Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4018 and Pf1N1B4_4019 are separated by 49 nucleotides Pf1N1B4_4019 and Pf1N1B4_4020 are separated by 51 nucleotides
Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287
_4018
Pf1N1B4_4019 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family, at 4,470,337 to 4,471,530
_4019
Pf1N1B4_4020 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 4,471,582 to 4,474,083
_4020
Position (kb)
4470
4471
4472 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4469.871 kb on + strand, within Pf1N1B4_4018 at 4469.872 kb on - strand, within Pf1N1B4_4018 at 4470.208 kb on - strand at 4470.799 kb on + strand, within Pf1N1B4_4019 at 4470.799 kb on + strand, within Pf1N1B4_4019 at 4470.800 kb on - strand, within Pf1N1B4_4019 at 4470.800 kb on - strand at 4470.920 kb on + strand, within Pf1N1B4_4019 at 4470.921 kb on - strand, within Pf1N1B4_4019 at 4470.921 kb on - strand, within Pf1N1B4_4019 at 4471.581 kb on + strand at 4471.581 kb on + strand at 4471.581 kb on + strand at 4471.582 kb on - strand at 4471.582 kb on - strand at 4471.673 kb on - strand at 4472.054 kb on - strand at 4472.054 kb on - strand, within Pf1N1B4_4020 at 4472.197 kb on + strand, within Pf1N1B4_4020
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 4,469,871 + Pf1N1B4_4018 0.67 -1.8 4,469,872 - Pf1N1B4_4018 0.67 +0.0 4,470,208 - -0.8 4,470,799 + Pf1N1B4_4019 0.39 +1.3 4,470,799 + Pf1N1B4_4019 0.39 +0.4 4,470,800 - Pf1N1B4_4019 0.39 +2.1 4,470,800 - -0.3 4,470,920 + Pf1N1B4_4019 0.49 +1.6 4,470,921 - Pf1N1B4_4019 0.49 +1.0 4,470,921 - Pf1N1B4_4019 0.49 -2.3 4,471,581 + +1.7 4,471,581 + -1.3 4,471,581 + +1.2 4,471,582 - +2.1 4,471,582 - +0.7 4,471,673 - +0.8 4,472,054 - +2.1 4,472,054 - Pf1N1B4_4020 0.19 +1.9 4,472,197 + Pf1N1B4_4020 0.25 +0.5
Or see this region's nucleotide sequence