Experiment: Temperature shift 30_to_10; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02350 and PFLU_RS02355 are separated by 0 nucleotides PFLU_RS02355 and PFLU_RS02360 overlap by 4 nucleotides
PFLU_RS02350: PFLU_RS02350 - carbamoyltransferase, at 537,073 to 538,830
_RS02350
PFLU_RS02355: PFLU_RS02355 - GNAT family N-acetyltransferase, at 538,831 to 539,727
_RS02355
PFLU_RS02360: PFLU_RS02360 - PIG-L family deacetylase, at 539,724 to 541,124
_RS02360
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 537.844 kb on + strand, within PFLU_RS02350 at 537.844 kb on + strand, within PFLU_RS02350 at 537.844 kb on + strand, within PFLU_RS02350 at 537.844 kb on + strand, within PFLU_RS02350 at 537.844 kb on + strand, within PFLU_RS02350 at 537.844 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.910 kb on + strand, within PFLU_RS02350 at 537.919 kb on + strand, within PFLU_RS02350 at 537.919 kb on + strand, within PFLU_RS02350 at 537.976 kb on + strand, within PFLU_RS02350 at 537.976 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.138 kb on + strand, within PFLU_RS02350 at 538.186 kb on + strand, within PFLU_RS02350 at 538.186 kb on + strand, within PFLU_RS02350 at 538.186 kb on + strand, within PFLU_RS02350 at 538.201 kb on + strand, within PFLU_RS02350 at 538.201 kb on + strand, within PFLU_RS02350 at 538.278 kb on + strand, within PFLU_RS02350 at 538.278 kb on + strand, within PFLU_RS02350 at 538.615 kb on + strand, within PFLU_RS02350 at 538.615 kb on + strand, within PFLU_RS02350 at 538.630 kb on + strand, within PFLU_RS02350 at 538.630 kb on + strand, within PFLU_RS02350 at 538.630 kb on + strand, within PFLU_RS02350 at 538.630 kb on + strand, within PFLU_RS02350 at 538.655 kb on + strand at 538.655 kb on + strand at 538.655 kb on + strand at 538.765 kb on + strand at 538.765 kb on + strand at 538.765 kb on + strand at 538.766 kb on - strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.813 kb on + strand at 538.814 kb on - strand at 538.814 kb on - strand at 538.814 kb on - strand at 538.829 kb on - strand at 538.829 kb on - strand at 538.829 kb on - strand at 538.891 kb on + strand at 538.985 kb on - strand, within PFLU_RS02355 at 539.167 kb on + strand, within PFLU_RS02355 at 539.168 kb on - strand, within PFLU_RS02355 at 539.270 kb on - strand, within PFLU_RS02355 at 539.344 kb on - strand, within PFLU_RS02355 at 539.362 kb on + strand, within PFLU_RS02355 at 539.362 kb on + strand, within PFLU_RS02355 at 539.363 kb on - strand, within PFLU_RS02355 at 539.363 kb on - strand, within PFLU_RS02355 at 539.503 kb on + strand, within PFLU_RS02355 at 539.504 kb on - strand, within PFLU_RS02355 at 539.504 kb on - strand, within PFLU_RS02355 at 539.539 kb on + strand, within PFLU_RS02355 at 539.539 kb on + strand, within PFLU_RS02355 at 539.539 kb on + strand, within PFLU_RS02355 at 539.539 kb on + strand, within PFLU_RS02355 at 539.539 kb on + strand, within PFLU_RS02355 at 539.540 kb on - strand, within PFLU_RS02355 at 539.540 kb on - strand, within PFLU_RS02355 at 539.540 kb on - strand, within PFLU_RS02355 at 539.540 kb on - strand, within PFLU_RS02355 at 539.540 kb on - strand, within PFLU_RS02355 