Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4787 and Pf1N1B4_4788 are separated by 14 nucleotides Pf1N1B4_4788 and Pf1N1B4_4789 are separated by 136 nucleotides Pf1N1B4_4789 and Pf1N1B4_4790 are separated by 3 nucleotides
Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,289,645 to 5,290,772
_4787
Pf1N1B4_4788 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,290,787 to 5,291,560
_4788
Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848
_4789
Pf1N1B4_4790 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 5,292,852 to 5,293,922
_4790
Position (kb)
5291
5292
5293 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5290.722 kb on + strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.786 kb on + strand at 5290.786 kb on + strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.794 kb on - strand at 5291.021 kb on + strand, within Pf1N1B4_4788 at 5291.022 kb on - strand, within Pf1N1B4_4788 at 5291.525 kb on - strand at 5291.558 kb on + strand at 5291.558 kb on + strand at 5291.708 kb on - strand at 5291.708 kb on - strand at 5291.743 kb on - strand at 5293.182 kb on - strand, within Pf1N1B4_4790 at 5293.366 kb on + strand, within Pf1N1B4_4790 at 5293.388 kb on - strand, within Pf1N1B4_4790 at 5293.388 kb on - strand, within Pf1N1B4_4790 at 5293.388 kb on - strand, within Pf1N1B4_4790 at 5293.432 kb on + strand, within Pf1N1B4_4790 at 5293.432 kb on + strand, within Pf1N1B4_4790 at 5293.433 kb on - strand, within Pf1N1B4_4790 at 5293.433 kb on - strand, within Pf1N1B4_4790 at 5293.649 kb on - strand, within Pf1N1B4_4790
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 5,290,722 + +1.6 5,290,723 - -2.0 5,290,723 - -2.7 5,290,723 - -0.4 5,290,786 + -1.1 5,290,786 + -3.4 5,290,787 - -2.7 5,290,787 - -2.7 5,290,787 - -0.4 5,290,787 - -2.0 5,290,794 - -2.4 5,291,021 + Pf1N1B4_4788 0.30 -2.7 5,291,022 - Pf1N1B4_4788 0.30 -2.0 5,291,525 - -2.4 5,291,558 + -1.4 5,291,558 + -1.1 5,291,708 - -1.4 5,291,708 - +0.9 5,291,743 - -1.4 5,293,182 - Pf1N1B4_4790 0.31 -2.3 5,293,366 + Pf1N1B4_4790 0.48 -5.5 5,293,388 - Pf1N1B4_4790 0.50 -2.7 5,293,388 - Pf1N1B4_4790 0.50 -3.7 5,293,388 - Pf1N1B4_4790 0.50 -1.4 5,293,432 + Pf1N1B4_4790 0.54 -3.9 5,293,432 + Pf1N1B4_4790 0.54 -0.6 5,293,433 - Pf1N1B4_4790 0.54 -2.4 5,293,433 - Pf1N1B4_4790 0.54 -1.4 5,293,649 - Pf1N1B4_4790 0.74 -2.0
Or see this region's nucleotide sequence