Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05322

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05320 and H281DRAFT_05321 are separated by 125 nucleotidesH281DRAFT_05321 and H281DRAFT_05322 overlap by 4 nucleotidesH281DRAFT_05322 and H281DRAFT_05323 are separated by 357 nucleotidesH281DRAFT_05323 and H281DRAFT_05324 overlap by 4 nucleotides H281DRAFT_05320: H281DRAFT_05320 - galactarate dehydratase, at 71,056 to 72,645 _05320 H281DRAFT_05321: H281DRAFT_05321 - uronate dehydrogenase, at 72,771 to 73,613 _05321 H281DRAFT_05322: H281DRAFT_05322 - gluconolactonase (EC 3.1.1.17), at 73,610 to 74,563 _05322 H281DRAFT_05323: H281DRAFT_05323 - hypothetical protein, at 74,921 to 75,211 _05323 H281DRAFT_05324: H281DRAFT_05324 - tripartite ATP-independent transporter solute receptor, DctP family, at 75,208 to 76,203 _05324 Position (kb) 73 74 75Strain fitness (log2 ratio) -2 -1 0 1 2 3at 72.684 kb on + strandat 72.685 kb on - strandat 72.720 kb on + strandat 72.720 kb on + strandat 72.721 kb on - strandat 72.751 kb on + strandat 72.751 kb on + strandat 72.761 kb on + strandat 73.089 kb on + strand, within H281DRAFT_05321at 73.251 kb on + strand, within H281DRAFT_05321at 73.254 kb on + strand, within H281DRAFT_05321at 73.254 kb on + strand, within H281DRAFT_05321at 73.255 kb on - strand, within H281DRAFT_05321at 73.255 kb on - strand, within H281DRAFT_05321at 73.350 kb on + strand, within H281DRAFT_05321at 73.417 kb on - strand, within H281DRAFT_05321at 73.582 kb on + strandat 73.715 kb on + strand, within H281DRAFT_05322at 74.169 kb on - strand, within H281DRAFT_05322at 74.169 kb on - strand, within H281DRAFT_05322at 74.319 kb on - strand, within H281DRAFT_05322at 74.729 kb on - strandat 74.826 kb on + strandat 74.982 kb on - strand, within H281DRAFT_05323at 74.982 kb on - strand, within H281DRAFT_05323at 74.982 kb on - strand, within H281DRAFT_05323

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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72,684 + -0.7
72,685 - -0.1
72,720 + -1.5
72,720 + +0.6
72,721 - +0.2
72,751 + -0.0
72,751 + +0.6
72,761 + +0.9
73,089 + H281DRAFT_05321 0.38 +0.9
73,251 + H281DRAFT_05321 0.57 -1.7
73,254 + H281DRAFT_05321 0.57 -2.1
73,254 + H281DRAFT_05321 0.57 +1.8
73,255 - H281DRAFT_05321 0.57 +0.9
73,255 - H281DRAFT_05321 0.57 +0.6
73,350 + H281DRAFT_05321 0.69 +0.1
73,417 - H281DRAFT_05321 0.77 -0.4
73,582 + +0.6
73,715 + H281DRAFT_05322 0.11 +0.8
74,169 - H281DRAFT_05322 0.59 -0.0
74,169 - H281DRAFT_05322 0.59 +3.1
74,319 - H281DRAFT_05322 0.74 -0.4
74,729 - -0.7
74,826 + -1.3
74,982 - H281DRAFT_05323 0.21 +0.4
74,982 - H281DRAFT_05323 0.21 -1.7
74,982 - H281DRAFT_05323 0.21 +1.7

Or see this region's nucleotide sequence