Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3987

Experiment: L-Valine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3986 and Pf1N1B4_3987 are separated by 12 nucleotidesPf1N1B4_3987 and Pf1N1B4_3988 are separated by 328 nucleotides Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367 _3986 Pf1N1B4_3987 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,429,380 to 4,430,543 _3987 Pf1N1B4_3988 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,430,872 to 4,432,578 _3988 Position (kb) 4429 4430 4431Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4428.392 kb on + strand, within Pf1N1B4_3986at 4428.783 kb on + strand, within Pf1N1B4_3986at 4428.787 kb on + strand, within Pf1N1B4_3986at 4428.788 kb on - strand, within Pf1N1B4_3986at 4428.788 kb on - strand, within Pf1N1B4_3986at 4428.946 kb on + strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.019 kb on - strand, within Pf1N1B4_3986at 4429.223 kb on + strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.291 kb on - strandat 4429.454 kb on - strandat 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.564 kb on + strand, within Pf1N1B4_3987at 4429.630 kb on + strand, within Pf1N1B4_3987at 4429.630 kb on + strand, within Pf1N1B4_3987at 4429.637 kb on - strand, within Pf1N1B4_3987at 4429.663 kb on + strand, within Pf1N1B4_3987at 4429.663 kb on + strand, within Pf1N1B4_3987at 4429.664 kb on - strand, within Pf1N1B4_3987at 4429.664 kb on - strand, within Pf1N1B4_3987at 4429.664 kb on - strandat 4429.738 kb on + strand, within Pf1N1B4_3987at 4429.827 kb on + strand, within Pf1N1B4_3987at 4429.828 kb on - strand, within Pf1N1B4_3987at 4430.023 kb on + strand, within Pf1N1B4_3987at 4430.023 kb on + strand, within Pf1N1B4_3987at 4430.024 kb on - strandat 4430.032 kb on + strand, within Pf1N1B4_3987at 4430.032 kb on + strand, within Pf1N1B4_3987at 4430.033 kb on - strand, within Pf1N1B4_3987at 4430.082 kb on + strand, within Pf1N1B4_3987at 4430.083 kb on - strand, within Pf1N1B4_3987at 4430.086 kb on + strand, within Pf1N1B4_3987at 4430.102 kb on + strand, within Pf1N1B4_3987at 4430.200 kb on + strand, within Pf1N1B4_3987at 4430.201 kb on - strand, within Pf1N1B4_3987at 4430.201 kb on - strand, within Pf1N1B4_3987at 4430.353 kb on + strand, within Pf1N1B4_3987at 4430.537 kb on - strandat 4430.611 kb on + strandat 4430.611 kb on + strandat 4430.671 kb on - strandat 4430.671 kb on - strandat 4430.671 kb on - strandat 4430.753 kb on + strandat 4430.753 kb on + strandat 4430.753 kb on + strandat 4430.757 kb on + strandat 4430.757 kb on + strandat 4430.757 kb on + strandat 4430.757 kb on + strandat 4430.757 kb on + strandat 4430.758 kb on - strandat 4430.758 kb on - strandat 4430.758 kb on - strandat 4430.768 kb on - strandat 4430.847 kb on + strandat 4430.847 kb on + strandat 4430.848 kb on - strandat 4430.852 kb on + strandat 4430.905 kb on + strandat 4430.906 kb on - strandat 4431.220 kb on + strand, within Pf1N1B4_3988at 4431.220 kb on + strand, within Pf1N1B4_3988at 4431.221 kb on - strand, within Pf1N1B4_3988at 4431.221 kb on - strand, within Pf1N1B4_3988at 4431.221 kb on - strand, within Pf1N1B4_3988at 4431.511 kb on + strand, within Pf1N1B4_3988at 4431.512 kb on - strand, within Pf1N1B4_3988

