Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4641 and Pf1N1B4_4642 are separated by 186 nucleotides Pf1N1B4_4642 and Pf1N1B4_4643 are separated by 203 nucleotides Pf1N1B4_4643 and Pf1N1B4_4644 are separated by 370 nucleotides Pf1N1B4_4644 and Pf1N1B4_4645 are separated by 245 nucleotides
Pf1N1B4_4641 - FIG00954580: hypothetical protein, at 5,118,110 to 5,118,463
_4641
Pf1N1B4_4642 - hypothetical protein, at 5,118,650 to 5,118,763
_4642
Pf1N1B4_4643 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form, at 5,118,967 to 5,123,874
_4643
Pf1N1B4_4644 - hypothetical protein, at 5,124,245 to 5,124,505
_4644
Pf1N1B4_4645 - FIG00954888: hypothetical protein, at 5,124,751 to 5,125,044
_4645
Position (kb)
5118
5119
5120
5121
5122
5123
5124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.126 kb on + strand at 5118.127 kb on - strand at 5118.127 kb on - strand at 5118.130 kb on + strand at 5118.620 kb on + strand at 5119.093 kb on - strand at 5119.093 kb on - strand at 5119.216 kb on + strand at 5119.332 kb on + strand at 5119.726 kb on + strand, within Pf1N1B4_4643 at 5119.726 kb on + strand, within Pf1N1B4_4643 at 5119.816 kb on + strand, within Pf1N1B4_4643 at 5119.816 kb on + strand, within Pf1N1B4_4643 at 5119.817 kb on - strand, within Pf1N1B4_4643 at 5119.895 kb on - strand, within Pf1N1B4_4643 at 5119.899 kb on - strand, within Pf1N1B4_4643 at 5119.960 kb on + strand, within Pf1N1B4_4643 at 5119.961 kb on - strand at 5120.002 kb on + strand, within Pf1N1B4_4643 at 5120.003 kb on - strand, within Pf1N1B4_4643 at 5120.225 kb on - strand, within Pf1N1B4_4643 at 5120.254 kb on + strand at 5120.254 kb on + strand, within Pf1N1B4_4643 at 5120.255 kb on - strand at 5120.635 kb on + strand, within Pf1N1B4_4643 at 5120.687 kb on - strand, within Pf1N1B4_4643 at 5121.028 kb on + strand, within Pf1N1B4_4643 at 5121.367 kb on + strand, within Pf1N1B4_4643 at 5121.477 kb on - strand, within Pf1N1B4_4643 at 5121.826 kb on - strand, within Pf1N1B4_4643 at 5121.866 kb on + strand, within Pf1N1B4_4643 at 5121.866 kb on + strand, within Pf1N1B4_4643 at 5121.867 kb on - strand, within Pf1N1B4_4643 at 5121.867 kb on - strand, within Pf1N1B4_4643 at 5122.102 kb on + strand at 5122.285 kb on + strand, within Pf1N1B4_4643 at 5122.285 kb on + strand, within Pf1N1B4_4643 at 5122.285 kb on + strand, within Pf1N1B4_4643 at 5122.286 kb on - strand, within Pf1N1B4_4643 at 5122.286 kb on - strand, within Pf1N1B4_4643 at 5122.286 kb on - strand, within Pf1N1B4_4643 at 5122.286 kb on - strand at 5122.563 kb on + strand, within Pf1N1B4_4643 at 5122.621 kb on + strand, within Pf1N1B4_4643 at 5122.621 kb on + strand, within Pf1N1B4_4643 at 5122.622 kb on - strand, within Pf1N1B4_4643 at 5122.622 kb on - strand, within Pf1N1B4_4643 at 5122.622 kb on - strand, within Pf1N1B4_4643 at 5122.822 kb on + strand, within Pf1N1B4_4643 at 5123.077 kb on + strand, within Pf1N1B4_4643 at 5123.078 kb on - strand, within Pf1N1B4_4643 at 5123.078 kb on - strand, within Pf1N1B4_4643 at 5123.078 kb on - strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.416 kb on + strand at 5123.416 kb on + strand at 5123.416 kb on + strand at 5123.417 kb on - strand at 5123.485 kb on + strand at 5123.485 kb on + strand at 5123.767 kb on + strand at 5123.767 kb on + strand at 5123.767 kb on + strand at 5124.077 kb on + strand at 5124.078 kb on - strand at 5124.210 kb on + strand at 5124.211 kb on - strand at 5124.217 kb on - strand at 5124.232 kb on - strand at 5124.247 kb on - strand at 5124.285 kb on + strand at 5124.285 kb on + strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.545 kb on + strand at 5124.545 kb on + strand at 5124.546 kb on - strand at 5124.611 kb on + strand at 5124.