149 kb on + strand at 481.150 kb on - strand at 481.271 kb on + strand at 481.271 kb on + strand at 481.272 kb on - strand at 481.275 kb on + strand at 481.275 kb on + strand at 481.276 kb on - strand at 481.810 kb on - strand, within RR42_RS02165 at 481.810 kb on - strand, within RR42_RS02165 at 481.810 kb on - strand, within RR42_RS02165 at 481.829 kb on - strand, within RR42_RS02165 at 481.914 kb on - strand, within RR42_RS02165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 479,285 + -2.7 479,285 + -0.6 479,287 + -2.7 479,417 + +3.4 479,421 + +0.0 479,421 + -0.1 479,468 + +0.5 480,321 + -1.3 480,450 - RR42_RS02160 0.22 -0.2 480,450 - RR42_RS02160 0.22 +0.6 480,460 + RR42_RS02160 0.23 -0.9 480,460 + RR42_RS02160 0.23 -1.1 480,461 - RR42_RS02160 0.23 -0.5 480,461 - RR42_RS02160 0.23 +0.0 480,653 + RR42_RS02160 0.43 +0.1 480,653 + RR42_RS02160 0.43 -0.9 480,653 + RR42_RS02160 0.43 -0.8 480,654 - RR42_RS02160 0.43 -0.1 480,654 - RR42_RS02160 0.43 +0.2 480,654 - RR42_RS02160 0.43 +1.2 480,741 - RR42_RS02160 0.52 -0.4 480,770 + RR42_RS02160 0.55 -0.4 480,771 - RR42_RS02160 0.55 +0.3 480,893 + RR42_RS02160 0.67 +0.5 480,893 + RR42_RS02160 0.67 +0.0 480,893 + RR42_RS02160 0.67 -0.9 480,893 + RR42_RS02160 0.67 -0.8 480,894 + RR42_RS02160 0.67 +0.4 480,942 - RR42_RS02160 0.72 +0.0 480,942 - RR42_RS02160 0.72 -0.4 480,942 - RR42_RS02160 0.72 -0.0 480,942 - RR42_RS02160 0.72 -0.1 481,149 + +0.4 481,149 + +0.4 481,150 - +0.0 481,271 + -0.9 481,271 + -0.9 481,272 - +1.7 481,275 + -0.2 481,275 + -0.1 481,276 - +0.0 481,810 - RR42_RS02165 0.52 -0.6 481,810 - RR42_RS02165 0.52 +0.0 481,810 - RR42_RS02165 0.52 -0.4 481,829 - RR42_RS02165 0.54 +0.4 481,914 - RR42_RS02165 0.63 -0.5
Or see this region's nucleotide sequence