Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3982 and Pf1N1B4_3983 are separated by 120 nucleotides Pf1N1B4_3983 and Pf1N1B4_3984 are separated by 39 nucleotides Pf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotides Pf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides
Pf1N1B4_3982 - FIG00953347: hypothetical protein, at 4,423,698 to 4,424,039
_3982
Pf1N1B4_3983 - cI, at 4,424,160 to 4,424,834
_3983
Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 4,424,874 to 4,426,829
_3984
Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644
_3985
Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367
_3986
Position (kb)
4424
4425
4426
4427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4423.942 kb on + strand, within Pf1N1B4_3982 at 4423.942 kb on + strand, within Pf1N1B4_3982 at 4423.943 kb on - strand, within Pf1N1B4_3982 at 4423.943 kb on - strand at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.946 kb on - strand, within Pf1N1B4_3982 at 4423.946 kb on - strand, within Pf1N1B4_3982 at 4424.060 kb on + strand at 4424.060 kb on + strand at 4424.061 kb on - strand at 4424.085 kb on + strand at 4424.085 kb on + strand at 4424.086 kb on - strand at 4424.088 kb on + strand at 4424.088 kb on + strand at 4424.088 kb on + strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.093 kb on - strand at 4424.093 kb on - strand at 4424.093 kb on - strand at 4424.098 kb on - strand at 4424.121 kb on + strand at 4424.121 kb on + strand at 4424.156 kb on + strand at 4424.231 kb on - strand at 4424.231 kb on - strand, within Pf1N1B4_3983 at 4424.282 kb on + strand, within Pf1N1B4_3983 at 4424.282 kb on + strand at 4424.344 kb on - strand, within Pf1N1B4_3983 at 4424.344 kb on - strand, within Pf1N1B4_3983 at 4424.415 kb on + strand, within Pf1N1B4_3983 at 4424.415 kb on + strand, within Pf1N1B4_3983 at 4424.416 kb on - strand at 4424.482 kb on + strand, within Pf1N1B4_3983 at 4424.538 kb on + strand, within Pf1N1B4_3983 at 4424.807 kb on - strand at 4424.833 kb on - strand at 4424.935 kb on + strand at 4424.936 kb on - strand at 4424.936 kb on - strand at 4425.130 kb on + strand, within Pf1N1B4_3984 at 4425.131 kb on - strand, within Pf1N1B4_3984 at 4425.131 kb on - strand, within Pf1N1B4_3984 at 4425.169 kb on + strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.236 kb on - strand, within Pf1N1B4_3984 at 4425.671 kb on - strand, within Pf1N1B4_3984 at 4426.015 kb on + strand, within Pf1N1B4_3984 at 4426.150 kb on + strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.152 kb on + strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.213 kb on + strand, within Pf1N1B4_3984 at 4426.214 kb on - strand, within Pf1N1B4_3984 at 4426.639 kb on + strand at 4426.640 kb on - strand at 4426.640 kb on - strand at 4427.321 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 4,423,942 + Pf1N1B4_3982 0.71 +0.3 4,423,942 + Pf1N1B4_3982 0.71 +0.3 4,423,943 - Pf1N1B4_3982 0.72 +0.2 4,423,943 - -0.3 4,423,945 + Pf1N1B4_3982 0.72 +0.3 4,423,945 + Pf1N1B4_3982 0.72 -0.5 4,423,945 + Pf1N1B4_3982 0.72 -0.6 4,423,946 - Pf1N1B4_3982 0.73 -0.8 4,423,946 - Pf1N1B4_3982 0.73 -0.5 4,424,060 + -1.5 4,424,060 + +1.7 4,424,061 - +0.7 4,424,085 + +1.2 4,424,085 + -2.6 4,424,086 - -2.3 4,424,088 + +0.1 4,424,088 + -0.3 4,424,088 + +2.3 4,424,089 - +1.1 4,424,089 - -0.0 4,424,089 - +1.9 4,424,089 - +0.1 4,424,092 + +0.2 4,424,092 + -0.2 4,424,092 + +0.7 4,424,092 + +0.9 4,424,092 + -1.8 4,424,092 + +2.1 4,424,092 + -0.8 4,424,092 + +0.2 4,424,093 - -0.3 4,424,093 - +0.7 4,424,093 - -0.1 4,424,098 - -1.3 4,424,121 + +0.3 4,424,121 + +0.7 4,424,156 + +0.7 4,424,231 - +1.3 4,424,231 - Pf1N1B4_3983 0.11 +1.2 4,424,282 + Pf1N1B4_3983 0.18 -0.3 4,424,282 + -1.8 4,424,344 - Pf1N1B4_3983 0.27 +0.7 4,424,344 - Pf1N1B4_3983 0.27 -1.3 4,424,415 + Pf1N1B4_3983 0.38 +0.3 4,424,415 + Pf1N1B4_3983 0.38 -0.3 4,424,416 - -0.3 4,424,482 + Pf1N1B4_3983 0.48 +0.2 4,424,538 + Pf1N1B4_3983 0.56 -0.0 4,424,807 - +0.5 4,424,833 - -0.7 4,424,935 + -0.3 4,424,936 - -1.3 4,424,936 - -1.3 4,425,130 + Pf1N1B4_3984 0.13 -1.6 4,425,131 - Pf1N1B4_3984 0.13 +0.3 4,425,131 - Pf1N1B4_3984 0.13 -1.8 4,425,169 + Pf1N1B4_3984 0.15 -1.3 4,425,170 - Pf1N1B4_3984 0.15 -1.3 4,425,170 - Pf1N1B4_3984 0.15 -0.7 4,425,170 - Pf1N1B4_3984 0.15 -0.3 4,425,236 - Pf1N1B4_3984 0.19 -2.3 4,425,671 - Pf1N1B4_3984 0.41 -0.3 4,426,015 + Pf1N1B4_3984 0.58 -0.8 4,426,150 + Pf1N1B4_3984 0.65 -1.3 4,426,151 - Pf1N1B4_3984 0.65 -2.6 4,426,151 - Pf1N1B4_3984 0.65 +1.7 4,426,151 - Pf1N1B4_3984 0.65 -1.8 4,426,151 - Pf1N1B4_3984 0.65 -2.6 4,426,151 - Pf1N1B4_3984 0.65 +0.7 4,426,152 + Pf1N1B4_3984 0.65 -0.8 4,426,153 - Pf1N1B4_3984 0.65 +0.7 4,426,153 - Pf1N1B4_3984 0.65 -2.1 4,426,213 + Pf1N1B4_3984 0.68 -2.8 4,426,214 - Pf1N1B4_3984 0.69 -0.3 4,426,639 + -0.7 4,426,640 - -1.8 4,426,640 - -1.3 4,427,321 + Pf1N1B4_3985 0.60 -1.8 4,427,361 + Pf1N1B4_3985 0.65 -1.8 4,427,361 + Pf1N1B4_3985 0.65 -1.3 4,427,361 + Pf1N1B4_3985 0.65 -0.8
Or see this region's nucleotide sequence