Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04702

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04701 and H281DRAFT_04702 are separated by 25 nucleotidesH281DRAFT_04702 and H281DRAFT_04703 are separated by 47 nucleotidesH281DRAFT_04703 and H281DRAFT_04704 are separated by 29 nucleotides H281DRAFT_04701: H281DRAFT_04701 - molecular chaperone DnaJ, at 113,530 to 114,672 _04701 H281DRAFT_04702: H281DRAFT_04702 - para-aminobenzoate synthetase / 4-amino-4-deoxychorismate lyase, at 114,698 to 116,587 _04702 H281DRAFT_04703: H281DRAFT_04703 - ketopantoate hydroxymethyltransferase, at 116,635 to 117,450 _04703 H281DRAFT_04704: H281DRAFT_04704 - Deoxyadenosine/deoxycytidine kinase, at 117,480 to 118,160 _04704 Position (kb) 114 115 116 117Strain fitness (log2 ratio) -2 -1 0 1 2 3at 114.680 kb on + strandat 114.681 kb on - strandat 114.681 kb on - strandat 114.681 kb on - strandat 114.681 kb on - strandat 114.723 kb on + strandat 114.724 kb on - strandat 114.779 kb on + strandat 114.780 kb on + strandat 114.780 kb on - strandat 114.780 kb on - strandat 115.157 kb on + strand, within H281DRAFT_04702at 115.169 kb on + strand, within H281DRAFT_04702at 115.169 kb on + strand, within H281DRAFT_04702at 115.169 kb on + strand, within H281DRAFT_04702at 115.169 kb on + strand, within H281DRAFT_04702at 115.169 kb on + strand, within H281DRAFT_04702at 115.170 kb on - strand, within H281DRAFT_04702at 115.170 kb on - strand, within H281DRAFT_04702at 115.170 kb on - strand, within H281DRAFT_04702at 115.175 kb on + strand, within H281DRAFT_04702at 115.195 kb on - strand, within H281DRAFT_04702at 115.532 kb on + strand, within H281DRAFT_04702at 115.700 kb on + strand, within H281DRAFT_04702at 115.851 kb on + strand, within H281DRAFT_04702at 115.852 kb on - strand, within H281DRAFT_04702at 115.852 kb on - strand, within H281DRAFT_04702at 115.964 kb on + strand, within H281DRAFT_04702at 115.964 kb on + strand, within H281DRAFT_04702at 115.964 kb on + strand, within H281DRAFT_04702at 115.965 kb on - strand, within H281DRAFT_04702at 116.038 kb on - strand, within H281DRAFT_04702at 116.134 kb on - strand, within H281DRAFT_04702at 116.250 kb on + strand, within H281DRAFT_04702at 116.251 kb on - strand, within H281DRAFT_04702at 116.251 kb on - strand, within H281DRAFT_04702at 116.251 kb on - strand, within H281DRAFT_04702at 116.329 kb on + strand, within H281DRAFT_04702at 116.329 kb on + strand, within H281DRAFT_04702at 116.330 kb on - strand, within H281DRAFT_04702at 116.330 kb on - strand, within H281DRAFT_04702at 116.330 kb on - strand, within H281DRAFT_04702at 116.383 kb on - strand, within H281DRAFT_04702at 116.476 kb on + strandat 116.476 kb on + strandat 116.477 kb on - strandat 116.517 kb on + strandat 116.518 kb on - strandat 116.518 kb on - strandat 116.518 kb on - strandat 116.518 kb on - strandat 116.688 kb on - strandat 117.444 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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114,680 + -1.0
114,681 - +0.3
114,681 - -0.5
114,681 - -0.6
114,681 - +0.1
114,723 + +0.1
114,724 - -0.7
114,779 + +1.2
114,780 + +0.3
114,780 - -0.3
114,780 - +1.1
115,157 + H281DRAFT_04702 0.24 +0.3
115,169 + H281DRAFT_04702 0.25 +0.5
115,169 + H281DRAFT_04702 0.25 -0.6
115,169 + H281DRAFT_04702 0.25 +0.2
115,169 + H281DRAFT_04702 0.25 +0.9
115,169 + H281DRAFT_04702 0.25 -0.4
115,170 - H281DRAFT_04702 0.25 +0.5
115,170 - H281DRAFT_04702 0.25 +0.2
115,170 - H281DRAFT_04702 0.25 +1.0
115,175 + H281DRAFT_04702 0.25 +0.5
115,195 - H281DRAFT_04702 0.26 -0.5
115,532 + H281DRAFT_04702 0.44 -1.0
115,700 + H281DRAFT_04702 0.53 +2.9
115,851 + H281DRAFT_04702 0.61 -0.6
115,852 - H281DRAFT_04702 0.61 -1.2
115,852 - H281DRAFT_04702 0.61 +0.8
115,964 + H281DRAFT_04702 0.67 +1.3
115,964 + H281DRAFT_04702 0.67 +0.2
115,964 + H281DRAFT_04702 0.67 +0.5
115,965 - H281DRAFT_04702 0.67 +0.5
116,038 - H281DRAFT_04702 0.71 +0.5
116,134 - H281DRAFT_04702 0.76 +1.5
116,250 + H281DRAFT_04702 0.82 +0.0
116,251 - H281DRAFT_04702 0.82 -1.3
116,251 - H281DRAFT_04702 0.82 +0.5
116,251 - H281DRAFT_04702 0.82 +0.4
116,329 + H281DRAFT_04702 0.86 -0.2
116,329 + H281DRAFT_04702 0.86 -0.5
116,330 - H281DRAFT_04702 0.86 +0.5
116,330 - H281DRAFT_04702 0.86 +2.6
116,330 - H281DRAFT_04702 0.86 -0.6
116,383 - H281DRAFT_04702 0.89 -0.3
116,476 + +0.9
116,476 + -0.3
116,477 - +0.7
116,517 + -0.2
116,518 - +0.3
116,518 - +0.1
116,518 - +0.4
116,518 - +1.1
116,688 - +2.1
117,444 - -1.9

Or see this region's nucleotide sequence