Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1974 and Pf1N1B4_1975 are separated by 146 nucleotides Pf1N1B4_1975 and Pf1N1B4_1976 are separated by 122 nucleotides Pf1N1B4_1976 and Pf1N1B4_1977 are separated by 190 nucleotides
Pf1N1B4_1974 - L-lysine permease, at 2,257,448 to 2,258,077
_1974
Pf1N1B4_1975 - Transcriptional regulator, AsnC family, at 2,258,224 to 2,258,697
_1975
Pf1N1B4_1976 - Dihydrolipoamide dehydrogenase (EC 1.8.1.4), at 2,258,820 to 2,260,220
_1976
Pf1N1B4_1977 - Membrane protease subunits, stomatin/prohibitin homologs, at 2,260,411 to 2,262,396
_1977
Position (kb)
2258
2259
2260
2261 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2257.941 kb on - strand, within Pf1N1B4_1974 at 2258.034 kb on + strand at 2258.034 kb on + strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.035 kb on - strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.117 kb on + strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.118 kb on - strand at 2258.179 kb on - strand at 2258.575 kb on + strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.576 kb on - strand, within Pf1N1B4_1975 at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.695 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.821 kb on + strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2258.822 kb on - strand at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand, within Pf1N1B4_1976 at 2259.024 kb on + strand at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.025 kb on - strand, within Pf1N1B4_1976 at 2259.098 kb on + strand, within Pf1N1B4_1976 at 2259.098 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.100 kb on + strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.101 kb on - strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.166 kb on + strand, within Pf1N1B4_1976 at 2259.167 kb on - strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.190 kb on + strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.191 kb on - strand, within Pf1N1B4_1976 at 2259.389 kb on + strand, within Pf1N1B4_1976 at 2259.389 kb on + strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.390 kb on - strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.391 kb on + strand, within Pf1N1B4_1976 at 2259.392 kb on - strand, within Pf1N1B4_1976 at 2259.421 kb on + strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2259.422 kb on - strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.042 kb on + strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.043 kb on - strand, within Pf1N1B4_1976 at 2260.172 kb on + strand at 2260.173 kb on - strand at 2260.173 kb on - strand at 2260.173 kb on - strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.210 kb on + strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.211 kb on - strand at 2260.229 kb on + strand at 2260.229 kb on + strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.230 kb on - strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.263 kb on + strand at 2260.264 kb on - strand at 2260.264 kb on - strand at 2260.280 kb on + strand at 2260.281 kb on - strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.288 kb on + strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.289 kb on - strand at 2260.291 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.350 kb on + strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.351 kb on - strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.354 kb on + strand at 2260.355 kb on - strand at 2260.355 kb on - strand at 2260.364 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.373 kb on + strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.374 kb on - strand at 2260.432 kb on - strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.466 kb on + strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.467 kb on - strand at 2260.475 kb on + strand at 2260.762 kb on + strand, within Pf1N1B4_1977 at 2260.762 kb on + strand, within Pf1N1B4_1977 at 2260.832 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.864 kb on + strand, within Pf1N1B4_1977 at 2260.865 kb on - strand, within Pf1N1B4_1977 at 2260.865 kb on - strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.084 kb on + strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977 at 2261.085 kb on - strand, within Pf1N1B4_1977
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 2,257,941 - Pf1N1B4_1974 0.78 +0.6 2,258,034 + -0.7 2,258,034 + +0.7 2,258,035 - -0.5 2,258,035 - +0.3 2,258,035 - -0.0 2,258,035 - +0.1 2,258,117 + -1.3 2,258,117 + -0.3 2,258,117 + +1.3 2,258,117 + +0.3 2,258,118 - -0.1 2,258,118 - +0.9 2,258,118 - +0.3 2,258,118 - -0.7 2,258,118 - +0.2 2,258,179 - -0.1 2,258,575 + Pf1N1B4_1975 0.74 -0.8 2,258,576 - Pf1N1B4_1975 0.74 -0.7 2,258,576 - Pf1N1B4_1975 0.74 +0.3 2,258,576 - Pf1N1B4_1975 0.74 +0.3 2,258,695 + +0.9 2,258,695 + +0.9 2,258,695 + +0.3 2,258,821 + +0.3 2,258,821 + +0.3 2,258,821 + +0.3 2,258,821 + +0.3 2,258,821 + -0.7 2,258,821 + -0.4 2,258,821 + +0.6 2,258,821 + +0.1 2,258,822 - +0.3 2,258,822 - +0.5 2,258,822 - +1.3 2,258,822 - +0.9 2,258,822 - +1.3 2,258,822 - -1.1 2,258,822 - +2.9 2,258,822 - -1.3 2,259,024 + Pf1N1B4_1976 0.15 +0.3 2,259,024 + Pf1N1B4_1976 0.15 +1.0 2,259,024 + Pf1N1B4_1976 0.15 +0.5 2,259,024 + -0.7 2,259,025 - Pf1N1B4_1976 0.15 +0.3 2,259,025 - Pf1N1B4_1976 0.15 +0.3 2,259,025 - Pf1N1B4_1976 0.15 +0.3 2,259,025 - Pf1N1B4_1976 0.15 -1.7 2,259,025 - Pf1N1B4_1976 0.15 +1.0 2,259,025 - Pf1N1B4_1976 0.15 -0.4 2,259,098 + Pf1N1B4_1976 0.20 +0.3 2,259,098 + Pf1N1B4_1976 0.20 +0.9 2,259,100 + Pf1N1B4_1976 0.20 +0.6 2,259,100 + Pf1N1B4_1976 0.20 -0.7 2,259,100 + Pf1N1B4_1976 0.20 +1.9 2,259,100 + Pf1N1B4_1976 0.20 -0.7 2,259,101 - Pf1N1B4_1976 0.20 +1.9 2,259,101 - Pf1N1B4_1976 0.20 +0.3 2,259,101 - Pf1N1B4_1976 0.20 -1.3 2,259,166 + Pf1N1B4_1976 0.25 -0.1 2,259,166 + Pf1N1B4_1976 0.25 -1.7 2,259,166 + Pf1N1B4_1976 0.25 -0.7 2,259,166 + Pf1N1B4_1976 0.25 +0.3 2,259,167 - Pf1N1B4_1976 0.25 +0.9 2,259,190 + Pf1N1B4_1976 0.26 +2.9 2,259,190 + Pf1N1B4_1976 0.26 +0.8 2,259,190 + Pf1N1B4_1976 0.26 +0.8 2,259,190 + Pf1N1B4_1976 0.26 +1.1 2,259,191 - Pf1N1B4_1976 0.26 +0.3 2,259,191 - Pf1N1B4_1976 0.26 -0.2 2,259,191 - Pf1N1B4_1976 0.26 -0.3 2,259,191 - Pf1N1B4_1976 0.26 +0.6 2,259,191 - Pf1N1B4_1976 0.26 +0.5 2,259,191 - Pf1N1B4_1976 0.26 +1.1 