Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02045 and RR42_RS02050 are separated by 99 nucleotides RR42_RS02050 and RR42_RS02055 are separated by 6 nucleotides RR42_RS02055 and RR42_RS02060 are separated by 66 nucleotides
RR42_RS02045: RR42_RS02045 - GTPase, at 455,455 to 457,398
_RS02045
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
RR42_RS02055: RR42_RS02055 - ATP-dependent protease, at 458,674 to 459,351
_RS02055
RR42_RS02060: RR42_RS02060 - nucleotide-binding protein, at 459,418 to 460,335
_RS02060
Position (kb) 457
458
459 Strain fitness (log2 ratio) -2
-1
0
1
2 at 456.928 kb on + strand, within RR42_RS02045 at 456.928 kb on + strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.929 kb on - strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.957 kb on + strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.958 kb on - strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.994 kb on + strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 456.995 kb on - strand, within RR42_RS02045 at 457.059 kb on + strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.060 kb on - strand, within RR42_RS02045 at 457.107 kb on + strand, within RR42_RS02045 at 457.295 kb on + strand at 457.295 kb on + strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.296 kb on - strand at 457.298 kb on + strand at 457.298 kb on + strand at 457.299 kb on - strand at 457.492 kb on + strand at 457.642 kb on + strand, within RR42_RS02050 at 457.702 kb on + strand, within RR42_RS02050 at 457.703 kb on - strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.795 kb on + strand, within RR42_RS02050 at 457.798 kb on + strand, within RR42_RS02050 at 457.799 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.108 kb on - strand, within RR42_RS02050 at 458.463 kb on + strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.606 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.703 kb on + strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.881 kb on - strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.244 kb on + strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.249 kb on + strand, within RR42_RS02055 at 459.283 kb on - strand, within RR42_RS02055 at 459.459 kb on + strand at 459.459 kb on + strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.477 kb on - strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.501 kb on - strand at 459.501 kb on - strand at 459.549 kb on - strand, within RR42_RS02060 at 459.549 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.601 kb on + strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 456,928 + RR42_RS02045 0.76 +1.6 456,928 + RR42_RS02045 0.76 +0.6 456,929 - RR42_RS02045 0.76 +0.9 456,929 - RR42_RS02045 0.76 +1.1 456,929 - RR42_RS02045 0.76 +0.1 456,957 + RR42_RS02045 0.77 +0.1 456,957 + RR42_RS02045 0.77 +1.2 456,957 + RR42_RS02045 0.77 +0.1 456,957 + RR42_RS02045 0.77 +0.9 456,958 - RR42_RS02045 0.77 +0.5 456,958 - RR42_RS02045 0.77 -0.1 456,958 - RR42_RS02045 0.77 +1.3 456,958 - RR42_RS02045 0.77 +0.3 456,958 - RR42_RS02045 0.77 -0.7 456,994 + RR42_RS02045 0.79 +1.0 456,994 + RR42_RS02045 0.79 +1.8 456,994 + RR42_RS02045 0.79 +0.7 456,994 + RR42_RS02045 0.79 +1.5 456,995 - RR42_RS02045 0.79 +0.3 456,995 - RR42_RS02045 0.79 +0.9 457,059 + RR42_RS02045 0.83 +0.4 457,060 - RR42_RS02045 0.83 +0.9 457,060 - RR42_RS02045 0.83 +2.0 457,107 + RR42_RS02045 0.85 -0.5 457,295 + +0.6 457,295 + +0.7 457,296 - +1.1 457,296 - +1.4 457,296 - -0.7 457,298 + +0.4 457,298 + +0.6 457,299 - +0.5 457,492 + -0.7 457,642 + RR42_RS02050 0.12 -0.8 457,702 + RR42_RS02050 0.17 +0.3 457,703 - RR42_RS02050 0.18 -1.0 457,795 + RR42_RS02050 0.25 +0.4 457,795 + RR42_RS02050 0.25 +0.2 457,798 + RR42_RS02050 0.26 +0.5 457,799 - RR42_RS02050 0.26 +1.3 458,108 - RR42_RS02050 0.52 +0.6 458,108 - RR42_RS02050 0.52 +0.3 458,108 - RR42_RS02050 0.52 -0.2 458,463 + RR42_RS02050 0.82 -0.8 458,464 - RR42_RS02050 0.83 +0.3 458,464 - RR42_RS02050 0.83 +0.2 458,464 - RR42_RS02050 0.83 -0.9 458,606 + -2.3 458,699 + +0.7 458,699 + -0.3 458,699 + +1.7 458,699 + -0.3 458,699 + -0.4 458,699 + +1.4 458,699 + -0.1 458,699 + +0.3 458,699 + -0.6 458,700 - +0.3 458,700 - -0.0 458,700 - +0.6 458,700 - +0.4 458,700 - -0.5 458,700 - -1.1 458,700 - -0.1 458,703 + +0.8 458,704 - +0.0 458,704 - +0.1 458,704 - +0.6 458,704 - -2.1 458,881 - RR42_RS02055 0.31 +0.2 459,054 + RR42_RS02055 0.56 -0.2 459,054 + RR42_RS02055 0.56 -0.1 459,054 + RR42_RS02055 0.56 -0.7 459,054 + RR42_RS02055 0.56 -1.0 459,055 - RR42_RS02055 0.56 +0.8 459,055 - RR42_RS02055 0.56 -0.1 459,197 + RR42_RS02055 0.77 +0.4 459,197 + RR42_RS02055 0.77 +0.4 459,198 - RR42_RS02055 0.77 -1.0 459,198 - RR42_RS02055 0.77 +0.6 459,244 + RR42_RS02055 0.84 -1.3 459,245 - RR42_RS02055 0.84 +0.2 459,245 - RR42_RS02055 0.84 +0.0 459,245 - RR42_RS02055 0.84 -1.6 459,249 + RR42_RS02055 0.85 -0.2 459,283 - RR42_RS02055 0.90 +0.5 459,459 + +1.5 459,459 + +1.5 459,460 - +1.5 459,460 - +1.0 459,460 - +1.9 459,460 - +1.1 459,460 - +1.7 459,476 + -0.4 459,476 + +1.3 459,476 + +1.1 459,476 + +0.8 459,477 - +2.1 459,500 + +1.7 459,500 + +1.1 459,500 + +1.4 459,501 - +0.9 459,501 - +1.4 459,549 - RR42_RS02060 0.14 +1.5 459,549 - RR42_RS02060 0.14 +1.3 459,594 - RR42_RS02060 0.19 +1.8 459,594 - RR42_RS02060 0.19 +1.3 459,601 + RR42_RS02060 0.20 +1.5 459,602 - RR42_RS02060 0.20 +0.8 459,602 - RR42_RS02060 0.20 +1.3 459,602 - RR42_RS02060 0.20 +1.2 459,602 - RR42_RS02060 0.20 +0.9 459,602 - RR42_RS02060 0.20 +1.4
Or see this region's nucleotide sequence