Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04178 and H281DRAFT_04179 are separated by 141 nucleotides H281DRAFT_04179 and H281DRAFT_04180 are separated by 4 nucleotides H281DRAFT_04180 and H281DRAFT_04181 are separated by 302 nucleotides
H281DRAFT_04178: H281DRAFT_04178 - serine protease Do, at 294,298 to 295,818
_04178
H281DRAFT_04179: H281DRAFT_04179 - two-component system, OmpR family, sensor kinase, at 295,960 to 297,261
_04179
H281DRAFT_04180: H281DRAFT_04180 - two component transcriptional regulator, winged helix family, at 297,266 to 297,928
_04180
H281DRAFT_04181: H281DRAFT_04181 - D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4), at 298,231 to 299,928
_04181
Position (kb) 295
296
297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 294.998 kb on + strand, within H281DRAFT_04178 at 295.100 kb on + strand, within H281DRAFT_04178 at 295.156 kb on - strand, within H281DRAFT_04178 at 295.381 kb on - strand, within H281DRAFT_04178 at 295.382 kb on + strand, within H281DRAFT_04178 at 295.383 kb on - strand, within H281DRAFT_04178 at 295.383 kb on - strand, within H281DRAFT_04178 at 295.383 kb on - strand, within H281DRAFT_04178 at 295.401 kb on + strand, within H281DRAFT_04178 at 295.401 kb on + strand, within H281DRAFT_04178 at 295.401 kb on + strand, within H281DRAFT_04178 at 295.493 kb on + strand, within H281DRAFT_04178 at 295.493 kb on + strand, within H281DRAFT_04178 at 295.494 kb on - strand, within H281DRAFT_04178 at 295.494 kb on - strand, within H281DRAFT_04178 at 295.502 kb on + strand, within H281DRAFT_04178 at 295.503 kb on - strand, within H281DRAFT_04178 at 295.823 kb on + strand at 295.888 kb on + strand at 295.888 kb on + strand at 295.888 kb on + strand at 295.889 kb on - strand at 295.974 kb on + strand at 295.975 kb on - strand at 296.017 kb on - strand at 296.083 kb on - strand at 296.240 kb on + strand, within H281DRAFT_04179 at 296.240 kb on + strand, within H281DRAFT_04179 at 296.241 kb on - strand, within H281DRAFT_04179 at 296.241 kb on - strand, within H281DRAFT_04179 at 296.367 kb on - strand, within H281DRAFT_04179 at 296.955 kb on + strand, within H281DRAFT_04179 at 297.015 kb on - strand, within H281DRAFT_04179 at 297.015 kb on - strand, within H281DRAFT_04179 at 297.018 kb on - strand, within H281DRAFT_04179 at 297.018 kb on - strand, within H281DRAFT_04179 at 297.018 kb on - strand, within H281DRAFT_04179 at 297.179 kb on + strand at 297.289 kb on - strand at 297.364 kb on + strand, within H281DRAFT_04180 at 297.364 kb on + strand, within H281DRAFT_04180 at 297.364 kb on + strand, within H281DRAFT_04180 at 297.364 kb on + strand, within H281DRAFT_04180 at 297.364 kb on + strand, within H281DRAFT_04180 at 297.365 kb on - strand, within H281DRAFT_04180 at 297.365 kb on - strand, within H281DRAFT_04180 at 297.365 kb on - strand, within H281DRAFT_04180 at 297.365 kb on - strand, within H281DRAFT_04180 at 297.393 kb on + strand, within H281DRAFT_04180 at 297.393 kb on + strand, within H281DRAFT_04180 at 297.394 kb on - strand, within H281DRAFT_04180 at 297.596 kb on - strand, within H281DRAFT_04180 at 297.636 kb on + strand, within H281DRAFT_04180 at 297.636 kb on + strand, within H281DRAFT_04180 at 297.743 kb on + strand, within H281DRAFT_04180 at 297.744 kb on - strand, within H281DRAFT_04180 at 297.966 kb on - strand at 298.030 kb on + strand at 298.030 kb on + strand at 298.031 kb on - strand at 298.031 kb on - strand at 298.077 kb on + strand at 298.077 kb on + strand at 298.077 kb on + strand at 298.080 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 294,998 + H281DRAFT_04178 0.46 +0.9 295,100 + H281DRAFT_04178 0.53 -3.7 295,156 - H281DRAFT_04178 0.56 -0.8 295,381 - H281DRAFT_04178 0.71 -1.2 295,382 + H281DRAFT_04178 0.71 +0.1 295,383 - H281DRAFT_04178 0.71 -0.8 295,383 - H281DRAFT_04178 0.71 -0.4 295,383 - H281DRAFT_04178 0.71 +0.6 295,401 + H281DRAFT_04178 0.73 +0.5 295,401 + H281DRAFT_04178 0.73 -0.9 295,401 + H281DRAFT_04178 0.73 -0.9 295,493 + H281DRAFT_04178 0.79 -0.8 295,493 + H281DRAFT_04178 0.79 -0.5 295,494 - H281DRAFT_04178 0.79 +1.2 295,494 - H281DRAFT_04178 0.79 +0.4 295,502 + H281DRAFT_04178 0.79 -0.6 295,503 - H281DRAFT_04178 0.79 -0.2 295,823 + -0.7 295,888 + -0.2 295,888 + +0.2 295,888 + +0.2 295,889 - -0.4 295,974 + +0.5 295,975 - -0.7 296,017 - +0.1 296,083 - -0.7 296,240 + H281DRAFT_04179 0.22 -0.1 296,240 + H281DRAFT_04179 0.22 -0.6 296,241 - H281DRAFT_04179 0.22 -1.3 296,241 - H281DRAFT_04179 0.22 -0.7 296,367 - H281DRAFT_04179 0.31 -0.9 296,955 + H281DRAFT_04179 0.76 -2.5 297,015 - H281DRAFT_04179 0.81 -0.8 297,015 - H281DRAFT_04179 0.81 -1.4 297,018 - H281DRAFT_04179 0.81 +0.1 297,018 - H281DRAFT_04179 0.81 -0.6 297,018 - H281DRAFT_04179 0.81 +1.1 297,179 + -0.2 297,289 - +0.1 297,364 + H281DRAFT_04180 0.15 +0.4 297,364 + H281DRAFT_04180 0.15 +0.3 297,364 + H281DRAFT_04180 0.15 +0.2 297,364 + H281DRAFT_04180 0.15 -0.6 297,364 + H281DRAFT_04180 0.15 +0.2 297,365 - H281DRAFT_04180 0.15 +0.7 297,365 - H281DRAFT_04180 0.15 -1.8 297,365 - H281DRAFT_04180 0.15 +0.5 297,365 - H281DRAFT_04180 0.15 +0.8 297,393 + H281DRAFT_04180 0.19 +1.6 297,393 + H281DRAFT_04180 0.19 +1.0 297,394 - H281DRAFT_04180 0.19 +0.3 297,596 - H281DRAFT_04180 0.50 +1.0 297,636 + H281DRAFT_04180 0.56 +0.6 297,636 + H281DRAFT_04180 0.56 +0.7 297,743 + H281DRAFT_04180 0.72 -0.5 297,744 - H281DRAFT_04180 0.72 +0.9 297,966 - +1.6 298,030 + +0.7 298,030 + -0.7 298,031 - -0.7 298,031 - -0.3 298,077 + -0.3 298,077 + -0.1 298,077 + -1.3 298,080 + -2.2
Or see this region's nucleotide sequence