Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04084 and H281DRAFT_04085 are separated by 295 nucleotides H281DRAFT_04085 and H281DRAFT_04086 are separated by 237 nucleotides
H281DRAFT_04084: H281DRAFT_04084 - cold-shock DNA-binding protein family, at 188,790 to 188,996
_04084
H281DRAFT_04085: H281DRAFT_04085 - isocitrate dehydrogenase (NADP), at 189,292 to 190,548
_04085
H281DRAFT_04086: H281DRAFT_04086 - 23S rRNA pseudouridine2457 synthase, at 190,786 to 191,448
_04086
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 188.383 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.603 kb on - strand at 188.636 kb on - strand at 188.774 kb on + strand at 188.775 kb on - strand at 189.089 kb on + strand at 189.090 kb on - strand at 189.188 kb on + strand at 189.293 kb on + strand at 189.293 kb on + strand at 189.294 kb on - strand at 189.371 kb on + strand at 189.444 kb on + strand, within H281DRAFT_04085 at 189.494 kb on + strand, within H281DRAFT_04085 at 189.494 kb on + strand, within H281DRAFT_04085 at 189.495 kb on - strand, within H281DRAFT_04085 at 189.509 kb on + strand, within H281DRAFT_04085 at 189.510 kb on - strand, within H281DRAFT_04085 at 189.561 kb on + strand, within H281DRAFT_04085 at 189.562 kb on - strand, within H281DRAFT_04085 at 189.620 kb on + strand, within H281DRAFT_04085 at 189.620 kb on + strand, within H281DRAFT_04085 at 189.621 kb on - strand, within H281DRAFT_04085 at 189.656 kb on + strand, within H281DRAFT_04085 at 189.656 kb on + strand, within H281DRAFT_04085 at 189.657 kb on - strand, within H281DRAFT_04085 at 189.813 kb on - strand, within H281DRAFT_04085 at 189.836 kb on + strand, within H281DRAFT_04085 at 189.951 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.065 kb on - strand, within H281DRAFT_04085 at 190.145 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.170 kb on - strand, within H281DRAFT_04085 at 190.170 kb on - strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.260 kb on - strand, within H281DRAFT_04085 at 190.260 kb on - strand, within H281DRAFT_04085 at 190.337 kb on + strand, within H281DRAFT_04085 at 190.373 kb on + strand, within H281DRAFT_04085 at 190.374 kb on - strand, within H281DRAFT_04085 at 190.419 kb on + strand, within H281DRAFT_04085 at 190.541 kb on + strand at 190.542 kb on - strand at 190.653 kb on - strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.751 kb on + strand at 190.778 kb on + strand at 190.779 kb on - strand at 190.786 kb on - strand at 190.786 kb on - strand at 190.803 kb on + strand at 190.804 kb on - strand at 190.804 kb on - strand at 190.878 kb on + strand, within H281DRAFT_04086 at 190.878 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.074 kb on - strand, within H281DRAFT_04086 at 191.164 kb on + strand, within H281DRAFT_04086 at 191.164 kb on + strand, within H281DRAFT_04086 at 191.165 kb on - strand, within H281DRAFT_04086 at 191.191 kb on + strand, within H281DRAFT_04086 at 191.209 kb on - strand, within H281DRAFT_04086 at 191.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 188,383 - -0.1 188,556 - +0.4 188,556 - +1.2 188,556 - -0.2 188,603 - -0.2 188,636 - -1.3 188,774 + -0.4 188,775 - -0.6 189,089 + -1.4 189,090 - -0.5 189,188 + +0.5 189,293 + +1.0 189,293 + +0.9 189,294 - +0.5 189,371 + +0.0 189,444 + H281DRAFT_04085 0.12 +0.8 189,494 + H281DRAFT_04085 0.16 +0.8 189,494 + H281DRAFT_04085 0.16 +0.1 189,495 - H281DRAFT_04085 0.16 +0.5 189,509 + H281DRAFT_04085 0.17 +0.4 189,510 - H281DRAFT_04085 0.17 +0.6 189,561 + H281DRAFT_04085 0.21 +0.8 189,562 - H281DRAFT_04085 0.21 -0.4 189,620 + H281DRAFT_04085 0.26 -0.6 189,620 + H281DRAFT_04085 0.26 -2.2 189,621 - H281DRAFT_04085 0.26 -1.3 189,656 + H281DRAFT_04085 0.29 -0.6 189,656 + H281DRAFT_04085 0.29 -1.5 189,657 - H281DRAFT_04085 0.29 -1.3 189,813 - H281DRAFT_04085 0.41 -0.2 189,836 + H281DRAFT_04085 0.43 +0.1 189,951 + H281DRAFT_04085 0.52 -0.7 190,064 + H281DRAFT_04085 0.61 +0.8 190,064 + H281DRAFT_04085 0.61 -0.0 190,064 + H281DRAFT_04085 0.61 +0.6 190,064 + H281DRAFT_04085 0.61 +0.3 190,065 - H281DRAFT_04085 0.61 +0.1 190,145 + H281DRAFT_04085 0.68 +0.5 190,169 + H281DRAFT_04085 0.70 -0.1 190,169 + H281DRAFT_04085 0.70 +0.5 190,169 + H281DRAFT_04085 0.70 -0.7 190,169 + H281DRAFT_04085 0.70 -0.3 190,169 + H281DRAFT_04085 0.70 -0.0 190,169 + H281DRAFT_04085 0.70 -2.4 190,169 + H281DRAFT_04085 0.70 +1.0 190,170 - H281DRAFT_04085 0.70 +0.4 190,170 - H281DRAFT_04085 0.70 -1.0 190,259 + H281DRAFT_04085 0.77 -0.3 190,259 + H281DRAFT_04085 0.77 -0.7 190,259 + H281DRAFT_04085 0.77 +0.3 190,259 + H281DRAFT_04085 0.77 +0.2 190,259 + H281DRAFT_04085 0.77 -0.3 190,260 - H281DRAFT_04085 0.77 -0.2 190,260 - H281DRAFT_04085 0.77 -0.0 190,337 + H281DRAFT_04085 0.83 +0.2 190,373 + H281DRAFT_04085 0.86 -0.4 190,374 - H281DRAFT_04085 0.86 -0.1 190,419 + H281DRAFT_04085 0.90 -1.7 190,541 + -0.1 190,542 - -2.8 190,653 - -0.4 190,747 + +0.9 190,747 + +0.4 190,747 + -0.2 190,747 + +0.2 190,751 + +0.4 190,778 + +0.3 190,779 - -1.0 190,786 - +2.7 190,786 - +0.0 190,803 + +0.2 190,804 - +0.5 190,804 - +0.9 190,878 + H281DRAFT_04086 0.14 +0.6 190,878 + H281DRAFT_04086 0.14 -0.4 191,073 + H281DRAFT_04086 0.43 +0.8 191,073 + H281DRAFT_04086 0.43 +0.4 191,073 + H281DRAFT_04086 0.43 +0.1 191,074 - H281DRAFT_04086 0.43 -0.0 191,164 + H281DRAFT_04086 0.57 +0.3 191,164 + H281DRAFT_04086 0.57 -0.3 191,165 - H281DRAFT_04086 0.57 +1.9 191,191 + H281DRAFT_04086 0.61 +0.0 191,209 - H281DRAFT_04086 0.64 +0.7 191,454 + -0.1
Or see this region's nucleotide sequence