2,259,389 + Pf1N1B4_1976 0.41 -0.2 2,259,389 + Pf1N1B4_1976 0.41 -0.4 2,259,390 - Pf1N1B4_1976 0.41 -0.9 2,259,390 - Pf1N1B4_1976 0.41 -0.4 2,259,390 - Pf1N1B4_1976 0.41 +0.1 2,259,390 - Pf1N1B4_1976 0.41 -1.3 2,259,391 + Pf1N1B4_1976 0.41 -0.4 2,259,391 + Pf1N1B4_1976 0.41 +0.1 2,259,391 + Pf1N1B4_1976 0.41 -0.3 2,259,391 + Pf1N1B4_1976 0.41 +1.2 2,259,391 + Pf1N1B4_1976 0.41 +1.3 2,259,392 - Pf1N1B4_1976 0.41 +0.4 2,259,421 + Pf1N1B4_1976 0.43 +0.7 2,259,422 - Pf1N1B4_1976 0.43 -0.4 2,259,422 - Pf1N1B4_1976 0.43 -0.7 2,259,422 - Pf1N1B4_1976 0.43 -1.5 2,260,042 + Pf1N1B4_1976 0.87 -1.1 2,260,042 + Pf1N1B4_1976 0.87 +0.2 2,260,042 + Pf1N1B4_1976 0.87 -0.2 2,260,043 - Pf1N1B4_1976 0.87 -0.3 2,260,043 - Pf1N1B4_1976 0.87 +1.3 2,260,043 - Pf1N1B4_1976 0.87 +0.8 2,260,172 + -1.1 2,260,173 - -1.1 2,260,173 - -0.9 2,260,173 - -0.1 2,260,210 + -0.0 2,260,210 + -1.0 2,260,210 + -0.4 2,260,210 + -0.3 2,260,210 + -0.6 2,260,210 + +0.3 2,260,210 + -0.7 2,260,210 + -0.8 2,260,210 + +0.3 2,260,210 + -0.2 2,260,210 + +2.3 2,260,210 + +0.1 2,260,210 + -0.7 2,260,210 + +3.8 2,260,211 - +1.5 2,260,211 - -0.8 2,260,211 - +0.3 2,260,211 - -1.3 2,260,211 - -0.9 2,260,211 - -0.3 2,260,211 - +1.3 2,260,229 + +2.0 2,260,229 + +1.1 2,260,230 - +0.3 2,260,230 - +1.3 2,260,230 - -0.4 2,260,230 - +0.8 2,260,263 + -0.7 2,260,263 + +0.1 2,260,263 + +0.6 2,260,263 + +2.3 2,260,263 + -0.3 2,260,264 - +0.1 2,260,264 - -0.7 2,260,280 + -1.9 2,260,281 - +0.6 2,260,288 + +0.3 2,260,288 + +0.6 2,260,288 + +1.0 2,260,288 + +1.1 2,260,288 + -1.0 2,260,289 - -0.7 2,260,289 - -1.0 2,260,289 - +0.7 2,260,289 - +0.6 2,260,291 + +2.6 2,260,350 + -0.5 2,260,350 + +0.3 2,260,350 + +1.1 2,260,350 + -0.4 2,260,350 + -0.3 2,260,350 + -0.0 2,260,351 - +1.3 2,260,351 - -0.7 2,260,351 - -1.0 2,260,351 - -0.7 2,260,354 + +0.3 2,260,354 + -0.0 2,260,354 + +0.8 2,260,354 + -0.7 2,260,355 - -0.3 2,260,355 - +0.8 2,260,364 + +0.3 2,260,373 + +0.5 2,260,373 + +0.3 2,260,373 + +1.3 2,260,373 + -0.2 2,260,373 + -0.7 2,260,373 + -0.7 2,260,373 + -0.3 2,260,374 - -0.7 2,260,374 - +1.6 2,260,374 - +1.7 2,260,374 - +1.5 2,260,374 - +0.3 2,260,374 - -0.5 2,260,432 - +1.3 2,260,466 + -0.4 2,260,466 + +1.3 2,260,466 + +0.9 2,260,466 + +0.7 2,260,466 + +0.3 2,260,466 + -0.5 2,260,466 + -1.0 2,260,466 + -1.3 2,260,466 + +0.1 2,260,466 + -0.4 2,260,466 + +0.7 2,260,466 + -1.1 2,260,466 + -1.3 2,260,466 + -1.0 2,260,466 + +0.9 2,260,466 + +0.3 2,260,466 + -1.7 2,260,467 - +2.9 2,260,467 - -0.4 2,260,467 - +0.3 2,260,467 - +1.3 2,260,467 - -0.1 2,260,467 - -0.3 2,260,467 - +1.3 2,260,467 - +0.9 2,260,475 + +0.7 2,260,762 + Pf1N1B4_1977 0.18 +0.3 2,260,762 + Pf1N1B4_1977 0.18 +0.3 2,260,832 + Pf1N1B4_1977 0.21 -1.3 2,260,864 + Pf1N1B4_1977 0.23 +0.3 2,260,864 + Pf1N1B4_1977 0.23 +0.1 2,260,864 + Pf1N1B4_1977 0.23 -0.0 2,260,865 - Pf1N1B4_1977 0.23 -0.0 2,260,865 - Pf1N1B4_1977 0.23 +1.2 2,261,084 + Pf1N1B4_1977 0.34 +1.6 2,261,084 + Pf1N1B4_1977 0.34 -0.3 2,261,084 + Pf1N1B4_1977 0.34 +0.9 2,261,085 - Pf1N1B4_1977 0.34 +1.9 2,261,085 - Pf1N1B4_1977 0.34 -1.7 2,261,085 - Pf1N1B4_1977 0.34 +2.3 2,261,085 - Pf1N1B4_1977 0.34 -1.0
Or see this region's nucleotide sequence