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (C)
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4,428,392 + Pf1N1B4_3986 0.39 +1.1
4,428,783 + Pf1N1B4_3986 0.64 -1.3
4,428,787 + Pf1N1B4_3986 0.64 -1.3
4,428,788 - Pf1N1B4_3986 0.64 +0.3
4,428,788 - Pf1N1B4_3986 0.64 -0.3
4,428,946 + Pf1N1B4_3986 0.74 -1.8
4,428,947 - Pf1N1B4_3986 0.74 -1.8
4,428,947 - Pf1N1B4_3986 0.74 -2.8
4,428,947 - Pf1N1B4_3986 0.74 -1.8
4,429,018 + Pf1N1B4_3986 0.78 -1.6
4,429,018 + Pf1N1B4_3986 0.78 -1.3
4,429,018 + Pf1N1B4_3986 0.78 +0.7
4,429,018 + Pf1N1B4_3986 0.78 +0.5
4,429,019 - Pf1N1B4_3986 0.78 -1.3
4,429,223 + -0.3
4,429,224 - -1.8
4,429,224 - -1.8
4,429,224 - -2.3
4,429,224 - +0.7
4,429,224 - -2.3
4,429,224 - -1.0
4,429,291 - -1.0
4,429,454 - -0.3
4,429,502 - Pf1N1B4_3987 0.10 -0.3
4,429,502 - Pf1N1B4_3987 0.10 -1.5
4,429,502 - Pf1N1B4_3987 0.10 -1.8
4,429,564 + Pf1N1B4_3987 0.16 -1.3
4,429,630 + Pf1N1B4_3987 0.21 -0.7
4,429,630 + Pf1N1B4_3987 0.21 +0.7
4,429,637 - Pf1N1B4_3987 0.22 +0.4
4,429,663 + Pf1N1B4_3987 0.24 +0.7
4,429,663 + Pf1N1B4_3987 0.24 -1.3
4,429,664 - Pf1N1B4_3987 0.24 +0.6
4,429,664 - Pf1N1B4_3987 0.24 -0.3
4,429,664 - -0.3
4,429,738 + Pf1N1B4_3987 0.31 -2.1
4,429,827 + Pf1N1B4_3987 0.38 +0.7
4,429,828 - Pf1N1B4_3987 0.38 -0.7
4,430,023 + Pf1N1B4_3987 0.55 -1.6
4,430,023 + Pf1N1B4_3987 0.55 +0.7
4,430,024 - -0.3
4,430,032 + Pf1N1B4_3987 0.56 +0.7
4,430,032 + Pf1N1B4_3987 0.56 -2.6
4,430,033 - Pf1N1B4_3987 0.56 -0.8
4,430,082 + Pf1N1B4_3987 0.60 +0.7
4,430,083 - Pf1N1B4_3987 0.60 +0.7
4,430,086 + Pf1N1B4_3987 0.61 -1.3
4,430,102 + Pf1N1B4_3987 0.62 -0.8
4,430,200 + Pf1N1B4_3987 0.70 -0.3
4,430,201 - Pf1N1B4_3987 0.71 -1.3
4,430,201 - Pf1N1B4_3987 0.71 -1.3
4,430,353 + Pf1N1B4_3987 0.84 -1.3
4,430,537 - +0.6
4,430,611 + -0.8
4,430,611 + -0.8
4,430,671 - -0.7
4,430,671 - -0.8
4,430,671 - +1.7
4,430,753 + -0.5
4,430,753 + +0.0
4,430,753 + -0.3
4,430,757 + +0.7
4,430,757 + -0.8
4,430,757 + -0.4
4,430,757 + -0.3
4,430,757 + +1.3
4,430,758 - -1.5
4,430,758 - +0.0
4,430,758 - -1.6
4,430,768 - +0.3
4,430,847 + +0.7
4,430,847 + -0.5
4,430,848 - +1.1
4,430,852 + -0.5
4,430,905 + +1.7
4,430,906 - -0.4
4,431,220 + Pf1N1B4_3988 0.20 +0.5
4,431,220 + Pf1N1B4_3988 0.20 -1.8
4,431,221 - Pf1N1B4_3988 0.20 +0.7
4,431,221 - Pf1N1B4_3988 0.20 +2.4
4,431,221 - Pf1N1B4_3988 0.20 +0.9
4,431,511 + Pf1N1B4_3988 0.37 -1.3
4,431,512 - Pf1N1B4_3988 0.37 +0.5

Or see this region's nucleotide sequence