Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03518 and H281DRAFT_03519 are separated by 439 nucleotides H281DRAFT_03519 and H281DRAFT_03520 are separated by 370 nucleotides H281DRAFT_03520 and H281DRAFT_03521 are separated by 129 nucleotides H281DRAFT_03521 and H281DRAFT_03522 are separated by 164 nucleotides
H281DRAFT_03518: H281DRAFT_03518 - hypothetical protein, at 18,611 to 18,916
_03518
H281DRAFT_03519: H281DRAFT_03519 - hypothetical protein, at 19,356 to 19,514
_03519
H281DRAFT_03520: H281DRAFT_03520 - hypothetical protein, at 19,885 to 21,225
_03520
H281DRAFT_03521: H281DRAFT_03521 - hypothetical protein, at 21,355 to 21,585
_03521
H281DRAFT_03522: H281DRAFT_03522 - Uncharacterized membrane protein, at 21,750 to 22,040
_03522
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.916 kb on + strand at 18.916 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 19.090 kb on - strand at 19.172 kb on - strand at 19.193 kb on + strand at 19.224 kb on + strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.227 kb on - strand at 19.354 kb on + strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.357 kb on + strand at 19.357 kb on + strand at 19.432 kb on + strand, within H281DRAFT_03519 at 19.438 kb on + strand, within H281DRAFT_03519 at 19.439 kb on - strand, within H281DRAFT_03519 at 19.531 kb on + strand at 19.532 kb on - strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.608 kb on - strand at 19.608 kb on - strand at 19.664 kb on - strand at 19.664 kb on - strand at 19.743 kb on + strand at 19.744 kb on - strand at 19.744 kb on - strand at 19.744 kb on - strand at 20.740 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.744 kb on - strand, within H281DRAFT_03520 at 20.744 kb on - strand, within H281DRAFT_03520 at 21.209 kb on - strand at 21.226 kb on - strand at 21.428 kb on + strand, within H281DRAFT_03521 at 21.457 kb on - strand at 21.457 kb on - strand at 21.689 kb on + strand at 21.690 kb on - strand at 21.882 kb on - strand, within H281DRAFT_03522 at 21.883 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.930 kb on - strand, within H281DRAFT_03522 at 21.930 kb on - strand, within H281DRAFT_03522 at 22.063 kb on + strand at 22.063 kb on + strand at 22.116 kb on + strand at 22.116 kb on + strand at 22.117 kb on - strand at 22.117 kb on - strand at 22.122 kb on - strand at 22.122 kb on - strand at 22.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 18,916 + -1.4 18,916 + +0.1 18,916 + -1.0 18,917 - -0.6 18,917 - +0.9 18,917 - -1.3 18,917 - +0.9 19,090 - -0.0 19,172 - +0.5 19,193 + +0.1 19,224 + -0.7 19,225 - -0.2 19,225 - -2.8 19,225 - +0.4 19,226 + +0.4 19,226 + +0.5 19,226 + +0.2 19,227 - -0.0 19,354 + -0.4 19,355 - -2.0 19,355 - +1.3 19,355 - +0.2 19,355 - -1.0 19,357 + +0.3 19,357 + +0.3 19,432 + H281DRAFT_03519 0.48 +0.5 19,438 + H281DRAFT_03519 0.52 +0.3 19,439 - H281DRAFT_03519 0.52 -1.0 19,531 + +0.1 19,532 - -0.8 19,607 + -0.4 19,607 + +1.9 19,607 + +0.2 19,607 + -0.8 19,608 - +1.1 19,608 - +0.3 19,664 - -1.5 19,664 - +0.5 19,743 + +0.2 19,744 - -0.6 19,744 - +2.1 19,744 - +0.6 20,740 + H281DRAFT_03520 0.64 +0.7 20,743 + H281DRAFT_03520 0.64 +0.5 20,743 + H281DRAFT_03520 0.64 -2.9 20,743 + H281DRAFT_03520 0.64 -0.5 20,743 + H281DRAFT_03520 0.64 -0.1 20,744 - H281DRAFT_03520 0.64 +1.2 20,744 - H281DRAFT_03520 0.64 -0.1 21,209 - -0.7 21,226 - +0.1 21,428 + H281DRAFT_03521 0.32 -0.0 21,457 - +1.9 21,457 - +0.3 21,689 + +1.0 21,690 - -0.8 21,882 - H281DRAFT_03522 0.45 +0.6 21,883 + H281DRAFT_03522 0.46 -0.5 21,921 + H281DRAFT_03522 0.59 -0.5 21,921 + H281DRAFT_03522 0.59 +1.4 21,921 + H281DRAFT_03522 0.59 -0.6 21,922 - H281DRAFT_03522 0.59 -0.9 21,922 - H281DRAFT_03522 0.59 -0.3 21,922 - H281DRAFT_03522 0.59 -2.4 21,929 + H281DRAFT_03522 0.62 +0.1 21,929 + H281DRAFT_03522 0.62 +0.2 21,929 + H281DRAFT_03522 0.62 -0.7 21,929 + H281DRAFT_03522 0.62 +0.1 21,929 + H281DRAFT_03522 0.62 -0.5 21,929 + H281DRAFT_03522 0.62 -0.4 21,930 - H281DRAFT_03522 0.62 -0.9 21,930 - H281DRAFT_03522 0.62 +1.1 22,063 + -0.2 22,063 + +0.1 22,116 + +0.3 22,116 + -1.1 22,117 - -0.2 22,117 - -0.4 22,122 - -0.4 22,122 - -0.2 22,225 + +0.8
Or see this region's nucleotide sequence