Experiment: Temperature shift 30_to_10; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03525 and PFLU_RS03530 are separated by 263 nucleotides PFLU_RS03530 and PFLU_RS31395 overlap by 4 nucleotides PFLU_RS31395 and PFLU_RS03535 overlap by 14 nucleotides PFLU_RS03535 and PFLU_RS03540 overlap by 4 nucleotides PFLU_RS03540 and PFLU_RS03545 overlap by 4 nucleotides
PFLU_RS03525: PFLU_RS03525 - RNA polymerase sigma factor, at 816,984 to 817,535
_RS03525
PFLU_RS03530: PFLU_RS03530 - SepL/TyeA/HrpJ family type III secretion system gatekeeper, at 817,799 to 818,353
_RS03530
PFLU_RS31395: PFLU_RS31395 - EscN/YscN/HrcN family type III secretion system ATPase, at 818,350 to 818,514
_RS31395
PFLU_RS03535: PFLU_RS03535 - YscO family type III secretion system apparatus protein, at 818,501 to 818,929
_RS03535
PFLU_RS03540: PFLU_RS03540 - type III secretion protein, at 818,926 to 819,384
_RS03540
PFLU_RS03545: PFLU_RS03545 - type III secretion protein, at 819,381 to 820,394
_RS03545
Position (kb) 818
819 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 817.512 kb on + strand at 817.529 kb on + strand at 817.553 kb on - strand at 817.553 kb on - strand at 817.682 kb on + strand at 817.682 kb on + strand at 817.683 kb on - strand at 817.683 kb on - strand at 817.733 kb on + strand at 817.733 kb on + strand at 817.733 kb on + strand at 817.733 kb on + strand at 817.734 kb on - strand at 817.734 kb on - strand at 817.933 kb on + strand, within PFLU_RS03530 at 817.933 kb on + strand, within PFLU_RS03530 at 817.933 kb on + strand, within PFLU_RS03530 at 817.933 kb on + strand, within PFLU_RS03530 at 817.933 kb on + strand, within PFLU_RS03530 at 817.934 kb on - strand, within PFLU_RS03530 at 817.934 kb on - strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 817.999 kb on + strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.000 kb on - strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.003 kb on + strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.004 kb on - strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.147 kb on + strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.148 kb on - strand, within PFLU_RS03530 at 818.231 kb on + strand, within PFLU_RS03530 at 818.232 kb on - strand, within PFLU_RS03530 at 818.232 kb on - strand, within PFLU_RS03530 at 818.314 kb on - strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.317 kb on + strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.318 kb on - strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.324 kb on + strand at 818.325 kb on - strand at 818.325 kb on - strand at 818.325 kb on - strand at 818.327 kb on + strand at 818.327 kb on + strand at 818.327 kb on + strand at 818.327 kb on + strand at 818.327 kb on + strand at 818.327 kb on + strand at 818.328 kb on - strand at 818.328 kb on - strand at 818.328 kb on - strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.500 kb on + strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.501 kb on - strand at 818.542 kb on - strand at 818.542 kb on - strand at 818.596 kb on + strand, within PFLU_RS03535 at 818.597 kb on - strand, within PFLU_RS03535 at 818.715 kb on + strand, within PFLU_RS03535 at 818.715 kb on + strand, within PFLU_RS03535 at 818.715 kb on + strand, within PFLU_RS03535 at 818.716 kb on - strand, within PFLU_RS03535 at 818.716 kb on - strand, within PFLU_RS03535 at 818.716 kb on - strand, within PFLU_RS03535 at 818.898 kb on + strand at 818.907 kb on + strand at 818.907 kb on + strand at 818.907 kb on + strand at 818.907 kb on + strand at 818.907 kb on + strand at 818.908 kb on - strand at 818.944 kb on - strand at 819.193 kb on + strand, within PFLU_RS03540 at 819.193 kb on + strand, within PFLU_RS03540 at 819.193 kb on + strand, within PFLU_RS03540 at 819.193 kb on + strand, within PFLU_RS03540 at 819.193 kb on + strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.194 kb on - strand, within PFLU_RS03540 at 819.385 kb on + strand at 819.385 kb on + strand at 819.385 kb on + strand at 819.385 kb on + strand at 819.386 kb on - strand at 819.386 kb on - strand at 819.386 kb on - strand at 819.708 kb on + strand, within PFLU_RS03545 at 819.708 kb on + strand, within PFLU_RS03545 at 819.708 kb on + strand, within PFLU_RS03545 at 819.708 kb on + strand, within PFLU_RS03545 at 819.709 kb on - strand, within PFLU_RS03545 at 819.709 kb on - strand, within PFLU_RS03545 at 819.709 kb on - strand, within PFLU_RS03545 at 819.709 kb on - strand, within PFLU_RS03545 at 819.709 kb on - strand, within PFLU_RS03545 at 819.792 kb on + strand, within PFLU_RS03545 at 819.792 kb on + strand, within PFLU_RS03545 at 819.792 kb on + strand, within PFLU_RS03545 at 819.793 kb on - strand, within PFLU_RS03545 at 819.793 kb on - strand, within PFLU_RS03545 at 819.793 kb on - strand, within PFLU_RS03545 at 819.793 kb on - strand, within PFLU_RS03545
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_10; with MOPS remove 817,512 + +0.6 817,529 + -0.2 817,553 - -0.9 817,553 - -0.5 817,682 + -1.9 817,682 + +0.2 817,683 - +0.4 817,683 - +0.8 817,733 + -1.4 817,733 + +0.3 817,733 + -0.2 817,733 + -0.6 817,734 - +0.5 817,734 - -0.2 817,933 + PFLU_RS03530 0.24 -0.7 817,933 + PFLU_RS03530 0.24 -1.4 817,933 + PFLU_RS03530 0.24 -0.2 817,933 + PFLU_RS03530 0.24 +0.4 817,933 + PFLU_RS03530 0.24 -0.4 817,934 - PFLU_RS03530 0.24 -0.7 817,934 - PFLU_RS03530 0.24 +0.8 817,999 + PFLU_RS03530 0.36 +0.9 817,999 + PFLU_RS03530 0.36 +1.1 817,999 + PFLU_RS03530 0.36 -0.3 817,999 + PFLU_RS03530 0.36 -0.4 817,999 + PFLU_RS03530 0.36 -0.3 817,999 + PFLU_RS03530 0.36 +0.5 817,999 + PFLU_RS03530 0.36 -0.3 818,000 - PFLU_RS03530 0.36 -0.2 818,000 - PFLU_RS03530 0.36 +1.6 818,000 - PFLU_RS03530 0.36 -1.7 818,000 - PFLU_RS03530 0.36 -0.4 818,000 - PFLU_RS03530 0.36 -0.7 818,000 - PFLU_RS03530 0.36 -0.4 818,000 - PFLU_RS03530 0.36 -0.6 818,000 - PFLU_RS03530 0.36 -0.4 818,000 - PFLU_RS03530 0.36 +0.4 818,000 - PFLU_RS03530 0.36 -0.8 818,003 + PFLU_RS03530 0.37 -0.2 818,003 + PFLU_RS03530 0.37 -1.5 818,003 + PFLU_RS03530 0.37 +0.3 818,003 + PFLU_RS03530 0.37 +0.9 818,003 + PFLU_RS03530 0.37 +1.1 818,003 + PFLU_RS03530 0.37 +4.2 818,003 + PFLU_RS03530 0.37 +1.2 818,003 + PFLU_RS03530 0.37 -0.6 818,003 + PFLU_RS03530 0.37 +0.6 818,003 + PFLU_RS03530 0.37 +0.6 818,003 + PFLU_RS03530 0.37 -0.2 818,003 + PFLU_RS03530 0.37 +1.2 818,003 + PFLU_RS03530 0.37 -0.4 818,003 + PFLU_RS03530 0.37 -1.4 818,003 + PFLU_RS03530 0.37 +1.2 818,003 + PFLU_RS03530 0.37 +0.1 818,003 + PFLU_RS03530 0.37 -1.2 818,003 + PFLU_RS03530 0.37 -0.2 818,003 + PFLU_RS03530 0.37 +0.3 818,003 + PFLU_RS03530 0.37 -0.9 818,003 + PFLU_RS03530 0.37 +0.4 818,004 - PFLU_RS03530 0.37 -0.8 818,004 - PFLU_RS03530 0.37 +0.3 818,004 - PFLU_RS03530 0.37 +1.4 818,004 - PFLU_RS03530 0.37 -0.6 818,004 - PFLU_RS03530 0.37 +0.2 818,004 - PFLU_RS03530 0.37 -0.1 818,004 - PFLU_RS03530 0.37 -1.4 818,004 - PFLU_RS03530 0.37 +0.2 818,004 - PFLU_RS03530 0.37 +0.2 818,004 - PFLU_RS03530 0.37 -0.8 818,004 - PFLU_RS03530 0.37 +0.2 818,004 - PFLU_RS03530 0.37 +0.3 818,004 - PFLU_RS03530 0.37 +0.3 818,004 - PFLU_RS03530 0.37 +0.5 818,004 - PFLU_RS03530 0.37 -0.2 818,004 - PFLU_RS03530 0.37 -0.2 818,004 - PFLU_RS03530 0.37 -0.2 818,004 - PFLU_RS03530 0.37 +0.7 818,004 - PFLU_RS03530 0.37 -1.3 818,004 - PFLU_RS03530 0.37 -0.9 818,004 - PFLU_RS03530 0.37 +0.5 818,004 - PFLU_RS03530 0.37 +2.0 818,004 - PFLU_RS03530 0.37 +2.2 818,004 - PFLU_RS03530 0.37 +0.1 818,004 - PFLU_RS03530 0.37 -1.8 818,004 - PFLU_RS03530 0.37 +0.3 818,004 - PFLU_RS03530 0.37 -0.8 818,004 - PFLU_RS03530 0.37 +1.4 818,004 - PFLU_RS03530 0.37 -0.6 818,004 - PFLU_RS03530 0.37 +0.8 818,004 - PFLU_RS03530 0.37 +0.6 818,147 + PFLU_RS03530 0.63 +0.9 818,147 + PFLU_RS03530 0.63 -0.9 818,147 + PFLU_RS03530 0.63 -0.2 818,147 + PFLU_RS03530 0.63 -0.2 818,147 + PFLU_RS03530 0.63 +0.5 818,147 + PFLU_RS03530 0.63 +0.4 818,147 + PFLU_RS03530 0.63 +0.3 818,147 + PFLU_RS03530 0.63 -1.2 818,148 - PFLU_RS03530 0.63 -0.5 818,148 - PFLU_RS03530 0.63 +1.2 818,148 - PFLU_RS03530 0.63 -0.4 818,148 - PFLU_RS03530 0.63 -0.1 818,148 - PFLU_RS03530 0.63 -0.3 818,148 - PFLU_RS03530 0.63 -2.4 818,148 - PFLU_RS03530 0.63 +0.9 818,148 - PFLU_RS03530 0.63 +1.0 818,231 + PFLU_RS03530 0.78 +0.4 818,232 - PFLU_RS03530 0.78 -0.4 818,232 - PFLU_RS03530 0.78 +0.6 818,314 - -0.3 818,317 + -1.2 818,317 + +0.2 818,317 + -0.6 818,317 + -0.5 818,317 + -1.9 818,317 + -0.7 818,317 + -0.1 818,317 + +0.8 818,317 + +1.3 818,317 + +2.2 818,318 - -0.5 818,318 - -0.2 818,318 - -0.7 818,318 - -0.9 818,318 - -0.8 818,318 - +0.5 