Experiment: R2A with Polymyxin B sulfate 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00660 and RR42_RS00665 are separated by 70 nucleotides RR42_RS00665 and RR42_RS00670 are separated by 116 nucleotides RR42_RS00670 and RR42_RS00675 are separated by 11 nucleotides RR42_RS00675 and RR42_RS00680 are separated by 57 nucleotides
RR42_RS00660: RR42_RS00660 - permease, at 169,954 to 170,904
_RS00660
RR42_RS00665: RR42_RS00665 - pyrrolidone-carboxylate peptidase, at 170,975 to 171,649
_RS00665
RR42_RS00670: RR42_RS00670 - teicoplanin resistance protein VanZ, at 171,766 to 172,950
_RS00670
RR42_RS00675: RR42_RS00675 - ABC transporter, at 172,962 to 173,654
_RS00675
RR42_RS00680: RR42_RS00680 - ABC transporter permease, at 173,712 to 174,653
_RS00680
Position (kb)
171
172
173 Strain fitness (log2 ratio)
-1
0
1
2 at 170.878 kb on - strand at 170.878 kb on - strand at 170.890 kb on + strand at 170.890 kb on + strand at 170.903 kb on - strand at 170.903 kb on - strand at 170.903 kb on - strand at 171.329 kb on + strand, within RR42_RS00665 at 171.329 kb on + strand, within RR42_RS00665 at 171.437 kb on + strand, within RR42_RS00665 at 171.438 kb on - strand, within RR42_RS00665 at 171.438 kb on - strand, within RR42_RS00665 at 171.502 kb on + strand, within RR42_RS00665 at 171.510 kb on - strand, within RR42_RS00665 at 171.822 kb on - strand at 171.822 kb on - strand at 171.822 kb on - strand at 171.822 kb on - strand at 171.923 kb on + strand, within RR42_RS00670 at 171.924 kb on - strand, within RR42_RS00670 at 171.924 kb on - strand, within RR42_RS00670 at 172.319 kb on - strand, within RR42_RS00670 at 172.319 kb on - strand, within RR42_RS00670 at 172.730 kb on + strand, within RR42_RS00670 at 172.731 kb on - strand, within RR42_RS00670 at 172.850 kb on + strand at 172.850 kb on + strand at 172.851 kb on - strand at 172.851 kb on - strand at 172.963 kb on + strand at 173.116 kb on - strand, within RR42_RS00675 at 173.311 kb on + strand, within RR42_RS00675 at 173.339 kb on - strand, within RR42_RS00675 at 173.353 kb on + strand, within RR42_RS00675 at 173.353 kb on + strand, within RR42_RS00675 at 173.378 kb on + strand, within RR42_RS00675 at 173.458 kb on + strand, within RR42_RS00675 at 173.458 kb on + strand, within RR42_RS00675 at 173.458 kb on + strand, within RR42_RS00675 at 173.459 kb on - strand, within RR42_RS00675 at 173.459 kb on - strand, within RR42_RS00675 at 173.680 kb on + strand at 173.680 kb on + strand at 173.680 kb on + strand at 173.680 kb on + strand at 173.680 kb on + strand at 173.681 kb on - strand at 173.681 kb on - strand at 173.681 kb on - strand at 173.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.005 mg/ml remove 170,878 - -0.4 170,878 - -0.5 170,890 + +1.0 170,890 + +0.6 170,903 - +0.1 170,903 - +0.7 170,903 - -0.5 171,329 + RR42_RS00665 0.52 -0.3 171,329 + RR42_RS00665 0.52 -0.0 171,437 + RR42_RS00665 0.68 -0.1 171,438 - RR42_RS00665 0.69 -0.8 171,438 - RR42_RS00665 0.69 -0.6 171,502 + RR42_RS00665 0.78 +0.0 171,510 - RR42_RS00665 0.79 +0.1 171,822 - +0.0 171,822 - -0.1 171,822 - +0.5 171,822 - +0.6 171,923 + RR42_RS00670 0.13 +0.8 171,924 - RR42_RS00670 0.13 +0.5 171,924 - RR42_RS00670 0.13 +0.4 172,319 - RR42_RS00670 0.47 +0.4 172,319 - RR42_RS00670 0.47 -0.6 172,730 + RR42_RS00670 0.81 +0.5 172,731 - RR42_RS00670 0.81 +0.3 172,850 + +0.5 172,850 + -0.2 172,851 - +0.5 172,851 - +0.9 172,963 + +0.0 173,116 - RR42_RS00675 0.22 -1.5 173,311 + RR42_RS00675 0.50 +0.0 173,339 - RR42_RS00675 0.54 +0.4 173,353 + RR42_RS00675 0.56 -0.3 173,353 + RR42_RS00675 0.56 -1.7 173,378 + RR42_RS00675 0.60 +1.7 173,458 + RR42_RS00675 0.72 +1.0 173,458 + RR42_RS00675 0.72 +0.3 173,458 + RR42_RS00675 0.72 -0.0 173,459 - RR42_RS00675 0.72 +2.0 173,459 - RR42_RS00675 0.72 -1.0 173,680 + +0.1 173,680 + -1.3 173,680 + +0.9 173,680 + -0.8 173,680 + +0.5 173,681 - -0.7 173,681 - -0.1 173,681 - +0.0 173,681 - -0.9
Or see this region's nucleotide sequence