Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides RR42_RS00970 and RR42_RS00975 are separated by 22 nucleotides RR42_RS00975 and RR42_RS00980 are separated by 72 nucleotides RR42_RS00980 and RR42_RS00985 are separated by 58 nucleotides
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
RR42_RS00975: RR42_RS00975 - MBL fold metallo-hydrolase, at 230,222 to 231,154
_RS00975
RR42_RS00980: RR42_RS00980 - hypothetical protein, at 231,227 to 231,823
_RS00980
RR42_RS00985: RR42_RS00985 - acetyltransferase, at 231,882 to 232,496
_RS00985
Position (kb) 230
231
232 Strain fitness (log2 ratio) -2
-1
0
1
2 at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand at 229.612 kb on + strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.613 kb on - strand, within RR42_RS00970 at 229.920 kb on - strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.006 kb on + strand, within RR42_RS00970 at 230.007 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.011 kb on - strand, within RR42_RS00970 at 230.213 kb on + strand at 230.213 kb on + strand at 230.213 kb on + strand at 230.214 kb on - strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.234 kb on + strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.235 kb on - strand at 230.345 kb on + strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.547 kb on - strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.900 kb on + strand, within RR42_RS00975 at 230.901 kb on - strand, within RR42_RS00975 at 231.141 kb on - strand at 231.141 kb on - strand at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.392 kb on + strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.393 kb on - strand, within RR42_RS00980 at 231.470 kb on + strand, within RR42_RS00980 at 231.979 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.011 kb on + strand, within RR42_RS00985 at 232.013 kb on + strand, within RR42_RS00985 at 232.014 kb on - strand, within RR42_RS00985 at 232.014 kb on - strand, within RR42_RS00985 at 232.077 kb on + strand, within RR42_RS00985 at 232.078 kb on - strand, within RR42_RS00985 at 232.078 kb on - strand, within RR42_RS00985 at 232.091 kb on - strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.116 kb on + strand, within RR42_RS00985 at 232.117 kb on - strand, within RR42_RS00985 at 232.152 kb on + strand, within RR42_RS00985 at 232.153 kb on - strand, within RR42_RS00985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 229,345 + RR42_RS00965 0.43 -0.5 229,495 + -0.2 229,495 + +0.3 229,495 + -0.2 229,495 + +0.1 229,496 - -0.5 229,496 - -0.0 229,496 - -1.0 229,496 - -0.7 229,496 - +0.4 229,496 - -0.0 229,531 + +0.1 229,532 - -0.3 229,558 + -1.0 229,559 - -1.1 229,612 + RR42_RS00970 0.15 -0.5 229,613 - RR42_RS00970 0.15 -0.8 229,613 - RR42_RS00970 0.15 +0.3 229,920 - RR42_RS00970 0.59 +0.2 230,006 + RR42_RS00970 0.72 +0.9 230,006 + RR42_RS00970 0.72 -1.6 230,007 - RR42_RS00970 0.72 -0.5 230,011 - RR42_RS00970 0.73 +0.2 230,011 - RR42_RS00970 0.73 +0.8 230,011 - RR42_RS00970 0.73 -0.1 230,213 + -0.7 230,213 + -0.4 230,213 + -2.7 230,214 - -0.5 230,234 + -0.7 230,234 + +0.2 230,234 + -0.0 230,234 + -0.7 230,234 + -0.2 230,235 - -0.5 230,235 - +0.1 230,235 - -2.6 230,235 - -0.4 230,345 + RR42_RS00975 0.13 -0.6 230,547 - RR42_RS00975 0.35 -0.7 230,547 - RR42_RS00975 0.35 -1.5 230,900 + RR42_RS00975 0.73 +0.6 230,900 + RR42_RS00975 0.73 -2.0 230,901 - RR42_RS00975 0.73 -0.3 231,141 - -0.3 231,141 - -0.3 231,392 + RR42_RS00980 0.28 -0.2 231,392 + RR42_RS00980 0.28 +0.2 231,392 + RR42_RS00980 0.28 -0.2 231,392 + RR42_RS00980 0.28 -0.2 231,393 - RR42_RS00980 0.28 +0.3 231,393 - RR42_RS00980 0.28 -1.5 231,393 - RR42_RS00980 0.28 -0.5 231,393 - RR42_RS00980 0.28 +0.2 231,470 + RR42_RS00980 0.41 -0.7 231,979 + RR42_RS00985 0.16 -0.1 232,011 + RR42_RS00985 0.21 -0.7 232,011 + RR42_RS00985 0.21 -0.5 232,011 + RR42_RS00985 0.21 -0.4 232,011 + RR42_RS00985 0.21 -1.0 232,013 + RR42_RS00985 0.21 +1.3 232,014 - RR42_RS00985 0.21 -0.7 232,014 - RR42_RS00985 0.21 +0.0 232,077 + RR42_RS00985 0.32 -1.5 232,078 - RR42_RS00985 0.32 -0.0 232,078 - RR42_RS00985 0.32 +0.4 232,091 - RR42_RS00985 0.34 -0.4 232,116 + RR42_RS00985 0.38 +2.4 232,116 + RR42_RS00985 0.38 -0.4 232,116 + RR42_RS00985 0.38 -0.6 232,116 + RR42_RS00985 0.38 -0.2 232,117 - RR42_RS00985 0.38 -0.3 232,152 + RR42_RS00985 0.44 +0.2 232,153 - RR42_RS00985 0.44 -0.0
Or see this region's nucleotide sequence