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 5,118,126 + +2.2 5,118,126 + -1.0 5,118,126 + -0.0 5,118,126 + +1.0 5,118,126 + -0.4 5,118,126 + +0.6 5,118,126 + +1.2 5,118,126 + -0.4 5,118,126 + -0.5 5,118,126 + -1.0 5,118,127 - +0.3 5,118,127 - -1.0 5,118,130 + -0.6 5,118,620 + +1.9 5,119,093 - -2.0 5,119,093 - -0.8 5,119,216 + -0.4 5,119,332 + -2.4 5,119,726 + Pf1N1B4_4643 0.15 -0.4 5,119,726 + Pf1N1B4_4643 0.15 -2.7 5,119,816 + Pf1N1B4_4643 0.17 -1.0 5,119,816 + Pf1N1B4_4643 0.17 -3.0 5,119,817 - Pf1N1B4_4643 0.17 +1.9 5,119,895 - Pf1N1B4_4643 0.19 +0.6 5,119,899 - Pf1N1B4_4643 0.19 -1.1 5,119,960 + Pf1N1B4_4643 0.20 -2.0 5,119,961 - -1.4 5,120,002 + Pf1N1B4_4643 0.21 -2.7 5,120,003 - Pf1N1B4_4643 0.21 -2.7 5,120,225 - Pf1N1B4_4643 0.26 -0.8 5,120,254 + -1.4 5,120,254 + Pf1N1B4_4643 0.26 -2.2 5,120,255 - -2.0 5,120,635 + Pf1N1B4_4643 0.34 -1.3 5,120,687 - Pf1N1B4_4643 0.35 -1.6 5,121,028 + Pf1N1B4_4643 0.42 -2.0 5,121,367 + Pf1N1B4_4643 0.49 -1.5 5,121,477 - Pf1N1B4_4643 0.51 -0.4 5,121,826 - Pf1N1B4_4643 0.58 -0.4 5,121,866 + Pf1N1B4_4643 0.59 -1.0 5,121,866 + Pf1N1B4_4643 0.59 -2.0 5,121,867 - Pf1N1B4_4643 0.59 +0.6 5,121,867 - Pf1N1B4_4643 0.59 -2.2 5,122,102 + -1.4 5,122,285 + Pf1N1B4_4643 0.68 -1.4 5,122,285 + Pf1N1B4_4643 0.68 +0.6 5,122,285 + Pf1N1B4_4643 0.68 +0.9 5,122,286 - Pf1N1B4_4643 0.68 -0.4 5,122,286 - Pf1N1B4_4643 0.68 -2.0 5,122,286 - Pf1N1B4_4643 0.68 -3.0 5,122,286 - -1.4 5,122,563 + Pf1N1B4_4643 0.73 +0.4 5,122,621 + Pf1N1B4_4643 0.74 -0.4 5,122,621 + Pf1N1B4_4643 0.74 -2.0 5,122,622 - Pf1N1B4_4643 0.74 +1.6 5,122,622 - Pf1N1B4_4643 0.74 -1.0 5,122,622 - Pf1N1B4_4643 0.74 +0.2 5,122,822 + Pf1N1B4_4643 0.79 -2.2 5,123,077 + Pf1N1B4_4643 0.84 -0.8 5,123,078 - Pf1N1B4_4643 0.84 -0.4 5,123,078 - Pf1N1B4_4643 0.84 +1.2 5,123,078 - Pf1N1B4_4643 0.84 -0.4 5,123,356 + Pf1N1B4_4643 0.89 -0.4 5,123,356 + Pf1N1B4_4643 0.89 -1.0 5,123,356 + Pf1N1B4_4643 0.89 +0.6 5,123,356 + Pf1N1B4_4643 0.89 -2.0 5,123,356 + Pf1N1B4_4643 0.89 -1.4 5,123,356 + Pf1N1B4_4643 0.89 +0.1 5,123,416 + +0.2 5,123,416 + +0.9 5,123,416 + -0.4 5,123,417 - +0.6 5,123,485 + -1.1 5,123,485 + +0.3 5,123,767 + -2.4 5,123,767 + +0.6 5,123,767 + +0.0 5,124,077 + -0.7 5,124,078 - +0.6 5,124,210 + -0.7 5,124,211 - -1.4 5,124,217 - -0.0 5,124,232 - -1.1 5,124,247 - +0.0 5,124,285 + -0.4 5,124,285 + Pf1N1B4_4644 0.15 +0.1 5,124,286 - Pf1N1B4_4644 0.16 +0.3 5,124,286 - +1.8 5,124,286 - Pf1N1B4_4644 0.16 -1.4 5,124,286 - +0.3 5,124,286 - Pf1N1B4_4644 0.16 -0.4 5,124,286 - Pf1N1B4_4644 0.16 +0.6 5,124,286 - +0.0 5,124,286 - Pf1N1B4_4644 0.16 -0.4 5,124,286 - Pf1N1B4_4644 0.16 -0.7 5,124,286 - +1.6 5,124,545 + +0.6 5,124,545 + +2.2 5,124,546 - +0.6 5,124,611 + +0.4 5,124,611 + +0.0
Or see this region's nucleotide sequence