Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2505

Experiment: L-Valine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2503 and Pf1N1B4_2504 are separated by 38 nucleotidesPf1N1B4_2504 and Pf1N1B4_2505 are separated by 12 nucleotidesPf1N1B4_2505 and Pf1N1B4_2506 are separated by 215 nucleotidesPf1N1B4_2506 and Pf1N1B4_2507 are separated by 117 nucleotides Pf1N1B4_2503 - Coenzyme PQQ synthesis protein F, at 2,838,560 to 2,840,998 _2503 Pf1N1B4_2504 - 5-aminopentanamidase (EC 3.5.1.30) (from data), at 2,841,037 to 2,841,831 _2504 Pf1N1B4_2505 - Lysine 2-monooxygenase (EC 1.13.12.2) (from data), at 2,841,844 to 2,843,526 _2505 Pf1N1B4_2506 - Transcriptional regulator, AsnC family, at 2,843,742 to 2,844,203 _2506 Pf1N1B4_2507 - hypothetical protein, at 2,844,321 to 2,844,482 _2507 Position (kb) 2841 2842 2843 2844Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2841.025 kb on - strandat 2841.060 kb on - strandat 2841.061 kb on + strandat 2841.062 kb on - strandat 2841.062 kb on - strandat 2841.062 kb on - strandat 2841.062 kb on - strandat 2841.087 kb on - strandat 2841.212 kb on + strand, within Pf1N1B4_2504at 2841.239 kb on + strand, within Pf1N1B4_2504at 2841.239 kb on + strand, within Pf1N1B4_2504at 2841.240 kb on - strand, within Pf1N1B4_2504at 2841.240 kb on - strand, within Pf1N1B4_2504at 2841.240 kb on - strand, within Pf1N1B4_2504at 2841.240 kb on - strand, within Pf1N1B4_2504at 2841.248 kb on + strand, within Pf1N1B4_2504at 2841.249 kb on - strand, within Pf1N1B4_2504at 2841.257 kb on + strand, within Pf1N1B4_2504at 2841.257 kb on + strand, within Pf1N1B4_2504at 2841.258 kb on - strandat 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.258 kb on - strand, within Pf1N1B4_2504at 2841.509 kb on + strand, within Pf1N1B4_2504at 2841.548 kb on - strand, within Pf1N1B4_2504at 2841.557 kb on + strand, within Pf1N1B4_2504at 2841.557 kb on + strand, within Pf1N1B4_2504at 2841.557 kb on + strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.558 kb on - strand, within Pf1N1B4_2504at 2841.593 kb on + strand, within Pf1N1B4_2504at 2841.594 kb on - strand, within Pf1N1B4_2504at 2841.594 kb on - strand, within Pf1N1B4_2504at 2841.699 kb on - strand, within Pf1N1B4_2504at 2841.699 kb on - strand, within Pf1N1B4_2504at 2841.781 kb on - strandat 2841.781 kb on - strandat 2841.845 kb on + strandat 2841.845 kb on + strandat 2842.064 kb on + strand, within Pf1N1B4_2505at 2842.079 kb on + strand, within Pf1N1B4_2505at 2842.280 kb on + strand, within Pf1N1B4_2505at 2842.280 kb on + strand, within Pf1N1B4_2505at 2842.281 kb on - strand, within Pf1N1B4_2505at 2842.329 kb on - strand, within Pf1N1B4_2505at 2842.329 kb on - strand, within Pf1N1B4_2505at 2842.329 kb on - strand, within Pf1N1B4_2505at 2842.448 kb on + strand, within Pf1N1B4_2505at 2842.449 kb on - strand, within Pf1N1B4_2505at 2842.547 kb on - strand, within Pf1N1B4_2505at 2842.574 kb on + strandat 2842.575 kb on - strand, within Pf1N1B4_2505at 2842.575 kb on - strand, within Pf1N1B4_2505at 2842.591 kb on - strand, within Pf1N1B4_2505at 2842.591 kb on - strand, within Pf1N1B4_2505at 2842.591 kb on - strand, within Pf1N1B4_2505at 2842.591 kb on - strand, within Pf1N1B4_2505at 