Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3986 and Pf1N1B4_3987 are separated by 12 nucleotides Pf1N1B4_3987 and Pf1N1B4_3988 are separated by 328 nucleotides
Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367
_3986
Pf1N1B4_3987 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,429,380 to 4,430,543
_3987
Pf1N1B4_3988 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,430,872 to 4,432,578
_3988
Position (kb)
4429
4430
4431 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4428.392 kb on + strand, within Pf1N1B4_3986 at 4428.783 kb on + strand, within Pf1N1B4_3986 at 4428.787 kb on + strand, within Pf1N1B4_3986 at 4428.788 kb on - strand, within Pf1N1B4_3986 at 4428.788 kb on - strand, within Pf1N1B4_3986 at 4428.946 kb on + strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.019 kb on - strand, within Pf1N1B4_3986 at 4429.223 kb on + strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.291 kb on - strand at 4429.454 kb on - strand at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.564 kb on + strand, within Pf1N1B4_3987 at 4429.630 kb on + strand, within Pf1N1B4_3987 at 4429.630 kb on + strand, within Pf1N1B4_3987 at 4429.637 kb on - strand, within Pf1N1B4_3987 at 4429.663 kb on + strand, within Pf1N1B4_3987 at 4429.663 kb on + strand, within Pf1N1B4_3987 at 4429.664 kb on - strand, within Pf1N1B4_3987 at 4429.664 kb on - strand, within Pf1N1B4_3987 at 4429.664 kb on - strand at 4429.738 kb on + strand, within Pf1N1B4_3987 at 4429.827 kb on + strand, within Pf1N1B4_3987 at 4429.828 kb on - strand, within Pf1N1B4_3987 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.024 kb on - strand at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.033 kb on - strand, within Pf1N1B4_3987 at 4430.082 kb on + strand, within Pf1N1B4_3987 at 4430.083 kb on - strand, within Pf1N1B4_3987 at 4430.086 kb on + strand, within Pf1N1B4_3987 at 4430.102 kb on + strand, within Pf1N1B4_3987 at 4430.200 kb on + strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.353 kb on + strand, within Pf1N1B4_3987 at 4430.537 kb on - strand at 4430.611 kb on + strand at 4430.611 kb on + strand at 4430.671 kb on - strand at 4430.671 kb on - strand at 4430.671 kb on - strand at 4430.753 kb on + strand at 4430.753 kb on + strand at 4430.753 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.758 kb on - strand at 4430.758 kb on - strand at 4430.758 kb on - strand at 4430.768 kb on - strand at 4430.847 kb on + strand at 4430.847 kb on + strand at 4430.848 kb on - strand at 4430.852 kb on + strand at 4430.905 kb on + strand at 4430.906 kb on - strand at 4431.220 kb on + strand, within Pf1N1B4_3988 at 4431.220 kb on + strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.511 kb on + strand, within Pf1N1B4_3988 at 4431.512 kb on - strand, within Pf1N1B4_3988
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 4,428,392 + Pf1N1B4_3986 0.39 +1.1 4,428,783 + Pf1N1B4_3986 0.64 -1.3 4,428,787 + Pf1N1B4_3986 0.64 -1.3 4,428,788 - Pf1N1B4_3986 0.64 +0.3 4,428,788 - Pf1N1B4_3986 0.64 -0.3 4,428,946 + Pf1N1B4_3986 0.74 -1.8 4,428,947 - Pf1N1B4_3986 0.74 -1.8 4,428,947 - Pf1N1B4_3986 0.74 -2.8 4,428,947 - Pf1N1B4_3986 0.74 -1.8 4,429,018 + Pf1N1B4_3986 0.78 -1.6 4,429,018 + Pf1N1B4_3986 0.78 -1.3 4,429,018 + Pf1N1B4_3986 0.78 +0.7 4,429,018 + Pf1N1B4_3986 0.78 +0.5 4,429,019 - Pf1N1B4_3986 0.78 -1.3 4,429,223 + -0.3 4,429,224 - -1.8 4,429,224 - -1.8 4,429,224 - -2.3 4,429,224 - +0.7 4,429,224 - -2.3 4,429,224 - -1.0 4,429,291 - -1.0 4,429,454 - -0.3 4,429,502 - Pf1N1B4_3987 0.10 -0.3 4,429,502 - Pf1N1B4_3987 0.10 -1.5 4,429,502 - Pf1N1B4_3987 0.10 -1.8 4,429,564 + Pf1N1B4_3987 0.16 -1.3 4,429,630 + Pf1N1B4_3987 0.21 -0.7 4,429,630 + Pf1N1B4_3987 0.21 +0.7 4,429,637 - Pf1N1B4_3987 0.22 +0.4 4,429,663 + Pf1N1B4_3987 0.24 +0.7 4,429,663 + Pf1N1B4_3987 0.24 -1.3 4,429,664 - Pf1N1B4_3987 0.24 +0.6 4,429,664 - Pf1N1B4_3987 0.24 -0.3 4,429,664 - -0.3 4,429,738 + Pf1N1B4_3987 0.31 -2.1 4,429,827 + Pf1N1B4_3987 0.38 +0.7 4,429,828 - Pf1N1B4_3987 0.38 -0.7 4,430,023 + Pf1N1B4_3987 0.55 -1.6 4,430,023 + Pf1N1B4_3987 0.55 +0.7 4,430,024 - -0.3 4,430,032 + Pf1N1B4_3987 0.56 +0.7 4,430,032 + Pf1N1B4_3987 0.56 -2.6 4,430,033 - Pf1N1B4_3987 0.56 -0.8 4,430,082 + Pf1N1B4_3987 0.60 +0.7 4,430,083 - Pf1N1B4_3987 0.60 +0.7 4,430,086 + Pf1N1B4_3987 0.61 -1.3 4,430,102 + Pf1N1B4_3987 0.62 -0.8 4,430,200 + Pf1N1B4_3987 0.70 -0.3 4,430,201 - Pf1N1B4_3987 0.71 -1.3 4,430,201 - Pf1N1B4_3987 0.71 -1.3 4,430,353 + Pf1N1B4_3987 0.84 -1.3 4,430,537 - +0.6 4,430,611 + -0.8 4,430,611 + -0.8 4,430,671 - -0.7 4,430,671 - -0.8 4,430,671 - +1.7 4,430,753 + -0.5 4,430,753 + +0.0 4,430,753 + -0.3 4,430,757 + +0.7 4,430,757 + -0.8 4,430,757 + -0.4 4,430,757 + -0.3 4,430,757 + +1.3 4,430,758 - -1.5 4,430,758 - +0.0 4,430,758 - -1.6 4,430,768 - +0.3 4,430,847 + +0.7 4,430,847 + -0.5 4,430,848 - +1.1 4,430,852 + -0.5 4,430,905 + +1.7 4,430,906 - -0.4 4,431,220 + Pf1N1B4_3988 0.20 +0.5 4,431,220 + Pf1N1B4_3988 0.20 -1.8 4,431,221 - Pf1N1B4_3988 0.20 +0.7 4,431,221 - Pf1N1B4_3988 0.20 +2.4 4,431,221 - Pf1N1B4_3988 0.20 +0.9 4,431,511 + Pf1N1B4_3988 0.37 -1.3 4,431,512 - Pf1N1B4_3988 0.37 +0.5
Or see this region's nucleotide sequence