Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04266 and H281DRAFT_04267 are separated by 122 nucleotides H281DRAFT_04267 and H281DRAFT_04268 overlap by 4 nucleotides H281DRAFT_04268 and H281DRAFT_04269 overlap by 1 nucleotides H281DRAFT_04269 and H281DRAFT_04270 are separated by 82 nucleotides
H281DRAFT_04266: H281DRAFT_04266 - glutamine amidotransferase, at 384,311 to 385,246
_04266
H281DRAFT_04267: H281DRAFT_04267 - L-glutamate ABC transporter ATP-binding protein /L-aspartate ABC transporter ATP-binding protein, at 385,369 to 386,094
_04267
H281DRAFT_04268: H281DRAFT_04268 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,091 to 386,768
_04268
H281DRAFT_04269: H281DRAFT_04269 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,768 to 387,508
_04269
H281DRAFT_04270: H281DRAFT_04270 - L-glutamate-binding protein /L-aspartate-binding protein, at 387,591 to 388,484
_04270
Position (kb) 386
387 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 385.288 kb on - strand at 386.048 kb on - strand at 386.086 kb on - strand at 386.086 kb on - strand at 386.086 kb on - strand at 386.175 kb on + strand, within H281DRAFT_04268 at 386.175 kb on + strand, within H281DRAFT_04268 at 386.251 kb on + strand, within H281DRAFT_04268 at 386.252 kb on - strand, within H281DRAFT_04268 at 386.264 kb on + strand, within H281DRAFT_04268 at 386.265 kb on - strand, within H281DRAFT_04268 at 386.265 kb on - strand, within H281DRAFT_04268 at 386.356 kb on + strand, within H281DRAFT_04268 at 386.357 kb on - strand, within H281DRAFT_04268 at 386.357 kb on - strand, within H281DRAFT_04268 at 386.435 kb on - strand, within H281DRAFT_04268 at 386.435 kb on - strand, within H281DRAFT_04268 at 386.438 kb on - strand, within H281DRAFT_04268 at 386.438 kb on - strand, within H281DRAFT_04268 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.782 kb on + strand at 386.784 kb on + strand at 386.853 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.884 kb on - strand, within H281DRAFT_04269 at 386.899 kb on - strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.243 kb on - strand, within H281DRAFT_04269 at 387.252 kb on + strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.444 kb on + strand at 387.444 kb on + strand at 387.445 kb on - strand at 387.445 kb on - strand at 387.501 kb on + strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.585 kb on + strand at 387.585 kb on + strand at 387.586 kb on - strand at 387.586 kb on - strand at 387.592 kb on + strand at 387.704 kb on - strand, within H281DRAFT_04270 at 387.704 kb on - strand, within H281DRAFT_04270
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 385,288 - -1.8 386,048 - +1.1 386,086 - +0.9 386,086 - +1.4 386,086 - +1.9 386,175 + H281DRAFT_04268 0.12 +1.1 386,175 + H281DRAFT_04268 0.12 +0.6 386,251 + H281DRAFT_04268 0.24 +0.2 386,252 - H281DRAFT_04268 0.24 +1.5 386,264 + H281DRAFT_04268 0.26 +0.2 386,265 - H281DRAFT_04268 0.26 +0.5 386,265 - H281DRAFT_04268 0.26 +1.7 386,356 + H281DRAFT_04268 0.39 +1.6 386,357 - H281DRAFT_04268 0.39 +1.3 386,357 - H281DRAFT_04268 0.39 +1.0 386,435 - H281DRAFT_04268 0.51 +0.3 386,435 - H281DRAFT_04268 0.51 +1.5 386,438 - H281DRAFT_04268 0.51 +1.7 386,438 - H281DRAFT_04268 0.51 +1.1 386,593 + H281DRAFT_04268 0.74 +0.2 386,593 + H281DRAFT_04268 0.74 +1.0 386,594 - H281DRAFT_04268 0.74 +1.6 386,594 - H281DRAFT_04268 0.74 +1.3 386,782 + +0.6 386,784 + +0.3 386,853 + H281DRAFT_04269 0.11 +1.0 386,883 + H281DRAFT_04269 0.16 +0.6 386,883 + H281DRAFT_04269 0.16 +1.2 386,884 - H281DRAFT_04269 0.16 +1.6 386,899 - H281DRAFT_04269 0.18 +1.2 386,974 + H281DRAFT_04269 0.28 +0.2 386,974 + H281DRAFT_04269 0.28 +2.7 386,974 + H281DRAFT_04269 0.28 +1.1 386,975 - H281DRAFT_04269 0.28 +0.8 386,975 - H281DRAFT_04269 0.28 +0.9 387,036 + H281DRAFT_04269 0.36 +1.7 387,036 + H281DRAFT_04269 0.36 +1.4 387,243 - H281DRAFT_04269 0.64 +3.2 387,252 + H281DRAFT_04269 0.65 +0.0 387,253 - H281DRAFT_04269 0.65 +1.5 387,253 - H281DRAFT_04269 0.65 +1.0 387,304 - H281DRAFT_04269 0.72 +0.9 387,304 - H281DRAFT_04269 0.72 +1.0 387,444 + +1.5 387,444 + +0.2 387,445 - +1.5 387,445 - +1.0 387,501 + +1.3 387,502 - +1.4 387,502 - +1.9 387,502 - +1.1 387,585 + +1.1 387,585 + +1.6 387,586 - +1.9 387,586 - +0.9 387,592 + +1.0 387,704 - H281DRAFT_04270 0.13 +2.0 387,704 - H281DRAFT_04270 0.13 +1.7
Or see this region's nucleotide sequence