2842.744 kb on + strand, within Pf1N1B4_2505at 2842.913 kb on + strand, within Pf1N1B4_2505at 2842.913 kb on + strand, within Pf1N1B4_2505at 2842.914 kb on - strand, within Pf1N1B4_2505at 2843.103 kb on + strand, within Pf1N1B4_2505at 2843.104 kb on - strand, within Pf1N1B4_2505at 2843.189 kb on + strand, within Pf1N1B4_2505at 2843.189 kb on + strand, within Pf1N1B4_2505at 2843.189 kb on + strand, within Pf1N1B4_2505at 2843.196 kb on - strand, within Pf1N1B4_2505at 2843.226 kb on + strand, within Pf1N1B4_2505at 2843.306 kb on + strand, within Pf1N1B4_2505at 2843.307 kb on - strand, within Pf1N1B4_2505at 2843.350 kb on + strand, within Pf1N1B4_2505at 2843.413 kb on - strandat 2843.494 kb on - strandat 2843.570 kb on - strandat 2843.571 kb on + strandat 2843.572 kb on - strandat 2843.572 kb on - strandat 2843.572 kb on - strandat 2843.572 kb on - strandat 2843.594 kb on - strandat 2843.603 kb on - strandat 2843.616 kb on - strandat 2843.616 kb on - strandat 2843.616 kb on - strandat 2843.616 kb on - strandat 2843.672 kb on + strandat 2843.677 kb on - strandat 2843.677 kb on - strandat 2843.729 kb on + strandat 2843.878 kb on + strand, within Pf1N1B4_2506at 2843.878 kb on + strand, within Pf1N1B4_2506at 2843.878 kb on + strand, within Pf1N1B4_2506at 2843.879 kb on - strand, within Pf1N1B4_2506at 2843.983 kb on + strand, within Pf1N1B4_2506at 2843.983 kb on + strand, within Pf1N1B4_2506at 2843.983 kb on + strand, within Pf1N1B4_2506at 2843.983 kb on + strand, within Pf1N1B4_2506at 2843.983 kb on + strand, within Pf1N1B4_2506at 2843.984 kb on - strandat 2843.984 kb on - strand, within Pf1N1B4_2506at 2843.984 kb on - strand, within Pf1N1B4_2506at 2843.984 kb on - strand, within Pf1N1B4_2506at 2843.984 kb on - strand, within Pf1N1B4_2506at 2844.244 kb on + strandat 2844.244 kb on + strandat 2844.245 kb on - strandat 2844.245 kb on - strandat 2844.299 kb on + strandat 2844.300 kb on - strandat 2844.322 kb on + strandat 2844.322 kb on + strandat 2844.322 kb on + strandat 2844.372 kb on + strand, within Pf1N1B4_2507at 2844.373 kb on - strand, within Pf1N1B4_2507at 2844.373 kb on - strand, within Pf1N1B4_2507at 2844.408 kb on + strand, within Pf1N1B4_2507at 2844.408 kb on + strand, within Pf1N1B4_2507at 2844.409 kb on - strand, within Pf1N1B4_2507at 2844.409 kb on - strand, within Pf1N1B4_2507at 2844.415 kb on + strandat 2844.497 kb on + strandat 2844.497 kb on + strandat 2844.497 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (C)
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2,841,025 - +0.2
2,841,060 - +0.6
2,841,061 + +0.2
2,841,062 - -0.5
2,841,062 - -0.8
2,841,062 - +1.1
2,841,062 - +0.2
2,841,087 - +1.4
2,841,212 + Pf1N1B4_2504 0.22 +0.6
2,841,239 + Pf1N1B4_2504 0.25 +1.0
2,841,239 + Pf1N1B4_2504 0.25 +2.1
2,841,240 - Pf1N1B4_2504 0.26 +1.8
2,841,240 - Pf1N1B4_2504 0.26 -0.2
2,841,240 - Pf1N1B4_2504 0.26 -0.3
2,841,240 - Pf1N1B4_2504 0.26 +1.6
2,841,248 + Pf1N1B4_2504 0.27 +1.1
2,841,249 - Pf1N1B4_2504 0.27 -0.4
2,841,257 + Pf1N1B4_2504 0.28 +0.2
2,841,257 + Pf1N1B4_2504 0.28 -0.8
2,841,258 - +1.8
2,841,258 - Pf1N1B4_2504 0.28 +1.7
2,841,258 - Pf1N1B4_2504 0.28 +0.2
2,841,258 - Pf1N1B4_2504 0.28 -0.8
2,841,258 - Pf1N1B4_2504 0.28 -0.8
