Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2503 and Pf1N1B4_2504 are separated by 38 nucleotides Pf1N1B4_2504 and Pf1N1B4_2505 are separated by 12 nucleotides Pf1N1B4_2505 and Pf1N1B4_2506 are separated by 215 nucleotides Pf1N1B4_2506 and Pf1N1B4_2507 are separated by 117 nucleotides
Pf1N1B4_2503 - Coenzyme PQQ synthesis protein F, at 2,838,560 to 2,840,998
_2503
Pf1N1B4_2504 - 5-aminopentanamidase (EC 3.5.1.30) (from data) , at 2,841,037 to 2,841,831
_2504
Pf1N1B4_2505 - Lysine 2-monooxygenase (EC 1.13.12.2) (from data) , at 2,841,844 to 2,843,526
_2505
Pf1N1B4_2506 - Transcriptional regulator, AsnC family, at 2,843,742 to 2,844,203
_2506
Pf1N1B4_2507 - hypothetical protein, at 2,844,321 to 2,844,482
_2507
Position (kb)
2841
2842
2843
2844 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2841.025 kb on - strand at 2841.060 kb on - strand at 2841.061 kb on + strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.087 kb on - strand at 2841.212 kb on + strand, within Pf1N1B4_2504 at 2841.239 kb on + strand, within Pf1N1B4_2504 at 2841.239 kb on + strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.248 kb on + strand, within Pf1N1B4_2504 at 2841.249 kb on - strand, within Pf1N1B4_2504 at 2841.257 kb on + strand, within Pf1N1B4_2504 at 2841.257 kb on + strand, within Pf1N1B4_2504 at 2841.258 kb on - strand at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.509 kb on + strand, within Pf1N1B4_2504 at 2841.548 kb on - strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.593 kb on + strand, within Pf1N1B4_2504 at 2841.594 kb on - strand, within Pf1N1B4_2504 at 2841.594 kb on - strand, within Pf1N1B4_2504 at 2841.699 kb on - strand, within Pf1N1B4_2504 at 2841.699 kb on - strand, within Pf1N1B4_2504 at 2841.781 kb on - strand at 2841.781 kb on - strand at 2841.845 kb on + strand at 2841.845 kb on + strand at 2842.064 kb on + strand, within Pf1N1B4_2505 at 2842.079 kb on + strand, within Pf1N1B4_2505 at 2842.280 kb on + strand, within Pf1N1B4_2505 at 2842.280 kb on + strand, within Pf1N1B4_2505 at 2842.281 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.448 kb on + strand, within Pf1N1B4_2505 at 2842.449 kb on - strand, within Pf1N1B4_2505 at 2842.547 kb on - strand, within Pf1N1B4_2505 at 2842.574 kb on + strand at 2842.575 kb on - strand, within Pf1N1B4_2505 at 2842.575 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.744 kb on + strand, within Pf1N1B4_2505 at 2842.913 kb on + strand, within Pf1N1B4_2505 at 2842.913 kb on + strand, within Pf1N1B4_2505 at 2842.914 kb on - strand, within Pf1N1B4_2505 at 2843.103 kb on + strand, within Pf1N1B4_2505 at 2843.104 kb on - strand, within Pf1N1B4_2505 at 2843.189 kb on + strand, within Pf1N1B4_2505 at 2843.189 kb on + strand, within Pf1N1B4_2505 at 2843.189 kb on + strand, within Pf1N1B4_2505 at 2843.196 kb on - strand, within Pf1N1B4_2505 at 2843.226 kb on + strand, within Pf1N1B4_2505 at 2843.306 kb on + strand, within Pf1N1B4_2505 at 2843.307 kb on - strand, within Pf1N1B4_2505 at 2843.350 kb on + strand, within Pf1N1B4_2505 at 2843.413 kb on - strand at 2843.494 kb on - strand at 2843.570 kb on - strand at 2843.571 kb on + strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.594 kb on - strand at 2843.603 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.672 kb on + strand at 2843.677 kb on - strand at 2843.677 kb on - strand at 2843.729 kb on + strand at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.879 kb on - strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.984 kb on - strand at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2844.244 kb on + strand at 2844.244 kb on + strand at 2844.245 kb on - strand at 2844.245 kb on - strand at 2844.299 kb on + strand at 2844.300 kb on - strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.372 kb on + strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.415 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 2,841,025 - +0.2 2,841,060 - +0.6 2,841,061 + +0.2 2,841,062 - -0.5 2,841,062 - -0.8 2,841,062 - +1.1 2,841,062 - +0.2 2,841,087 - +1.4 2,841,212 + Pf1N1B4_2504 0.22 +0.6 2,841,239 + Pf1N1B4_2504 0.25 +1.0 2,841,239 + Pf1N1B4_2504 0.25 +2.1 2,841,240 - Pf1N1B4_2504 0.26 +1.8 2,841,240 - Pf1N1B4_2504 0.26 -0.2 2,841,240 - Pf1N1B4_2504 0.26 -0.3 2,841,240 - Pf1N1B4_2504 0.26 +1.6 2,841,248 + Pf1N1B4_2504 0.27 +1.1 2,841,249 - Pf1N1B4_2504 0.27 -0.4 2,841,257 + Pf1N1B4_2504 0.28 +0.2 2,841,257 + Pf1N1B4_2504 0.28 -0.8 2,841,258 - +1.8 2,841,258 - Pf1N1B4_2504 0.28 +1.7 2,841,258 - Pf1N1B4_2504 0.28 +0.2 2,841,258 - Pf1N1B4_2504 0.28 -0.8 2,841,258 - Pf1N1B4_2504 0.28 -0.8 2,841,258 - Pf1N1B4_2504 0.28 +2.8 2,841,258 - Pf1N1B4_2504 0.28 +0.8 2,841,258 - Pf1N1B4_2504 0.28 -1.0 2,841,509 + Pf1N1B4_2504 0.59 +0.2 2,841,548 - Pf1N1B4_2504 0.64 +0.1 2,841,557 + Pf1N1B4_2504 0.65 +1.2 2,841,557 + Pf1N1B4_2504 0.65 +0.6 2,841,557 + Pf1N1B4_2504 0.65 +1.0 2,841,558 - Pf1N1B4_2504 0.66 +1.8 2,841,558 - Pf1N1B4_2504 0.66 -0.1 2,841,558 - Pf1N1B4_2504 0.66 +0.8 2,841,558 - Pf1N1B4_2504 0.66 +0.8 2,841,558 - Pf1N1B4_2504 0.66 +1.1 2,841,558 - Pf1N1B4_2504 0.66 -1.8 2,841,558 - Pf1N1B4_2504 0.66 -0.8 2,841,558 - Pf1N1B4_2504 0.66 +0.8 2,841,593 + Pf1N1B4_2504 0.70 -1.6 2,841,594 - Pf1N1B4_2504 0.70 -1.0 2,841,594 - Pf1N1B4_2504 0.70 +0.9 2,841,699 - Pf1N1B4_2504 0.83 +1.2 2,841,699 - Pf1N1B4_2504 0.83 +0.2 2,841,781 - -0.3 2,841,781 - +1.2 2,841,845 + +0.5 2,841,845 + +1.8 2,842,064 + Pf1N1B4_2505 0.13 +0.2 2,842,079 + Pf1N1B4_2505 0.14 +2.8 2,842,280 + Pf1N1B4_2505 0.26 -0.3 2,842,280 + Pf1N1B4_2505 0.26 +0.8 2,842,281 - Pf1N1B4_2505 0.26 +0.2 2,842,329 - Pf1N1B4_2505 0.29 +2.1 2,842,329 - Pf1N1B4_2505 0.29 +1.0 2,842,329 - Pf1N1B4_2505 0.29 +2.7 2,842,448 + Pf1N1B4_2505 0.36 +0.2 2,842,449 - Pf1N1B4_2505 0.36 +0.2 2,842,547 - Pf1N1B4_2505 0.42 +0.6 2,842,574 + +0.2 2,842,575 - Pf1N1B4_2505 0.43 +0.8 2,842,575 - Pf1N1B4_2505 0.43 +0.8 2,842,591 - Pf1N1B4_2505 0.44 -0.8 2,842,591 - Pf1N1B4_2505 0.44 +3.1 2,842,591 - Pf1N1B4_2505 0.44 +1.8 2,842,591 - Pf1N1B4_2505 0.44 +0.8 2,842,744 + Pf1N1B4_2505 0.53 +0.2 2,842,913 + Pf1N1B4_2505 0.64 +0.8 2,842,913 + Pf1N1B4_2505 0.64 +0.8 2,842,914 - Pf1N1B4_2505 0.64 -0.2 2,843,103 + Pf1N1B4_2505 0.75 +1.1 2,843,104 - Pf1N1B4_2505 0.75 +0.5 2,843,189 + Pf1N1B4_2505 0.80 +0.8 2,843,189 + Pf1N1B4_2505 0.80 -0.8 2,843,189 + Pf1N1B4_2505 0.80 +3.4 2,843,196 - Pf1N1B4_2505 0.80 -0.5 2,843,226 + Pf1N1B4_2505 0.82 +1.1 2,843,306 + Pf1N1B4_2505 0.87 +1.8 2,843,307 - Pf1N1B4_2505 0.87 -0.8 2,843,350 + Pf1N1B4_2505 0.89 +1.2 2,843,413 - +0.6 2,843,494 - +1.2 2,843,570 - +1.2 2,843,571 + +2.2 2,843,572 - +0.6 2,843,572 - -1.2 2,843,572 - -0.3 2,843,572 - +0.2 2,843,594 - -1.3 2,843,603 - -1.8 2,843,616 - +1.2 2,843,616 - -1.3 2,843,616 - +1.8 2,843,616 - -0.1 2,843,672 + +0.7 2,843,677 - -0.8 2,843,677 - +0.6 2,843,729 + +0.9 2,843,878 + Pf1N1B4_2506 0.29 +0.8 2,843,878 + Pf1N1B4_2506 0.29 +0.6 2,843,878 + Pf1N1B4_2506 0.29 +1.2 2,843,879 - Pf1N1B4_2506 0.30 -0.5 2,843,983 + Pf1N1B4_2506 0.52 +1.6 2,843,983 + Pf1N1B4_2506 0.52 +1.3 2,843,983 + Pf1N1B4_2506 0.52 +1.2 2,843,983 + Pf1N1B4_2506 0.52 -1.1 2,843,983 + Pf1N1B4_2506 0.52 +1.6 2,843,984 - +1.2 2,843,984 - Pf1N1B4_2506 0.52 -0.3 2,843,984 - Pf1N1B4_2506 0.52 +1.1 2,843,984 - Pf1N1B4_2506 0.52 +1.1 2,843,984 - Pf1N1B4_2506 0.52 -0.8 2,844,244 + -0.5 2,844,244 + -2.1 2,844,245 - +0.7 2,844,245 - +0.6 2,844,299 + -0.8 2,844,300 - -0.2 2,844,322 + -1.2 2,844,322 + -0.4 2,844,322 + -1.0 2,844,372 + Pf1N1B4_2507 0.31 -1.3 2,844,373 - Pf1N1B4_2507 0.32 -0.2 2,844,373 - Pf1N1B4_2507 0.32 +0.5 2,844,408 + Pf1N1B4_2507 0.54 -0.9 2,844,408 + Pf1N1B4_2507 0.54 -0.6 2,844,409 - Pf1N1B4_2507 0.54 +1.2 2,844,409 - Pf1N1B4_2507 0.54 +1.2 2,844,415 + +0.7 2,844,497 + -0.3 2,844,497 + +0.5 2,844,497 + +0.8
Or see this region's nucleotide sequence