Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01810 and RR42_RS01815 are separated by 15 nucleotides RR42_RS01815 and RR42_RS01820 are separated by 62 nucleotides RR42_RS01820 and RR42_RS01825 are separated by 161 nucleotides
RR42_RS01810: RR42_RS01810 - tRNA 2-selenouridine synthase, at 411,153 to 412,277
_RS01810
RR42_RS01815: RR42_RS01815 - molybdopterin biosynthesis protein MoeB, at 412,293 to 413,060
_RS01815
RR42_RS01820: RR42_RS01820 - peptidase S41, at 413,123 to 414,712
_RS01820
RR42_RS01825: RR42_RS01825 - phosphoglyceromutase, at 414,874 to 415,620
_RS01825
Position (kb) 413
414
415 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 412.167 kb on + strand at 412.167 kb on + strand at 412.167 kb on + strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.170 kb on + strand at 412.170 kb on + strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.632 kb on - strand, within RR42_RS01820 at 413.813 kb on - strand, within RR42_RS01820 at 413.887 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.680 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 415.664 kb on - strand at 415.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 412,167 + +0.2 412,167 + +0.1 412,167 + +0.5 412,168 - +0.5 412,168 - -0.3 412,168 - -0.6 412,168 - -0.3 412,168 - +0.1 412,170 + +0.1 412,170 + +0.1 412,171 - +0.3 412,171 - -1.2 412,171 - -0.4 412,182 + -1.3 412,182 + +0.3 412,182 + -0.5 412,182 + +0.1 412,183 - +0.8 412,183 - -1.1 412,183 - -0.2 412,183 - +1.2 412,183 - -0.3 412,183 - -0.5 412,183 - +1.3 413,626 - RR42_RS01820 0.32 -2.1 413,626 - RR42_RS01820 0.32 -0.5 413,626 - RR42_RS01820 0.32 -1.8 413,629 - RR42_RS01820 0.32 -1.4 413,629 - RR42_RS01820 0.32 -2.2 413,629 - RR42_RS01820 0.32 -3.3 413,632 - RR42_RS01820 0.32 -1.9 413,813 - RR42_RS01820 0.43 -1.3 413,887 - RR42_RS01820 0.48 -1.8 414,211 - RR42_RS01820 0.68 -2.4 414,211 - RR42_RS01820 0.68 -3.8 414,211 - RR42_RS01820 0.68 -1.0 414,550 - RR42_RS01820 0.90 +0.8 414,550 - RR42_RS01820 0.90 -3.0 414,680 - +0.4 414,793 - +1.4 414,793 - -1.3 414,793 - +0.3 414,793 - -0.5 414,793 - +0.0 414,794 + -0.9 414,794 + -2.1 414,794 + -1.4 414,794 + -0.9 414,794 + +0.1 414,794 + -0.3 414,794 + -0.4 414,794 + +0.1 414,795 - -2.8 414,795 - +0.3 414,795 - +0.9 414,795 - -0.4 414,795 - -1.6 414,795 - -0.2 414,795 - +0.0 414,795 - -0.5 415,664 - +0.9 415,685 - +0.4
Or see this region's nucleotide sequence