at 539.591 kb on + strand, within PFLU_RS02355 at 539.591 kb on + strand, within PFLU_RS02355 at 539.591 kb on + strand, within PFLU_RS02355 at 539.591 kb on + strand, within PFLU_RS02355 at 539.591 kb on + strand, within PFLU_RS02355 at 539.683 kb on + strand at 539.684 kb on - strand at 539.684 kb on - strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.724 kb on - strand at 539.724 kb on - strand at 539.724 kb on - strand at 539.724 kb on - strand at 539.724 kb on - strand at 539.724 kb on - strand at 539.766 kb on - strand at 539.898 kb on + strand, within PFLU_RS02360 at 539.916 kb on + strand, within PFLU_RS02360 at 539.916 kb on + strand, within PFLU_RS02360 at 539.916 kb on + strand, within PFLU_RS02360 at 539.916 kb on + strand, within PFLU_RS02360 at 539.917 kb on - strand, within PFLU_RS02360 at 539.917 kb on - strand, within PFLU_RS02360 at 539.917 kb on - strand, within PFLU_RS02360 at 539.923 kb on - strand, within PFLU_RS02360 at 540.031 kb on + strand, within PFLU_RS02360 at 540.031 kb on + strand, within PFLU_RS02360 at 540.031 kb on + strand, within PFLU_RS02360 at 540.031 kb on + strand, within PFLU_RS02360 at 540.032 kb on - strand, within PFLU_RS02360 at 540.032 kb on - strand, within PFLU_RS02360 at 540.032 kb on - strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.049 kb on + strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.050 kb on - strand, within PFLU_RS02360 at 540.129 kb on + strand, within PFLU_RS02360 at 540.129 kb on + strand, within PFLU_RS02360 at 540.129 kb on + strand, within PFLU_RS02360 at 540.130 kb on - strand, within PFLU_RS02360 at 540.130 kb on - strand, within PFLU_RS02360 at 540.313 kb on - strand, within PFLU_RS02360 at 540.313 kb on - strand, within PFLU_RS02360 at 540.313 kb on - strand, within PFLU_RS02360 at 540.313 kb on - strand, within PFLU_RS02360 at 540.376 kb on - strand, within PFLU_RS02360 at 540.376 kb on - strand, within PFLU_RS02360
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_10; with MOPS remove 537,844 + PFLU_RS02350 0.44 +0.5 537,844 + PFLU_RS02350 0.44 +1.8 537,844 + PFLU_RS02350 0.44 -1.5 537,844 + PFLU_RS02350 0.44 -1.0 537,844 + PFLU_RS02350 0.44 -1.0 537,844 + PFLU_RS02350 0.44 +0.6 537,910 + PFLU_RS02350 0.48 -1.2 537,910 + PFLU_RS02350 0.48 -1.7 537,910 + PFLU_RS02350 0.48 -2.5 537,910 + PFLU_RS02350 0.48 -0.4 537,910 + PFLU_RS02350 0.48 -1.1 537,910 + PFLU_RS02350 0.48 -0.2 537,910 + PFLU_RS02350 0.48 -0.5 537,910 + PFLU_RS02350 0.48 +0.8 537,910 + PFLU_RS02350 0.48 -0.2 537,910 + PFLU_RS02350 0.48 -1.9 537,910 + PFLU_RS02350 0.48 -0.4 537,919 + PFLU_RS02350 0.48 +1.4 537,919 + PFLU_RS02350 0.48 -2.5 537,976 + PFLU_RS02350 0.51 -0.2 537,976 + PFLU_RS02350 0.51 -1.3 538,138 + PFLU_RS02350 0.61 -0.8 538,138 + PFLU_RS02350 0.61 +0.1 538,138 + PFLU_RS02350 0.61 +0.8 538,138 + PFLU_RS02350 0.61 +0.9 538,138 + PFLU_RS02350 0.61 -0.1 538,138 + PFLU_RS02350 0.61 -0.2 538,186 + PFLU_RS02350 0.63 +0.2 538,186 + PFLU_RS02350 0.63 -0.2 538,186 + PFLU_RS02350 0.63 -1.8 538,201 + PFLU_RS02350 0.64 -1.2 538,201 + PFLU_RS02350 0.64 -1.8 538,278 + PFLU_RS02350 0.69 -1.5 538,278 + PFLU_RS02350 0.69 +0.9 538,615 + PFLU_RS02350 