Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4789

Experiment: L-Valine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4787 and Pf1N1B4_4788 are separated by 14 nucleotidesPf1N1B4_4788 and Pf1N1B4_4789 are separated by 136 nucleotidesPf1N1B4_4789 and Pf1N1B4_4790 are separated by 3 nucleotides Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 5,289,645 to 5,290,772 _4787 Pf1N1B4_4788 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,290,787 to 5,291,560 _4788 Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848 _4789 Pf1N1B4_4790 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data), at 5,292,852 to 5,293,922 _4790 Position (kb) 5291 5292 5293Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5290.722 kb on + strandat 5290.723 kb on - strandat 5290.723 kb on - strandat 5290.723 kb on - strandat 5290.786 kb on + strandat 5290.786 kb on + strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.787 kb on - strandat 5290.794 kb on - strandat 5291.021 kb on + strand, within Pf1N1B4_4788at 5291.022 kb on - strand, within Pf1N1B4_4788at 5291.525 kb on - strandat 5291.558 kb on + strandat 5291.558 kb on + strandat 5291.708 kb on - strandat 5291.708 kb on - strandat 5291.743 kb on - strandat 5293.182 kb on - strand, within Pf1N1B4_4790at 5293.366 kb on + strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.388 kb on - strand, within Pf1N1B4_4790at 5293.432 kb on + strand, within Pf1N1B4_4790at 5293.432 kb on + strand, within Pf1N1B4_4790at 5293.433 kb on - strand, within Pf1N1B4_4790at 5293.433 kb on - strand, within Pf1N1B4_4790at 5293.649 kb on - strand, within Pf1N1B4_4790

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (C)
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5,290,722 + +1.6
5,290,723 - -2.0
5,290,723 - -2.7
5,290,723 - -0.4
5,290,786 + -1.1
5,290,786 + -3.4
5,290,787 - -2.7
5,290,787 - -2.7
5,290,787 - -0.4
5,290,787 - -2.0
5,290,794 - -2.4
5,291,021 + Pf1N1B4_4788 0.30 -2.7
5,291,022 - Pf1N1B4_4788 0.30 -2.0
5,291,525 - -2.4
5,291,558 + -1.4
5,291,558 + -1.1
5,291,708 - -1.4
5,291,708 - +0.9
5,291,743 - -1.4
5,293,182 - Pf1N1B4_4790 0.31 -2.3
5,293,366 + Pf1N1B4_4790 0.48 -5.5
5,293,388 - Pf1N1B4_4790 0.50 -2.7
5,293,388 - Pf1N1B4_4790 0.50 -3.7
5,293,388 - Pf1N1B4_4790 0.50 -1.4
5,293,432 + Pf1N1B4_4790 0.54 -3.9
5,293,432 + Pf1N1B4_4790 0.54 -0.6
5,293,433 - Pf1N1B4_4790 0.54 -2.4
5,293,433 - Pf1N1B4_4790 0.54 -1.4
5,293,649 - Pf1N1B4_4790 0.74 -2.0

Or see this region's nucleotide sequence