Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5600 and Pf1N1B4_5601 are separated by 39 nucleotides Pf1N1B4_5601 and Pf1N1B4_5602 are separated by 117 nucleotides
Pf1N1B4_5600 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 22,486 to 23,589
_5600
Pf1N1B4_5601 - FIG00966546: hypothetical protein, at 23,629 to 24,966
_5601
Pf1N1B4_5602 - 6-aminohexanoate-dimer hydrolase (EC 3.5.1.46), at 25,084 to 26,346
_5602
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.707 kb on - strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.721 kb on + strand, within Pf1N1B4_5600 at 22.722 kb on - strand, within Pf1N1B4_5600 at 22.932 kb on - strand, within Pf1N1B4_5600 at 22.932 kb on - strand, within Pf1N1B4_5600 at 23.018 kb on + strand, within Pf1N1B4_5600 at 23.019 kb on - strand at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.019 kb on - strand, within Pf1N1B4_5600 at 23.199 kb on - strand at 23.210 kb on + strand, within Pf1N1B4_5600 at 23.424 kb on - strand, within Pf1N1B4_5600 at 23.424 kb on - strand, within Pf1N1B4_5600 at 23.551 kb on + strand at 23.552 kb on - strand at 23.571 kb on + strand at 23.630 kb on + strand at 23.634 kb on - strand at 23.648 kb on + strand at 23.648 kb on + strand at 23.649 kb on - strand at 23.649 kb on - strand at 23.724 kb on - strand at 23.736 kb on + strand at 23.739 kb on - strand at 23.739 kb on - strand at 23.819 kb on + strand, within Pf1N1B4_5601 at 23.846 kb on + strand, within Pf1N1B4_5601 at 23.933 kb on + strand, within Pf1N1B4_5601 at 23.933 kb on + strand at 24.128 kb on + strand, within Pf1N1B4_5601 at 24.227 kb on + strand, within Pf1N1B4_5601 at 24.227 kb on + strand, within Pf1N1B4_5601 at 24.228 kb on - strand, within Pf1N1B4_5601 at 24.333 kb on - strand, within Pf1N1B4_5601 at 24.533 kb on + strand, within Pf1N1B4_5601 at 24.533 kb on + strand, within Pf1N1B4_5601 at 24.534 kb on - strand, within Pf1N1B4_5601 at 24.584 kb on + strand, within Pf1N1B4_5601 at 24.585 kb on - strand, within Pf1N1B4_5601 at 24.737 kb on + strand, within Pf1N1B4_5601 at 24.737 kb on + strand at 24.738 kb on - strand, within Pf1N1B4_5601 at 24.738 kb on - strand, within Pf1N1B4_5601 at 24.738 kb on - strand, within Pf1N1B4_5601 at 24.966 kb on + strand at 25.073 kb on + strand at 25.093 kb on - strand at 25.265 kb on + strand, within Pf1N1B4_5602 at 25.266 kb on - strand, within Pf1N1B4_5602 at 25.326 kb on - strand, within Pf1N1B4_5602 at 25.613 kb on + strand, within Pf1N1B4_5602 at 25.614 kb on - strand, within Pf1N1B4_5602 at 25.661 kb on + strand, within Pf1N1B4_5602 at 25.667 kb on + strand, within Pf1N1B4_5602 at 25.688 kb on + strand, within Pf1N1B4_5602 at 25.689 kb on - strand, within Pf1N1B4_5602 at 25.758 kb on - strand, within Pf1N1B4_5602 at 25.770 kb on - strand, within Pf1N1B4_5602 at 25.848 kb on - strand, within Pf1N1B4_5602 at 25.848 kb on - strand, within Pf1N1B4_5602 at 25.941 kb on + strand, within Pf1N1B4_5602
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 22,707 - Pf1N1B4_5600 0.20 -0.7 22,707 - Pf1N1B4_5600 0.20 +2.9 22,707 - Pf1N1B4_5600 0.20 -0.9 22,721 + Pf1N1B4_5600 0.21 -1.0 22,721 + Pf1N1B4_5600 0.21 +0.7 22,721 + Pf1N1B4_5600 0.21 +1.0 22,722 - Pf1N1B4_5600 0.21 +0.7 22,932 - Pf1N1B4_5600 0.40 -0.3 22,932 - Pf1N1B4_5600 0.40 -0.3 23,018 + Pf1N1B4_5600 0.48 +1.9 23,019 - +0.7 23,019 - Pf1N1B4_5600 0.48 +1.1 23,019 - Pf1N1B4_5600 0.48 +0.4 23,019 - Pf1N1B4_5600 0.48 +0.7 23,199 - +1.7 23,210 + Pf1N1B4_5600 0.66 -0.3 23,424 - Pf1N1B4_5600 0.85 -0.3 23,424 - Pf1N1B4_5600 0.85 -1.6 23,551 + +1.3 23,552 - +0.8 23,571 + -1.9 23,630 + +1.3 23,634 - +0.1 23,648 + +1.6 23,648 + +0.4 23,649 - -0.7 23,649 - +0.7 23,724 - +1.2 23,736 + -1.3 23,739 - +2.0 23,739 - -0.3 23,819 + Pf1N1B4_5601 0.14 +1.3 23,846 + Pf1N1B4_5601 0.16 +1.3 23,933 + Pf1N1B4_5601 0.23 -0.3 23,933 + +1.3 24,128 + Pf1N1B4_5601 0.37 +1.0 24,227 + Pf1N1B4_5601 0.45 +1.6 24,227 + Pf1N1B4_5601 0.45 +0.1 24,228 - Pf1N1B4_5601 0.45 +0.7 24,333 - Pf1N1B4_5601 0.53 -1.3 24,533 + Pf1N1B4_5601 0.68 -0.8 24,533 + Pf1N1B4_5601 0.68 +0.3 24,534 - Pf1N1B4_5601 0.68 -0.3 24,584 + Pf1N1B4_5601 0.71 +2.5 24,585 - Pf1N1B4_5601 0.71 +0.3 24,737 + Pf1N1B4_5601 0.83 +0.3 24,737 + +0.7 24,738 - Pf1N1B4_5601 0.83 +2.0 24,738 - Pf1N1B4_5601 0.83 +1.5 24,738 - Pf1N1B4_5601 0.83 +0.9 24,966 + +0.9 25,073 + -1.6 25,093 - +1.4 25,265 + Pf1N1B4_5602 0.14 -0.5 25,266 - Pf1N1B4_5602 0.14 +1.0 25,326 - Pf1N1B4_5602 0.19 +2.2 25,613 + Pf1N1B4_5602 0.42 -0.3 25,614 - Pf1N1B4_5602 0.42 +0.1 25,661 + Pf1N1B4_5602 0.46 -1.0 25,667 + Pf1N1B4_5602 0.46 -1.0 25,688 + Pf1N1B4_5602 0.48 +1.3 25,689 - Pf1N1B4_5602 0.48 -0.3 25,758 - Pf1N1B4_5602 0.53 +2.0 25,770 - Pf1N1B4_5602 0.54 -2.6 25,848 - Pf1N1B4_5602 0.60 -0.7 25,848 - Pf1N1B4_5602 0.60 +1.0 25,941 + Pf1N1B4_5602 0.68 +0.4
Or see this region's nucleotide sequence