Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00835 and RR42_RS00840 are separated by 168 nucleotides RR42_RS00840 and RR42_RS00845 overlap by 4 nucleotides RR42_RS00845 and RR42_RS00850 overlap by 4 nucleotides RR42_RS00850 and RR42_RS00855 are separated by 532 nucleotides
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
RR42_RS00840: RR42_RS00840 - short-chain dehydrogenase, at 205,091 to 205,903
_RS00840
RR42_RS00845: RR42_RS00845 - metal ABC transporter substrate-binding protein, at 205,900 to 206,736
_RS00845
RR42_RS00850: RR42_RS00850 - hypothetical protein, at 206,733 to 206,993
_RS00850
RR42_RS00855: RR42_RS00855 - Zn-dependent hydrolase, at 207,526 to 208,599
_RS00855
Position (kb) 205
206
207 Strain fitness (log2 ratio) -2
-1
0
1 at 204.921 kb on - strand at 204.921 kb on - strand at 205.157 kb on + strand at 205.158 kb on - strand at 205.562 kb on + strand, within RR42_RS00840 at 205.563 kb on - strand, within RR42_RS00840 at 205.832 kb on + strand at 206.135 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.327 kb on + strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.328 kb on - strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.362 kb on + strand, within RR42_RS00845 at 206.363 kb on - strand, within RR42_RS00845 at 206.363 kb on - strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.407 kb on + strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.408 kb on - strand, within RR42_RS00845 at 206.409 kb on + strand, within RR42_RS00845 at 206.410 kb on - strand, within RR42_RS00845 at 206.506 kb on - strand, within RR42_RS00845 at 206.544 kb on + strand, within RR42_RS00845 at 206.544 kb on + strand, within RR42_RS00845 at 206.590 kb on + strand, within RR42_RS00845 at 206.707 kb on + strand at 206.707 kb on + strand at 206.707 kb on + strand at 206.708 kb on - strand at 206.708 kb on - strand at 206.708 kb on - strand at 206.734 kb on + strand at 207.534 kb on + strand at 207.541 kb on - strand at 207.643 kb on - strand, within RR42_RS00855 at 207.688 kb on - strand, within RR42_RS00855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 204,921 - +0.1 204,921 - +0.7 205,157 + +0.4 205,158 - +0.5 205,562 + RR42_RS00840 0.58 +0.2 205,563 - RR42_RS00840 0.58 -1.3 205,832 + +1.0 206,135 + RR42_RS00845 0.28 +1.4 206,327 + RR42_RS00845 0.51 +1.0 206,327 + RR42_RS00845 0.51 +0.2 206,327 + RR42_RS00845 0.51 -0.2 206,328 - RR42_RS00845 0.51 +0.8 206,328 - RR42_RS00845 0.51 +0.2 206,328 - RR42_RS00845 0.51 +0.7 206,328 - RR42_RS00845 0.51 +0.4 206,328 - RR42_RS00845 0.51 +0.7 206,328 - RR42_RS00845 0.51 +0.2 206,362 + RR42_RS00845 0.55 +0.5 206,362 + RR42_RS00845 0.55 +0.2 206,362 + RR42_RS00845 0.55 +0.9 206,362 + RR42_RS00845 0.55 -0.5 206,363 - RR42_RS00845 0.55 +1.4 206,363 - RR42_RS00845 0.55 +0.4 206,407 + RR42_RS00845 0.61 -0.3 206,407 + RR42_RS00845 0.61 -0.0 206,407 + RR42_RS00845 0.61 +0.6 206,407 + RR42_RS00845 0.61 +1.3 206,407 + RR42_RS00845 0.61 -0.6 206,407 + RR42_RS00845 0.61 -0.0 206,407 + RR42_RS00845 0.61 -0.4 206,407 + RR42_RS00845 0.61 -0.6 206,407 + RR42_RS00845 0.61 +0.8 206,408 - RR42_RS00845 0.61 +0.4 206,408 - RR42_RS00845 0.61 -1.1 206,408 - RR42_RS00845 0.61 +0.4 206,408 - RR42_RS00845 0.61 -0.2 206,408 - RR42_RS00845 0.61 -0.1 206,408 - RR42_RS00845 0.61 +0.8 206,408 - RR42_RS00845 0.61 -2.0 206,408 - RR42_RS00845 0.61 -0.7 206,409 + RR42_RS00845 0.61 +1.2 206,410 - RR42_RS00845 0.61 -0.3 206,506 - RR42_RS00845 0.72 -0.6 206,544 + RR42_RS00845 0.77 +0.3 206,544 + RR42_RS00845 0.77 -0.7 206,590 + RR42_RS00845 0.82 +1.2 206,707 + -1.0 206,707 + +0.7 206,707 + +0.3 206,708 - -0.4 206,708 - -0.3 206,708 - -0.9 206,734 + +0.5 207,534 + +0.2 207,541 - +0.6 207,643 - RR42_RS00855 0.11 +0.4 207,688 - RR42_RS00855 0.15 +1.1
Or see this region's nucleotide sequence