Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04848 and H281DRAFT_04849 overlap by 4 nucleotides H281DRAFT_04849 and H281DRAFT_04850 are separated by 14 nucleotides H281DRAFT_04850 and H281DRAFT_04851 are separated by 5 nucleotides
H281DRAFT_04848: H281DRAFT_04848 - hypothetical protein, at 290,597 to 291,442
_04848
H281DRAFT_04849: H281DRAFT_04849 - UDP-glucose 4-epimerase, at 291,439 to 292,395
_04849
H281DRAFT_04850: H281DRAFT_04850 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 292,410 to 293,435
_04850
H281DRAFT_04851: H281DRAFT_04851 - NDP-sugar epimerase, includes UDP-GlcNAc-inverting 4,6-dehydratase FlaA1 and capsular polysaccharide biosynthesis protein EpsC, at 293,441 to 295,324
_04851
Position (kb)
292
293
294 Strain fitness (log2 ratio)
-2
-1
0
1 at 291.933 kb on - strand, within H281DRAFT_04849 at 291.956 kb on - strand, within H281DRAFT_04849 at 292.058 kb on - strand, within H281DRAFT_04849 at 293.148 kb on - strand, within H281DRAFT_04850 at 293.209 kb on - strand, within H281DRAFT_04850 at 293.209 kb on - strand, within H281DRAFT_04850 at 293.209 kb on - strand, within H281DRAFT_04850 at 293.263 kb on - strand, within H281DRAFT_04850 at 293.431 kb on + strand at 293.431 kb on + strand at 293.431 kb on + strand at 293.526 kb on - strand at 293.636 kb on + strand, within H281DRAFT_04851 at 293.843 kb on + strand, within H281DRAFT_04851 at 293.974 kb on + strand, within H281DRAFT_04851 at 294.005 kb on + strand, within H281DRAFT_04851 at 294.066 kb on - strand, within H281DRAFT_04851 at 294.348 kb on + strand, within H281DRAFT_04851 at 294.431 kb on + strand, within H281DRAFT_04851
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 291,933 - H281DRAFT_04849 0.52 +0.2 291,956 - H281DRAFT_04849 0.54 -0.2 292,058 - H281DRAFT_04849 0.65 +0.6 293,148 - H281DRAFT_04850 0.72 +1.2 293,209 - H281DRAFT_04850 0.78 +1.2 293,209 - H281DRAFT_04850 0.78 -0.1 293,209 - H281DRAFT_04850 0.78 +0.6 293,263 - H281DRAFT_04850 0.83 +0.7 293,431 + -1.5 293,431 + -0.5 293,431 + +1.0 293,526 - -2.3 293,636 + H281DRAFT_04851 0.10 +0.4 293,843 + H281DRAFT_04851 0.21 +0.3 293,974 + H281DRAFT_04851 0.28 -2.6 294,005 + H281DRAFT_04851 0.30 -1.1 294,066 - H281DRAFT_04851 0.33 -2.5 294,348 + H281DRAFT_04851 0.48 -1.3 294,431 + H281DRAFT_04851 0.53 -2.4
Or see this region's nucleotide sequence