0.88 +1.1 538,615 + PFLU_RS02350 0.88 +0.7 538,630 + PFLU_RS02350 0.89 -1.0 538,630 + PFLU_RS02350 0.89 -1.7 538,630 + PFLU_RS02350 0.89 -1.9 538,630 + PFLU_RS02350 0.89 +0.3 538,655 + -0.1 538,655 + +1.8 538,655 + +0.9 538,765 + -1.4 538,765 + +0.6 538,765 + -1.0 538,766 - -0.2 538,813 + +0.3 538,813 + +1.2 538,813 + -0.8 538,813 + +1.9 538,813 + +1.2 538,813 + -0.9 538,813 + +0.1 538,813 + +0.2 538,814 - +0.2 538,814 - +0.8 538,814 - -1.0 538,829 - -0.2 538,829 - -1.3 538,829 - -1.2 538,891 + +2.6 538,985 - PFLU_RS02355 0.17 -1.4 539,167 + PFLU_RS02355 0.37 +1.8 539,168 - PFLU_RS02355 0.38 +0.5 539,270 - PFLU_RS02355 0.49 +0.8 539,344 - PFLU_RS02355 0.57 -1.2 539,362 + PFLU_RS02355 0.59 +0.2 539,362 + PFLU_RS02355 0.59 +1.8 539,363 - PFLU_RS02355 0.59 +0.8 539,363 - PFLU_RS02355 0.59 -0.3 539,503 + PFLU_RS02355 0.75 +0.2 539,504 - PFLU_RS02355 0.75 +0.4 539,504 - PFLU_RS02355 0.75 -0.7 539,539 + PFLU_RS02355 0.79 +1.8 539,539 + PFLU_RS02355 0.79 +3.6 539,539 + PFLU_RS02355 0.79 -3.1 539,539 + PFLU_RS02355 0.79 -2.1 539,539 + PFLU_RS02355 0.79 +0.2 539,540 - PFLU_RS02355 0.79 -1.5 539,540 - PFLU_RS02355 0.79 -2.4 539,540 - PFLU_RS02355 0.79 -0.5 539,540 - PFLU_RS02355 0.79 -1.3 539,540 - PFLU_RS02355 0.79 +0.2 539,591 + PFLU_RS02355 0.85 -0.4 539,591 + PFLU_RS02355 0.85 -0.8 539,591 + PFLU_RS02355 0.85 -1.9 539,591 + PFLU_RS02355 0.85 -1.0 539,591 + PFLU_RS02355 0.85 -0.3 539,683 + +1.8 539,684 - +0.8 539,684 - +0.2 539,723 + +0.9 539,723 + -0.5 539,723 + +0.4 539,723 + +1.5 539,723 + -2.5 539,723 + -1.7 539,723 + +0.2 539,723 + -0.1 539,723 + +0.3 539,724 - +0.2 539,724 - +0.2 539,724 - +0.2 539,724 - -0.9 539,724 - +1.8 539,724 - -0.5 539,766 - -0.5 539,898 + PFLU_RS02360 0.12 +2.4 539,916 + PFLU_RS02360 0.14 -2.5 539,916 + PFLU_RS02360 0.14 +0.8 539,916 + PFLU_RS02360 0.14 +0.1 539,916 + PFLU_RS02360 0.14 +2.2 539,917 - PFLU_RS02360 0.14 +0.8 539,917 - PFLU_RS02360 0.14 -2.5 539,917 - PFLU_RS02360 0.14 -0.4 539,923 - PFLU_RS02360 0.14 +0.0 540,031 + PFLU_RS02360 0.22 +0.3 540,031 + PFLU_RS02360 0.22 +0.1 540,031 + PFLU_RS02360 0.22 -0.6 540,031 + PFLU_RS02360 0.22 +1.8 540,032 - PFLU_RS02360 0.22 -0.7 540,032 - PFLU_RS02360 0.22 +1.0 540,032 - PFLU_RS02360 0.22 -1.0 540,049 + PFLU_RS02360 0.23 -2.6 540,049 + PFLU_RS02360 0.23 -0.8 540,049 + PFLU_RS02360 0.23 -0.2 540,049 + PFLU_RS02360 0.23 -1.4 540,049 + PFLU_RS02360 0.23 +0.8 540,049 + PFLU_RS02360 0.23 +0.3 540,049 + PFLU_RS02360 0.23 -0.8 540,050 - PFLU_RS02360 0.23 -1.3 540,050 - PFLU_RS02360 0.23 -1.2 540,050 - PFLU_RS02360 0.23 -0.8 540,050 - PFLU_RS02360 0.23 -2.0 540,050 - PFLU_RS02360 0.23 -1.1 540,050 - PFLU_RS02360 0.23 -1.6 540,050 - PFLU_RS02360 0.23 +0.5 540,050 - PFLU_RS02360 0.23 -0.4 540,050 - PFLU_RS02360 0.23 -0.2 540,050 - PFLU_RS02360 0.23 -1.3 540,129 + PFLU_RS02360 0.29 +1.2 540,129 + PFLU_RS02360 0.29 +0.2 540,129 + PFLU_RS02360 0.29 -0.0 540,130 - PFLU_RS02360 0.29 -0.2 540,130 - PFLU_RS02360 0.29 +0.5 540,313 - PFLU_RS02360 0.42 +0.2 540,313 - PFLU_RS02360 0.42 -2.4 540,313 - PFLU_RS02360 0.42 -0.8 540,313 - PFLU_RS02360 0.42 -1.8 540,376 - PFLU_RS02360 0.47 -1.7 540,376 - PFLU_RS02360 0.47 +0.7
Or see this region's nucleotide sequence