Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4051 and Pf1N1B4_4052 are separated by 493 nucleotides Pf1N1B4_4052 and Pf1N1B4_4053 are separated by 61 nucleotides Pf1N1B4_4053 and Pf1N1B4_4054 are separated by 341 nucleotides Pf1N1B4_4054 and Pf1N1B4_4055 are separated by 30 nucleotides
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283
_4052
Pf1N1B4_4053 - Cold shock protein CspD, at 4,506,345 to 4,506,626
_4053
Pf1N1B4_4054 - ATP-dependent Clp protease adaptor protein ClpS, at 4,506,968 to 4,507,213
_4054
Pf1N1B4_4055 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 4,507,244 to 4,509,514
_4055
Position (kb)
4505
4506
4507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.506 kb on + strand at 4504.506 kb on + strand at 4504.508 kb on + strand at 4504.508 kb on + strand at 4504.560 kb on + strand at 4504.692 kb on - strand at 4504.727 kb on + strand at 4504.769 kb on + strand at 4504.770 kb on - strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.780 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.856 kb on + strand at 4504.856 kb on + strand at 4504.910 kb on + strand at 4504.910 kb on + strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.953 kb on - strand at 4504.971 kb on + strand at 4504.972 kb on - strand at 4504.972 kb on - strand at 4504.981 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.140 kb on + strand at 4505.141 kb on - strand at 4505.153 kb on - strand, within Pf1N1B4_4052 at 4505.237 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand at 4505.320 kb on - strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.511 kb on - strand at 4505.677 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.678 kb on + strand, within Pf1N1B4_4052 at 4505.679 kb on - strand, within Pf1N1B4_4052 at 4505.679 kb on - strand, within Pf1N1B4_4052 at 4505.918 kb on + strand, within Pf1N1B4_4052 at 4505.954 kb on + strand, within Pf1N1B4_4052 at 4505.955 kb on - strand, within Pf1N1B4_4052 at 4505.955 kb on - strand, within Pf1N1B4_4052 at 4506.065 kb on + strand, within Pf1N1B4_4052 at 4506.203 kb on + strand at 4506.203 kb on + strand at 4506.203 kb on + strand at 4506.204 kb on - strand at 4506.204 kb on - strand at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.281 kb on + strand at 4506.282 kb on - strand at 4506.487 kb on + strand, within Pf1N1B4_4053 at 4506.488 kb on - strand, within Pf1N1B4_4053 at 4506.512 kb on - strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.555 kb on + strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.556 kb on - strand, within Pf1N1B4_4053 at 4506.663 kb on + strand at 4506.664 kb on - strand at 4506.686 kb on + strand at 4506.687 kb on - strand at 4506.687 kb on - strand at 4506.698 kb on + strand at 4506.739 kb on - strand at 4506.821 kb on + strand at 4506.821 kb on + strand at 4506.822 kb on - strand at 4506.822 kb on - strand at 4506.876 kb on + strand at 4506.903 kb on + strand at 4506.903 kb on + strand at 4506.943 kb on + strand at 4506.954 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.971 kb on + strand at 4506.972 kb on - strand at 4506.972 kb on - strand at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.998 kb on + strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4506.999 kb on - strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.104 kb on + strand, within Pf1N1B4_4054 at 4507.105 kb on - strand, within Pf1N1B4_4054 at 4507.160 kb on + strand, within Pf1N1B4_4054 at 4507.