Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05328 and H281DRAFT_05329 are separated by 124 nucleotides H281DRAFT_05329 and H281DRAFT_05330 are separated by 54 nucleotides H281DRAFT_05330 and H281DRAFT_05331 are separated by 247 nucleotides H281DRAFT_05331 and H281DRAFT_05332 overlap by 26 nucleotides
H281DRAFT_05328: H281DRAFT_05328 - 4-oxalocrotonate tautomerase, at 79,583 to 79,774
_05328
H281DRAFT_05329: H281DRAFT_05329 - Ribulose-5-phosphate 4-epimerase/Fuculose-1-phosphate aldolase, at 79,899 to 80,675
_05329
H281DRAFT_05330: H281DRAFT_05330 - urea transporter, at 80,730 to 81,593
_05330
H281DRAFT_05331: H281DRAFT_05331 - hypothetical protein, at 81,841 to 82,395
_05331
H281DRAFT_05332: H281DRAFT_05332 - Pimeloyl-ACP methyl ester carboxylesterase, at 82,370 to 83,314
_05332
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 79.803 kb on + strand at 79.867 kb on + strand at 79.868 kb on - strand at 79.868 kb on - strand at 79.984 kb on + strand, within H281DRAFT_05329 at 80.101 kb on + strand, within H281DRAFT_05329 at 80.102 kb on - strand, within H281DRAFT_05329 at 80.102 kb on - strand, within H281DRAFT_05329 at 80.249 kb on - strand, within H281DRAFT_05329 at 80.424 kb on - strand, within H281DRAFT_05329 at 80.540 kb on + strand, within H281DRAFT_05329 at 80.543 kb on - strand, within H281DRAFT_05329 at 80.675 kb on + strand at 80.675 kb on + strand at 80.676 kb on - strand at 81.128 kb on + strand, within H281DRAFT_05330 at 81.218 kb on - strand, within H281DRAFT_05330 at 81.218 kb on - strand, within H281DRAFT_05330 at 81.367 kb on + strand at 81.793 kb on - strand at 81.827 kb on + strand at 81.851 kb on + strand at 81.975 kb on + strand, within H281DRAFT_05331 at 82.150 kb on - strand, within H281DRAFT_05331 at 82.152 kb on + strand, within H281DRAFT_05331 at 82.202 kb on + strand, within H281DRAFT_05331 at 82.451 kb on + strand at 82.521 kb on + strand, within H281DRAFT_05332
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 79,803 + +2.2 79,867 + +0.1 79,868 - +0.8 79,868 - -0.7 79,984 + H281DRAFT_05329 0.11 +0.2 80,101 + H281DRAFT_05329 0.26 -2.4 80,102 - H281DRAFT_05329 0.26 +0.2 80,102 - H281DRAFT_05329 0.26 +0.7 80,249 - H281DRAFT_05329 0.45 +0.3 80,424 - H281DRAFT_05329 0.68 -0.0 80,540 + H281DRAFT_05329 0.82 +0.7 80,543 - H281DRAFT_05329 0.83 -0.3 80,675 + -0.4 80,675 + +0.1 80,676 - +0.4 81,128 + H281DRAFT_05330 0.46 +1.2 81,218 - H281DRAFT_05330 0.56 +0.8 81,218 - H281DRAFT_05330 0.56 -0.4 81,367 + +0.4 81,793 - +0.1 81,827 + +0.9 81,851 + -0.3 81,975 + H281DRAFT_05331 0.24 +0.4 82,150 - H281DRAFT_05331 0.56 -0.6 82,152 + H281DRAFT_05331 0.56 +0.1 82,202 + H281DRAFT_05331 0.65 +0.4 82,451 + +0.1 82,521 + H281DRAFT_05332 0.16 -0.3
Or see this region's nucleotide sequence