Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05543 and H281DRAFT_05544 are separated by 250 nucleotides H281DRAFT_05544 and H281DRAFT_05545 are separated by 7 nucleotides H281DRAFT_05545 and H281DRAFT_05546 are separated by 312 nucleotides
H281DRAFT_05543: H281DRAFT_05543 - porin, GBP family, at 130,495 to 131,649
_05543
H281DRAFT_05544: H281DRAFT_05544 - NitT/TauT family transport system permease protein, at 131,900 to 132,775
_05544
H281DRAFT_05545: H281DRAFT_05545 - NitT/TauT family transport system ATP-binding protein, at 132,783 to 133,379
_05545
H281DRAFT_05546: H281DRAFT_05546 - NitT/TauT family transport system substrate-binding protein, at 133,692 to 134,699
_05546
Position (kb) 131
132
133 Strain fitness (log2 ratio) -1
0
1 at 130.965 kb on - strand at 131.097 kb on + strand, within H281DRAFT_05543 at 131.156 kb on + strand, within H281DRAFT_05543 at 131.157 kb on - strand, within H281DRAFT_05543 at 131.327 kb on + strand, within H281DRAFT_05543 at 131.449 kb on + strand, within H281DRAFT_05543 at 131.491 kb on - strand, within H281DRAFT_05543 at 131.988 kb on - strand, within H281DRAFT_05544 at 132.550 kb on - strand, within H281DRAFT_05544 at 132.776 kb on - strand at 132.776 kb on - strand at 132.776 kb on - strand at 132.922 kb on + strand, within H281DRAFT_05545 at 133.464 kb on + strand at 133.464 kb on + strand at 133.465 kb on - strand at 133.554 kb on + strand at 133.607 kb on + strand at 133.607 kb on + strand at 133.607 kb on + strand at 133.625 kb on + strand at 133.630 kb on + strand at 133.633 kb on + strand at 133.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 130,965 - +1.3 131,097 + H281DRAFT_05543 0.52 +0.7 131,156 + H281DRAFT_05543 0.57 +0.6 131,157 - H281DRAFT_05543 0.57 -0.9 131,327 + H281DRAFT_05543 0.72 -0.6 131,449 + H281DRAFT_05543 0.83 -1.5 131,491 - H281DRAFT_05543 0.86 +0.2 131,988 - H281DRAFT_05544 0.10 +1.1 132,550 - H281DRAFT_05544 0.74 +0.4 132,776 - -1.5 132,776 - +0.8 132,776 - +0.6 132,922 + H281DRAFT_05545 0.23 +0.7 133,464 + -0.7 133,464 + +0.6 133,465 - -1.1 133,554 + +0.1 133,607 + -0.1 133,607 + +0.4 133,607 + -0.4 133,625 + -0.7 133,630 + +0.1 133,633 + +0.8 133,673 + -0.2
Or see this region's nucleotide sequence