818,318 - +0.8 818,324 + +0.1 818,324 + -1.9 818,324 + +2.4 818,324 + +1.1 818,324 + -0.1 818,324 + +0.9 818,324 + +0.2 818,324 + +0.2 818,324 + +0.5 818,325 - +0.1 818,325 - -1.5 818,325 - +0.2 818,327 + -0.4 818,327 + -0.9 818,327 + +0.1 818,327 + -0.4 818,327 + +0.4 818,327 + -1.9 818,328 - +0.2 818,328 - -0.0 818,328 - -0.9 818,500 + +0.9 818,500 + +0.4 818,500 + +0.2 818,500 + -1.2 818,500 + -0.5 818,500 + -0.2 818,500 + -0.1 818,500 + -0.6 818,500 + +0.4 818,500 + -0.4 818,500 + +0.1 818,500 + -1.0 818,500 + -0.6 818,500 + -1.1 818,500 + +0.6 818,500 + -0.1 818,500 + +1.1 818,500 + -0.7 818,500 + +0.6 818,500 + +0.6 818,500 + +0.4 818,500 + +0.1 818,500 + -0.2 818,500 + +0.9 818,500 + +1.2 818,500 + +0.1 818,500 + -0.7 818,500 + +0.6 818,500 + +1.1 818,500 + -0.1 818,501 - -1.4 818,501 - +0.2 818,501 - -0.5 818,501 - -0.4 818,501 - +0.2 818,501 - +0.5 818,501 - -0.9 818,501 - -1.4 818,501 - -0.6 818,501 - +1.1 818,501 - -0.1 818,501 - -0.4 818,501 - +0.8 818,501 - -0.2 818,501 - +0.5 818,501 - -0.6 818,501 - -1.0 818,501 - +0.3 818,501 - -0.1 818,501 - -1.7 818,501 - +0.2 818,501 - -0.1 818,501 - -1.1 818,501 - +0.3 818,501 - +1.0 818,501 - -0.7 818,501 - -1.5 818,501 - +0.4 818,501 - -0.5 818,501 - -0.4 818,501 - -0.9 818,501 - -0.4 818,501 - -0.1 818,501 - +0.3 818,501 - -0.5 818,501 - -2.5 818,501 - -0.7 818,501 - -0.8 818,501 - -1.1 818,542 - -0.1 818,542 - -0.5 818,596 + PFLU_RS03535 0.22 -0.2 818,597 - PFLU_RS03535 0.22 +0.6 818,715 + PFLU_RS03535 0.50 +0.1 818,715 + PFLU_RS03535 0.50 -1.6 818,715 + PFLU_RS03535 0.50 -0.4 818,716 - PFLU_RS03535 0.50 +0.4 818,716 - PFLU_RS03535 0.50 -1.5 818,716 - PFLU_RS03535 0.50 -1.3 818,898 + +0.1 818,907 + -0.7 818,907 + -0.7 818,907 + +0.1 818,907 + +0.6 818,907 + +1.3 818,908 - -0.2 818,944 - -0.5 819,193 + PFLU_RS03540 0.58 -1.4 819,193 + PFLU_RS03540 0.58 -0.7 819,193 + PFLU_RS03540 0.58 +0.4 819,193 + PFLU_RS03540 0.58 +0.1 819,193 + PFLU_RS03540 0.58 +0.3 819,194 - PFLU_RS03540 0.58 -0.9 819,194 - PFLU_RS03540 0.58 -0.5 819,194 - PFLU_RS03540 0.58 +0.3 819,194 - PFLU_RS03540 0.58 -0.8 819,194 - PFLU_RS03540 0.58 -0.2 819,194 - PFLU_RS03540 0.58 -1.0 819,194 - PFLU_RS03540 0.58 +1.4 819,385 + -0.6 819,385 + -1.0 819,385 + -0.7 819,385 + -0.3 819,386 - -0.9 819,386 - -1.0 819,386 - +1.6 819,708 + PFLU_RS03545 0.32 +0.8 819,708 + PFLU_RS03545 0.32 +0.1 819,708 + PFLU_RS03545 0.32 +0.4 819,708 + PFLU_RS03545 0.32 +1.3 819,709 - PFLU_RS03545 0.32 +0.1 819,709 - PFLU_RS03545 0.32 +0.6 819,709 - PFLU_RS03545 0.32 -0.2 819,709 - PFLU_RS03545 0.32 +0.3 819,709 - PFLU_RS03545 0.32 -0.7 819,792 + PFLU_RS03545 0.41 +0.5 819,792 + PFLU_RS03545 0.41 -0.2 819,792 + PFLU_RS03545 0.41 +0.8 819,793 - PFLU_RS03545 0.41 -0.5 819,793 - PFLU_RS03545 0.41 +0.3 819,793 - PFLU_RS03545 0.41 +0.1 819,793 - PFLU_RS03545 0.41 +0.4
Or see this region's nucleotide sequence