Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_514 and Pf1N1B4_515 are separated by 256 nucleotides Pf1N1B4_515 and Pf1N1B4_516 overlap by 1 nucleotides
Pf1N1B4_514 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 605,492 to 606,226
_514
Pf1N1B4_515 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 606,483 to 607,241
_515
Pf1N1B4_516 - Sensor histidine kinase, at 607,241 to 608,293
_516
Position (kb) 606
607
608 Strain fitness (log2 ratio) -2
-1
0
1
2 at 605.483 kb on + strand at 605.493 kb on + strand at 606.294 kb on + strand at 606.294 kb on + strand at 606.295 kb on - strand at 606.415 kb on + strand at 606.416 kb on - strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.442 kb on - strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.536 kb on + strand at 606.604 kb on - strand, within Pf1N1B4_515 at 606.621 kb on - strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.955 kb on + strand at 607.213 kb on - strand at 607.213 kb on - strand at 607.258 kb on + strand at 607.302 kb on + strand at 607.302 kb on + strand at 607.303 kb on - strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.833 kb on - strand at 607.833 kb on - strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 605,483 + -1.6 605,493 + +0.1 606,294 + +0.5 606,294 + -0.1 606,295 - +0.1 606,415 + -2.1 606,416 - +0.6 606,441 + -0.5 606,441 + +0.3 606,441 + +0.9 606,442 - -0.3 606,461 + -0.5 606,461 + -0.4 606,461 + +1.9 606,536 + +2.5 606,604 - Pf1N1B4_515 0.16 +1.9 606,621 - Pf1N1B4_515 0.18 +1.1 606,837 + Pf1N1B4_515 0.47 -0.1 606,837 + Pf1N1B4_515 0.47 +1.4 606,837 + Pf1N1B4_515 0.47 +0.7 606,837 + Pf1N1B4_515 0.47 -0.1 606,838 - Pf1N1B4_515 0.47 -2.4 606,838 - Pf1N1B4_515 0.47 +0.9 606,838 - +0.5 606,838 - Pf1N1B4_515 0.47 +0.6 606,838 - Pf1N1B4_515 0.47 +0.9 606,955 + +1.5 607,213 - +0.1 607,213 - -0.1 607,258 + -0.1 607,302 + +0.7 607,302 + +0.9 607,303 - +0.6 607,832 + Pf1N1B4_516 0.56 -1.4 607,832 + Pf1N1B4_516 0.56 -2.4 607,832 + +1.5 607,832 + Pf1N1B4_516 0.56 +0.1 607,833 - +1.3 607,833 - Pf1N1B4_516 0.56 +1.7 608,158 + Pf1N1B4_516 0.87 -0.5 608,158 + Pf1N1B4_516 0.87 +0.5
Or see this region's nucleotide sequence