2,841,258 - Pf1N1B4_2504 0.28 +2.8
2,841,258 - Pf1N1B4_2504 0.28 +0.8
2,841,258 - Pf1N1B4_2504 0.28 -1.0
2,841,509 + Pf1N1B4_2504 0.59 +0.2
2,841,548 - Pf1N1B4_2504 0.64 +0.1
2,841,557 + Pf1N1B4_2504 0.65 +1.2
2,841,557 + Pf1N1B4_2504 0.65 +0.6
2,841,557 + Pf1N1B4_2504 0.65 +1.0
2,841,558 - Pf1N1B4_2504 0.66 +1.8
2,841,558 - Pf1N1B4_2504 0.66 -0.1
2,841,558 - Pf1N1B4_2504 0.66 +0.8
2,841,558 - Pf1N1B4_2504 0.66 +0.8
2,841,558 - Pf1N1B4_2504 0.66 +1.1
2,841,558 - Pf1N1B4_2504 0.66 -1.8
2,841,558 - Pf1N1B4_2504 0.66 -0.8
2,841,558 - Pf1N1B4_2504 0.66 +0.8
2,841,593 + Pf1N1B4_2504 0.70 -1.6
2,841,594 - Pf1N1B4_2504 0.70 -1.0
2,841,594 - Pf1N1B4_2504 0.70 +0.9
2,841,699 - Pf1N1B4_2504 0.83 +1.2
2,841,699 - Pf1N1B4_2504 0.83 +0.2
2,841,781 - -0.3
2,841,781 - +1.2
2,841,845 + +0.5
2,841,845 + +1.8
2,842,064 + Pf1N1B4_2505 0.13 +0.2
2,842,079 + Pf1N1B4_2505 0.14 +2.8
2,842,280 + Pf1N1B4_2505 0.26 -0.3
2,842,280 + Pf1N1B4_2505 0.26 +0.8
2,842,281 - Pf1N1B4_2505 0.26 +0.2
2,842,329 - Pf1N1B4_2505 0.29 +2.1
2,842,329 - Pf1N1B4_2505 0.29 +1.0
2,842,329 - Pf1N1B4_2505 0.29 +2.7
2,842,448 + Pf1N1B4_2505 0.36 +0.2
2,842,449 - Pf1N1B4_2505 0.36 +0.2
2,842,547 - Pf1N1B4_2505 0.42 +0.6
2,842,574 + +0.2
2,842,575 - Pf1N1B4_2505 0.43 +0.8
2,842,575 - Pf1N1B4_2505 0.43 +0.8
2,842,591 - Pf1N1B4_2505 0.44 -0.8
2,842,591 - Pf1N1B4_2505 0.44 +3.1
2,842,591 - Pf1N1B4_2505 0.44 +1.8
2,842,591 - Pf1N1B4_2505 0.44 +0.8
2,842,744 + Pf1N1B4_2505 0.53 +0.2
2,842,913 + Pf1N1B4_2505 0.64 +0.8
2,842,913 + Pf1N1B4_2505 0.64 +0.8
2,842,914 - Pf1N1B4_2505 0.64 -0.2
2,843,103 + Pf1N1B4_2505 0.75 +1.1
2,843,104 - Pf1N1B4_2505 0.75 +0.5
2,843,189 + Pf1N1B4_2505 0.80 +0.8
2,843,189 + Pf1N1B4_2505 0.80 -0.8
2,843,189 + Pf1N1B4_2505 0.80 +3.4
2,843,196 - Pf1N1B4_2505 0.80 -0.5
2,843,226 + Pf1N1B4_2505 0.82 +1.1
2,843,306 + Pf1N1B4_2505 0.87 +1.8
2,843,307 - Pf1N1B4_2505 0.87 -0.8
2,843,350 + Pf1N1B4_2505 0.89 +1.2
2,843,413 - +0.6
2,843,494 - +1.2
2,843,570 - +1.2
2,843,571 + +2.2
2,843,572 - +0.6
2,843,572 - -1.2
2,843,572 - -0.3
2,843,572 - +0.2
2,843,594 - -1.3
2,843,603 - -1.8
2,843,616 - +1.2
2,843,616 - -1.3
2,843,616 - +1.8
2,843,616 - -0.1
2,843,672 + +0.7
2,843,677 - -0.8
2,843,677 - +0.6
2,843,729 + +0.9
2,843,878 + Pf1N1B4_2506 0.29 +0.8
2,843,878 + Pf1N1B4_2506 0.29 +0.6
2,843,878 + Pf1N1B4_2506 0.29 +1.2
2,843,879 - Pf1N1B4_2506 0.30 -0.5
2,843,983 + Pf1N1B4_2506 0.52 +1.6
2,843,983 + Pf1N1B4_2506 0.52 +1.3
2,843,983 + Pf1N1B4_2506 0.52 +1.2
2,843,983 + Pf1N1B4_2506 0.52 -1.1
2,843,983 + Pf1N1B4_2506 0.52 +1.6
2,843,984 - +1.2
2,843,984 - Pf1N1B4_2506 0.52 -0.3
2,843,984 - Pf1N1B4_2506 0.52 +1.1
2,843,984 - Pf1N1B4_2506 0.52 +1.1
2,843,984 - Pf1N1B4_2506 0.52 -0.8
2,844,244 + -0.5
2,844,244 + -2.1
2,844,245 - +0.7
2,844,245 - +0.6
2,844,299 + -0.8
2,844,300 - -0.2
2,844,322 + -1.2
2,844,322 + -0.4
2,844,322 + -1.0
2,844,372 + Pf1N1B4_2507 0.31 -1.3
2,844,373 - Pf1N1B4_2507 0.32 -0.2
2,844,373 - Pf1N1B4_2507 0.32 +0.5
2,844,408 + Pf1N1B4_2507 0.54 -0.9
2,844,408 + Pf1N1B4_2507 0.54 -0.6
2,844,409 - Pf1N1B4_2507 0.54 +1.2
2,844,409 - Pf1N1B4_2507 0.54 +1.2
2,844,415 + +0.7
2,844,497 + -0.3
2,844,497 + +0.5
2,844,497 + +0.8

Or see this region's nucleotide sequence