Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02101

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02100 and H281DRAFT_02101 are separated by 76 nucleotidesH281DRAFT_02101 and H281DRAFT_02102 are separated by 61 nucleotidesH281DRAFT_02102 and H281DRAFT_02103 are separated by 4 nucleotidesH281DRAFT_02103 and H281DRAFT_02104 overlap by 4 nucleotides H281DRAFT_02100: H281DRAFT_02100 - glyoxylate/hydroxypyruvate reductase A, at 226,953 to 227,894 _02100 H281DRAFT_02101: H281DRAFT_02101 - hydroxymethylglutaryl-CoA lyase, at 227,971 to 228,897 _02101 H281DRAFT_02102: H281DRAFT_02102 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 228,959 to 229,480 _02102 H281DRAFT_02103: H281DRAFT_02103 - hypothetical protein, at 229,485 to 229,712 _02103 H281DRAFT_02104: H281DRAFT_02104 - Acyl dehydratase, at 229,709 to 230,194 _02104 Position (kb) 227 228 229Strain fitness (log2 ratio) -3 -2 -1 0 1at 227.083 kb on + strand, within H281DRAFT_02100at 227.084 kb on - strand, within H281DRAFT_02100at 227.260 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.283 kb on + strand, within H281DRAFT_02100at 227.284 kb on - strand, within H281DRAFT_02100at 227.304 kb on + strand, within H281DRAFT_02100at 227.339 kb on - strand, within H281DRAFT_02100at 227.339 kb on - strand, within H281DRAFT_02100at 227.449 kb on - strand, within H281DRAFT_02100at 227.450 kb on + strand, within H281DRAFT_02100at 227.451 kb on - strand, within H281DRAFT_02100at 227.451 kb on - strand, within H281DRAFT_02100at 227.483 kb on + strand, within H281DRAFT_02100at 227.484 kb on - strand, within H281DRAFT_02100at 227.616 kb on + strand, within H281DRAFT_02100at 227.616 kb on + strand, within H281DRAFT_02100at 227.617 kb on - strand, within H281DRAFT_02100at 227.617 kb on - strand, within H281DRAFT_02100at 227.771 kb on + strand, within H281DRAFT_02100at 227.772 kb on - strand, within H281DRAFT_02100at 227.949 kb on + strandat 227.949 kb on + strandat 227.950 kb on - strandat 227.956 kb on + strandat 227.956 kb on + strandat 227.957 kb on - strandat 227.957 kb on - strandat 227.957 kb on - strandat 228.200 kb on - strandat 228.419 kb on - strand, within H281DRAFT_02101at 228.419 kb on - strand, within H281DRAFT_02101at 228.504 kb on + strand, within H281DRAFT_02101at 228.604 kb on + strand, within H281DRAFT_02101at 228.628 kb on + strand, within H281DRAFT_02101at 228.659 kb on - strand, within H281DRAFT_02101at 228.695 kb on - strand, within H281DRAFT_02101at 228.739 kb on + strand, within H281DRAFT_02101at 228.739 kb on + strand, within H281DRAFT_02101at 228.901 kb on + strandat 228.902 kb on - strandat 228.942 kb on + strandat 229.225 kb on - strand, within H281DRAFT_02102at 229.287 kb on - strand, within H281DRAFT_02102at 229.287 kb on - strand, within H281DRAFT_02102at 229.624 kb on - strand, within H281DRAFT_02103at 229.838 kb on + strand, within H281DRAFT_02104at 229.839 kb on - strand, within H281DRAFT_02104

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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227,083 + H281DRAFT_02100 0.14 +0.8
227,084 - H281DRAFT_02100 0.14 +0.1
227,260 - H281DRAFT_02100 0.33 +0.6
227,264 - H281DRAFT_02100 0.33 +0.8
227,264 - H281DRAFT_02100 0.33 -0.3
227,264 - H281DRAFT_02100 0.33 +1.3
227,283 + H281DRAFT_02100 0.35 -0.7
227,284 - H281DRAFT_02100 0.35 -0.1
227,304 + H281DRAFT_02100 0.37 -0.1
227,339 - H281DRAFT_02100 0.41 +0.1
227,339 - H281DRAFT_02100 0.41 +0.2
227,449 - H281DRAFT_02100 0.53 +0.2
227,450 + H281DRAFT_02100 0.53 +0.5
227,451 - H281DRAFT_02100 0.53 +0.4
227,451 - H281DRAFT_02100 0.53 -1.5
227,483 + H281DRAFT_02100 0.56 +0.7
227,484 - H281DRAFT_02100 0.56 +0.9
227,616 + H281DRAFT_02100 0.70 +0.0
227,616 + H281DRAFT_02100 0.70 -0.4
227,617 - H281DRAFT_02100 0.70 +0.1
227,617 - H281DRAFT_02100 0.70 +0.6
227,771 + H281DRAFT_02100 0.87 -0.2
227,772 - H281DRAFT_02100 0.87 +0.1
227,949 + -3.3
227,949 + -0.1
227,950 - -0.2
227,956 + +0.4
227,956 + -0.9
227,957 - +0.0
227,957 - -1.3
227,957 - +0.4
228,200 - +1.0
228,419 - H281DRAFT_02101 0.48 -0.6
228,419 - H281DRAFT_02101 0.48 -0.3
228,504 + H281DRAFT_02101 0.57 -0.7
228,604 + H281DRAFT_02101 0.68 +1.3
228,628 + H281DRAFT_02101 0.71 -1.2
228,659 - H281DRAFT_02101 0.74 -0.4
228,695 - H281DRAFT_02101 0.78 +0.1
228,739 + H281DRAFT_02101 0.83 -1.2
228,739 + H281DRAFT_02101 0.83 -1.1
228,901 + +0.2
228,902 - -0.9
228,942 + -0.6
229,225 - H281DRAFT_02102 0.51 +0.4
229,287 - H281DRAFT_02102 0.63 +0.7
229,287 - H281DRAFT_02102 0.63 +0.5
229,624 - H281DRAFT_02103 0.61 -2.5
229,838 + H281DRAFT_02104 0.27 -0.1
229,839 - H281DRAFT_02104 0.27 +0.4

Or see this region's nucleotide sequence