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 4,504,073 + Pf1N1B4_4051 0.78 +0.2 4,504,073 + Pf1N1B4_4051 0.78 +1.5 4,504,472 + -1.8 4,504,472 + +0.7 4,504,472 + +0.7 4,504,506 + +1.5 4,504,506 + +1.3 4,504,508 + +0.1 4,504,508 + -0.4 4,504,560 + +1.9 4,504,692 - -1.3 4,504,727 + +0.7 4,504,769 + -1.3 4,504,770 - +0.7 4,504,771 + -0.7 4,504,771 + -1.3 4,504,771 + -0.9 4,504,771 + -0.3 4,504,771 + +0.3 4,504,771 + +0.1 4,504,771 + +0.9 4,504,771 + -0.8 4,504,771 + -1.1 4,504,771 + +3.1 4,504,771 + +0.3 4,504,771 + +1.5 4,504,771 + +0.7 4,504,771 + +1.0 4,504,771 + -0.1 4,504,771 + -0.8 4,504,771 + +0.6 4,504,772 - +0.6 4,504,772 - +0.6 4,504,772 - -0.0 4,504,772 - +0.4 4,504,772 - +1.3 4,504,772 - +1.1 4,504,775 + -0.3 4,504,775 + -0.7 4,504,775 + +2.1 4,504,775 + +0.8 4,504,775 + +3.2 4,504,775 + -1.3 4,504,775 + +1.3 4,504,775 + +0.2 4,504,776 - +0.3 4,504,776 - -2.3 4,504,776 - -0.3 4,504,776 - +0.7 4,504,776 - +0.6 4,504,776 - -0.6 4,504,776 - +1.4 4,504,780 - -0.1 4,504,805 - +1.5 4,504,805 - +1.7 4,504,805 - +0.2 4,504,830 + +0.6 4,504,830 + +1.3 4,504,830 + -0.7 4,504,856 + +1.2 4,504,856 + +1.3 4,504,910 + +1.5 4,504,910 + +1.1 4,504,911 - +0.7 4,504,911 - +0.5 4,504,911 - +0.3 4,504,911 - +0.5 4,504,911 - -0.6 4,504,911 - -0.3 4,504,952 + -0.3 4,504,952 + -0.6 4,504,952 + -0.3 4,504,952 + -0.3 4,504,952 + -0.4 4,504,953 - +0.3 4,504,971 + +0.5 4,504,972 - -0.3 4,504,972 - +1.2 4,504,981 - +1.3 4,505,022 - +0.7 4,505,022 - +1.7 4,505,022 - -1.7 4,505,140 + -0.3 4,505,141 - +0.0 4,505,153 - Pf1N1B4_4052 0.10 +1.7 4,505,237 + Pf1N1B4_4052 0.17 +1.7 4,505,315 + Pf1N1B4_4052 0.23 +2.6 4,505,315 + Pf1N1B4_4052 0.23 -0.0 4,505,315 + Pf1N1B4_4052 0.23 +0.3 4,505,315 + Pf1N1B4_4052 0.23 +0.5 4,505,316 - Pf1N1B4_4052 0.23 +0.4 4,505,316 - Pf1N1B4_4052 0.23 +0.7 4,505,319 + Pf1N1B4_4052 0.23 +0.2 4,505,319 + Pf1N1B4_4052 0.23 -0.3 4,505,319 + +1.3 4,505,320 - Pf1N1B4_4052 0.23 +1.3 4,505,405 + Pf1N1B4_4052 0.30 +0.7 4,505,405 + Pf1N1B4_4052 0.30 +0.7 4,505,405 + Pf1N1B4_4052 0.30 +2.9 4,505,405 + Pf1N1B4_4052 0.30 -0.3 4,505,405 + Pf1N1B4_4052 0.30 +0.3 4,505,405 + +1.5 4,505,406 - Pf1N1B4_4052 0.30 +0.7 4,505,406 - Pf1N1B4_4052 0.30 -0.3 4,505,510 + Pf1N1B4_4052 0.38 -2.1 4,505,510 + Pf1N1B4_4052 0.38 +0.9 4,505,510 + Pf1N1B4_4052 0.38 +0.3 4,505,511 - +1.3 4,505,677 + Pf1N1B4_4052 0.52 -0.8 4,505,678 + Pf1N1B4_4052 0.52 +0.7 4,505,678 + Pf1N1B4_4052 0.52 -0.3 4,505,678 + Pf1N1B4_4052 0.52 -1.3 4,505,679 - Pf1N1B4_4052 0.52 +1.3 4,505,679 - Pf1N1B4_4052 0.52 +0.7 4,505,918 + Pf1N1B4_4052 0.71 +0.2 4,505,954 + Pf1N1B4_4052 0.74 +0.6 4,505,955 - Pf1N1B4_4052 0.74 -1.3 4,505,955 - Pf1N1B4_4052 0.74 -1.3 4,506,065 + Pf1N1B4_4052 0.83 -1.3 4,506,203 + -1.0 4,506,203 + -0.3 4,506,203 + +1.7 4,506,204 - -0.3 4,506,204 - -1.0 4,506,281 + +2.1 4,506,281 + +0.1 4,506,281 + -1.3 4,506,282 - +1.3 4,506,487 + Pf1N1B4_4053 0.50 -0.3 4,506,488 - Pf1N1B4_4053 0.51 -1.9 4,506,512 - Pf1N1B4_4053 0.59 -0.3 4,506,555 + Pf1N1B4_4053 0.74 -0.0 4,506,555 + Pf1N1B4_4053 0.74 +1.7 4,506,556 - Pf1N1B4_4053 0.75 -0.3 4,506,556 - Pf1N1B4_4053 0.75 -0.9 4,506,556 - Pf1N1B4_4053 0.75 -0.9 4,506,663 + +0.4 4,506,664 - -0.9 4,506,686 + +0.7 4,506,687 - -1.9 4,506,687 - -0.3 4,506,698 + +1.8 4,506,739 - -0.7 4,506,821 + +0.7 4,506,821 + +0.7 4,506,822 - +0.7 4,506,822 - -1.1 4,506,876 + -2.9 4,506,903 + +0.1 4,506,903 + -0.9 4,506,943 + +0.4 4,506,954 + +0.6 4,506,971 + -0.6 4,506,971 + -0.6 4,506,971 + +0.2 4,506,971 + -0.9 4,506,971 + -0.7 4,506,971 + +0.7 4,506,971 + -0.1 4,506,971 + +0.1 4,506,971 + -1.1 4,506,971 + -0.3 4,506,971 + -1.3 4,506,971 + -0.5 4,506,972 - -1.3 4,506,972 - -0.3 4,506,998 + Pf1N1B4_4054 0.12 -0.6 4,506,998 + Pf1N1B4_4054 0.12 -0.3 4,506,998 + Pf1N1B4_4054 0.12 -1.4 4,506,998 + Pf1N1B4_4054 0.12 -0.5 4,506,998 + Pf1N1B4_4054 0.12 -0.9 4,506,999 - Pf1N1B4_4054 0.13 -1.9 4,506,999 - Pf1N1B4_4054 0.13 -1.3 4,507,104 + Pf1N1B4_4054 0.55 +0.1 4,507,104 + Pf1N1B4_4054 0.55 -0.1 4,507,104 + Pf1N1B4_4054 0.55 -0.9 4,507,104 + Pf1N1B4_4054 0.55 -1.6 4,507,104 + Pf1N1B4_4054 0.55 +0.6 4,507,104 + Pf1N1B4_4054 0.55 -0.9 4,507,104 + Pf1N1B4_4054 0.55 -0.3 4,507,104 + Pf1N1B4_4054 0.55 -0.6 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,104 + Pf1N1B4_4054 0.55 -1.0 4,507,105 - Pf1N1B4_4054 0.56 +0.7 4,507,160 + Pf1N1B4_4054 0.78 -0.9 4,507,244 - -2.3
Or see this region's nucleotide sequence