Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04701 and H281DRAFT_04702 are separated by 25 nucleotides H281DRAFT_04702 and H281DRAFT_04703 are separated by 47 nucleotides H281DRAFT_04703 and H281DRAFT_04704 are separated by 29 nucleotides
H281DRAFT_04701: H281DRAFT_04701 - molecular chaperone DnaJ, at 113,530 to 114,672
_04701
H281DRAFT_04702: H281DRAFT_04702 - para-aminobenzoate synthetase / 4-amino-4-deoxychorismate lyase, at 114,698 to 116,587
_04702
H281DRAFT_04703: H281DRAFT_04703 - ketopantoate hydroxymethyltransferase, at 116,635 to 117,450
_04703
H281DRAFT_04704: H281DRAFT_04704 - Deoxyadenosine/deoxycytidine kinase, at 117,480 to 118,160
_04704
Position (kb)
114
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 114.680 kb on + strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.681 kb on - strand at 114.723 kb on + strand at 114.724 kb on - strand at 114.779 kb on + strand at 114.780 kb on + strand at 114.780 kb on - strand at 114.780 kb on - strand at 115.157 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.169 kb on + strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.170 kb on - strand, within H281DRAFT_04702 at 115.175 kb on + strand, within H281DRAFT_04702 at 115.195 kb on - strand, within H281DRAFT_04702 at 115.532 kb on + strand, within H281DRAFT_04702 at 115.700 kb on + strand, within H281DRAFT_04702 at 115.851 kb on + strand, within H281DRAFT_04702 at 115.852 kb on - strand, within H281DRAFT_04702 at 115.852 kb on - strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.964 kb on + strand, within H281DRAFT_04702 at 115.965 kb on - strand, within H281DRAFT_04702 at 116.038 kb on - strand, within H281DRAFT_04702 at 116.134 kb on - strand, within H281DRAFT_04702 at 116.250 kb on + strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.251 kb on - strand, within H281DRAFT_04702 at 116.329 kb on + strand, within H281DRAFT_04702 at 116.329 kb on + strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.330 kb on - strand, within H281DRAFT_04702 at 116.383 kb on - strand, within H281DRAFT_04702 at 116.476 kb on + strand at 116.476 kb on + strand at 116.477 kb on - strand at 116.517 kb on + strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.518 kb on - strand at 116.688 kb on - strand at 117.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 114,680 + -1.0 114,681 - +0.3 114,681 - -0.5 114,681 - -0.6 114,681 - +0.1 114,723 + +0.1 114,724 - -0.7 114,779 + +1.2 114,780 + +0.3 114,780 - -0.3 114,780 - +1.1 115,157 + H281DRAFT_04702 0.24 +0.3 115,169 + H281DRAFT_04702 0.25 +0.5 115,169 + H281DRAFT_04702 0.25 -0.6 115,169 + H281DRAFT_04702 0.25 +0.2 115,169 + H281DRAFT_04702 0.25 +0.9 115,169 + H281DRAFT_04702 0.25 -0.4 115,170 - H281DRAFT_04702 0.25 +0.5 115,170 - H281DRAFT_04702 0.25 +0.2 115,170 - H281DRAFT_04702 0.25 +1.0 115,175 + H281DRAFT_04702 0.25 +0.5 115,195 - H281DRAFT_04702 0.26 -0.5 115,532 + H281DRAFT_04702 0.44 -1.0 115,700 + H281DRAFT_04702 0.53 +2.9 115,851 + H281DRAFT_04702 0.61 -0.6 115,852 - H281DRAFT_04702 0.61 -1.2 115,852 - H281DRAFT_04702 0.61 +0.8 115,964 + H281DRAFT_04702 0.67 +1.3 115,964 + H281DRAFT_04702 0.67 +0.2 115,964 + H281DRAFT_04702 0.67 +0.5 115,965 - H281DRAFT_04702 0.67 +0.5 116,038 - H281DRAFT_04702 0.71 +0.5 116,134 - H281DRAFT_04702 0.76 +1.5 116,250 + H281DRAFT_04702 0.82 +0.0 116,251 - H281DRAFT_04702 0.82 -1.3 116,251 - H281DRAFT_04702 0.82 +0.5 116,251 - H281DRAFT_04702 0.82 +0.4 116,329 + H281DRAFT_04702 0.86 -0.2 116,329 + H281DRAFT_04702 0.86 -0.5 116,330 - H281DRAFT_04702 0.86 +0.5 116,330 - H281DRAFT_04702 0.86 +2.6 116,330 - H281DRAFT_04702 0.86 -0.6 116,383 - H281DRAFT_04702 0.89 -0.3 116,476 + +0.9 116,476 + -0.3 116,477 - +0.7 116,517 + -0.2 116,518 - +0.3 116,518 - +0.1 116,518 - +0.4 116,518 - +1.1 116,688 - +2.1 117,444 - -1.9
Or see this region's nucleotide sequence