Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04850

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04848 and H281DRAFT_04849 overlap by 4 nucleotidesH281DRAFT_04849 and H281DRAFT_04850 are separated by 14 nucleotidesH281DRAFT_04850 and H281DRAFT_04851 are separated by 5 nucleotides H281DRAFT_04848: H281DRAFT_04848 - hypothetical protein, at 290,597 to 291,442 _04848 H281DRAFT_04849: H281DRAFT_04849 - UDP-glucose 4-epimerase, at 291,439 to 292,395 _04849 H281DRAFT_04850: H281DRAFT_04850 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 292,410 to 293,435 _04850 H281DRAFT_04851: H281DRAFT_04851 - NDP-sugar epimerase, includes UDP-GlcNAc-inverting 4,6-dehydratase FlaA1 and capsular polysaccharide biosynthesis protein EpsC, at 293,441 to 295,324 _04851 Position (kb) 292 293 294Strain fitness (log2 ratio) -2 -1 0 1at 291.933 kb on - strand, within H281DRAFT_04849at 291.956 kb on - strand, within H281DRAFT_04849at 292.058 kb on - strand, within H281DRAFT_04849at 293.148 kb on - strand, within H281DRAFT_04850at 293.209 kb on - strand, within H281DRAFT_04850at 293.209 kb on - strand, within H281DRAFT_04850at 293.209 kb on - strand, within H281DRAFT_04850at 293.263 kb on - strand, within H281DRAFT_04850at 293.431 kb on + strandat 293.431 kb on + strandat 293.431 kb on + strandat 293.526 kb on - strandat 293.636 kb on + strand, within H281DRAFT_04851at 293.843 kb on + strand, within H281DRAFT_04851at 293.974 kb on + strand, within H281DRAFT_04851at 294.005 kb on + strand, within H281DRAFT_04851at 294.066 kb on - strand, within H281DRAFT_04851at 294.348 kb on + strand, within H281DRAFT_04851at 294.431 kb on + strand, within H281DRAFT_04851

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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291,933 - H281DRAFT_04849 0.52 +0.2
291,956 - H281DRAFT_04849 0.54 -0.2
292,058 - H281DRAFT_04849 0.65 +0.6
293,148 - H281DRAFT_04850 0.72 +1.2
293,209 - H281DRAFT_04850 0.78 +1.2
293,209 - H281DRAFT_04850 0.78 -0.1
293,209 - H281DRAFT_04850 0.78 +0.6
293,263 - H281DRAFT_04850 0.83 +0.7
293,431 + -1.5
293,431 + -0.5
293,431 + +1.0
293,526 - -2.3
293,636 + H281DRAFT_04851 0.10 +0.4
293,843 + H281DRAFT_04851 0.21 +0.3
293,974 + H281DRAFT_04851 0.28 -2.6
294,005 + H281DRAFT_04851 0.30 -1.1
294,066 - H281DRAFT_04851 0.33 -2.5
294,348 + H281DRAFT_04851 0.48 -1.3
294,431 + H281DRAFT_04851 0.53 -2.4

Or see this region's nucleotide sequence