Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01810 and RR42_RS01815 are separated by 15 nucleotides RR42_RS01815 and RR42_RS01820 are separated by 62 nucleotides
RR42_RS01810: RR42_RS01810 - tRNA 2-selenouridine synthase, at 411,153 to 412,277
_RS01810
RR42_RS01815: RR42_RS01815 - molybdopterin biosynthesis protein MoeB, at 412,293 to 413,060
_RS01815
RR42_RS01820: RR42_RS01820 - peptidase S41, at 413,123 to 414,712
_RS01820
Position (kb) 412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 411.309 kb on + strand, within RR42_RS01810 at 411.310 kb on - strand, within RR42_RS01810 at 411.310 kb on - strand, within RR42_RS01810 at 411.432 kb on + strand, within RR42_RS01810 at 411.432 kb on + strand, within RR42_RS01810 at 411.432 kb on + strand, within RR42_RS01810 at 411.432 kb on + strand, within RR42_RS01810 at 411.433 kb on - strand, within RR42_RS01810 at 411.536 kb on + strand, within RR42_RS01810 at 411.536 kb on + strand, within RR42_RS01810 at 411.536 kb on + strand, within RR42_RS01810 at 411.537 kb on - strand, within RR42_RS01810 at 411.537 kb on - strand, within RR42_RS01810 at 411.537 kb on - strand, within RR42_RS01810 at 411.537 kb on - strand, within RR42_RS01810 at 411.812 kb on - strand, within RR42_RS01810 at 411.864 kb on + strand, within RR42_RS01810 at 411.864 kb on + strand, within RR42_RS01810 at 411.864 kb on + strand, within RR42_RS01810 at 411.864 kb on + strand, within RR42_RS01810 at 411.942 kb on + strand, within RR42_RS01810 at 411.942 kb on + strand, within RR42_RS01810 at 411.942 kb on + strand, within RR42_RS01810 at 411.942 kb on + strand, within RR42_RS01810 at 411.942 kb on + strand, within RR42_RS01810 at 411.943 kb on - strand, within RR42_RS01810 at 411.943 kb on - strand, within RR42_RS01810 at 411.943 kb on - strand, within RR42_RS01810 at 412.167 kb on + strand at 412.167 kb on + strand at 412.167 kb on + strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.168 kb on - strand at 412.170 kb on + strand at 412.170 kb on + strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.171 kb on - strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.182 kb on + strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 412.183 kb on - strand at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.626 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.629 kb on - strand, within RR42_RS01820 at 413.632 kb on - strand, within RR42_RS01820 at 413.813 kb on - strand, within RR42_RS01820 at 413.887 kb on - strand, within RR42_RS01820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 411,309 + RR42_RS01810 0.14 +0.4 411,310 - RR42_RS01810 0.14 +0.2 411,310 - RR42_RS01810 0.14 +0.0 411,432 + RR42_RS01810 0.25 -0.6 411,432 + RR42_RS01810 0.25 +0.3 411,432 + RR42_RS01810 0.25 +1.4 411,432 + RR42_RS01810 0.25 -0.2 411,433 - RR42_RS01810 0.25 -0.2 411,536 + RR42_RS01810 0.34 -0.1 411,536 + RR42_RS01810 0.34 -1.3 411,536 + RR42_RS01810 0.34 +1.9 411,537 - RR42_RS01810 0.34 -0.2 411,537 - RR42_RS01810 0.34 -0.9 411,537 - RR42_RS01810 0.34 +0.1 411,537 - RR42_RS01810 0.34 -1.0 411,812 - RR42_RS01810 0.59 -2.2 411,864 + RR42_RS01810 0.63 -0.2 411,864 + RR42_RS01810 0.63 -0.8 411,864 + RR42_RS01810 0.63 +0.7 411,864 + RR42_RS01810 0.63 -0.6 411,942 + RR42_RS01810 0.70 -0.5 411,942 + RR42_RS01810 0.70 +0.3 411,942 + RR42_RS01810 0.70 +0.3 411,942 + RR42_RS01810 0.70 -0.9 411,942 + RR42_RS01810 0.70 +0.6 411,943 - RR42_RS01810 0.70 -0.2 411,943 - RR42_RS01810 0.70 -0.8 411,943 - RR42_RS01810 0.70 +0.0 412,167 + +0.2 412,167 + +0.1 412,167 + +0.5 412,168 - +0.5 412,168 - -0.3 412,168 - -0.6 412,168 - -0.3 412,168 - +0.1 412,170 + +0.1 412,170 + +0.1 412,171 - +0.3 412,171 - -1.2 412,171 - -0.4 412,182 + -1.3 412,182 + +0.3 412,182 + -0.5 412,182 + +0.1 412,183 - +0.8 412,183 - -1.1 412,183 - -0.2 412,183 - +1.2 412,183 - -0.3 412,183 - -0.5 412,183 - +1.3 413,626 - RR42_RS01820 0.32 -2.1 413,626 - RR42_RS01820 0.32 -0.5 413,626 - RR42_RS01820 0.32 -1.8 413,629 - RR42_RS01820 0.32 -1.4 413,629 - RR42_RS01820 0.32 -2.2 413,629 - RR42_RS01820 0.32 -3.3 413,632 - RR42_RS01820 0.32 -1.9 413,813 - RR42_RS01820 0.43 -1.3 413,887 - RR42_RS01820 0.48 -1.8
Or see this region's nucleotide sequence