Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04185 and H281DRAFT_04186 are separated by 156 nucleotides H281DRAFT_04186 and H281DRAFT_04187 are separated by 36 nucleotides H281DRAFT_04187 and H281DRAFT_04188 are separated by 142 nucleotides
H281DRAFT_04185: H281DRAFT_04185 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 303,611 to 304,807
_04185
H281DRAFT_04186: H281DRAFT_04186 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 304,964 to 305,482
_04186
H281DRAFT_04187: H281DRAFT_04187 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 305,519 to 306,409
_04187
H281DRAFT_04188: H281DRAFT_04188 - fructose-bisphosphate aldolase, at 306,552 to 307,616
_04188
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 305.179 kb on - strand, within H281DRAFT_04186 at 305.308 kb on - strand, within H281DRAFT_04186 at 305.403 kb on - strand, within H281DRAFT_04186 at 305.911 kb on - strand, within H281DRAFT_04187 at 306.340 kb on - strand at 306.340 kb on - strand at 306.526 kb on - strand at 306.546 kb on + strand at 306.546 kb on + strand at 306.586 kb on + strand at 306.586 kb on + strand at 306.587 kb on - strand at 306.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 305,179 - H281DRAFT_04186 0.41 +1.6 305,308 - H281DRAFT_04186 0.66 -3.1 305,403 - H281DRAFT_04186 0.85 -2.7 305,911 - H281DRAFT_04187 0.44 +1.1 306,340 - -0.1 306,340 - -1.4 306,526 - -0.2 306,546 + +0.3 306,546 + -0.8 306,586 + +0.6 306,586 + +0.4 306,587 - +0.9 306,587 - -0.8
Or see this region's nucleotide sequence