Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02007

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02005 and H281DRAFT_02006 are separated by 74 nucleotidesH281DRAFT_02006 and H281DRAFT_02007 are separated by 72 nucleotidesH281DRAFT_02007 and H281DRAFT_02008 are separated by 248 nucleotidesH281DRAFT_02008 and H281DRAFT_02009 are separated by 80 nucleotides H281DRAFT_02005: H281DRAFT_02005 - carboxyl-terminal processing protease, at 121,436 to 123,013 _02005 H281DRAFT_02006: H281DRAFT_02006 - Molybdopterin or thiamine biosynthesis adenylyltransferase, at 123,088 to 123,846 _02006 H281DRAFT_02007: H281DRAFT_02007 - phosphoenolpyruvate--protein phosphotransferase, at 123,919 to 125,667 _02007 H281DRAFT_02008: H281DRAFT_02008 - phosphocarrier protein, at 125,916 to 126,185 _02008 H281DRAFT_02009: H281DRAFT_02009 - PTS system, ascorbate-specific IIA component, at 126,266 to 126,754 _02009 Position (kb) 123 124 125 126Strain fitness (log2 ratio) -2 -1 0 1 2 3at 123.011 kb on + strandat 123.011 kb on + strandat 123.011 kb on + strandat 123.011 kb on + strandat 123.011 kb on + strandat 123.940 kb on - strandat 123.940 kb on - strandat 124.215 kb on - strand, within H281DRAFT_02007at 124.259 kb on + strand, within H281DRAFT_02007at 124.260 kb on - strand, within H281DRAFT_02007at 124.376 kb on + strand, within H281DRAFT_02007at 124.376 kb on + strand, within H281DRAFT_02007at 124.377 kb on - strand, within H281DRAFT_02007at 124.596 kb on - strand, within H281DRAFT_02007at 124.639 kb on + strand, within H281DRAFT_02007at 124.846 kb on - strand, within H281DRAFT_02007at 124.934 kb on + strand, within H281DRAFT_02007at 124.934 kb on + strand, within H281DRAFT_02007at 124.934 kb on + strand, within H281DRAFT_02007at 124.934 kb on + strandat 124.935 kb on - strand, within H281DRAFT_02007at 124.935 kb on - strand, within H281DRAFT_02007at 124.935 kb on - strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.082 kb on + strand, within H281DRAFT_02007at 125.083 kb on - strand, within H281DRAFT_02007at 125.092 kb on - strand, within H281DRAFT_02007at 125.092 kb on - strand, within H281DRAFT_02007at 125.092 kb on - strand, within H281DRAFT_02007at 125.092 kb on - strand, within H281DRAFT_02007at 125.092 kb on - strand, within H281DRAFT_02007at 125.136 kb on + strand, within H281DRAFT_02007at 125.137 kb on - strand, within H281DRAFT_02007at 125.137 kb on - strand, within H281DRAFT_02007at 125.294 kb on + strand, within H281DRAFT_02007at 125.294 kb on + strand, within H281DRAFT_02007at 125.366 kb on + strand, within H281DRAFT_02007at 125.367 kb on - strand, within H281DRAFT_02007at 125.434 kb on + strand, within H281DRAFT_02007at 125.434 kb on + strand, within H281DRAFT_02007at 125.434 kb on + strand, within H281DRAFT_02007at 125.435 kb on - strand, within H281DRAFT_02007at 125.435 kb on - strand, within H281DRAFT_02007at 125.435 kb on - strand, within H281DRAFT_02007at 125.574 kb on - strandat 125.574 kb on - strandat 125.581 kb on + strandat 125.581 kb on + strandat 125.581 kb on + strandat 125.582 kb on - strandat 125.609 kb on + strandat 125.609 kb on + strandat 125.609 kb on + strandat 125.609 kb on + strandat 125.610 kb on - strandat 125.610 kb on - strandat 125.629 kb on - strandat 125.680 kb on + strandat 125.680 kb on + strandat 125.680 kb on + strandat 125.680 kb on + strandat 125.681 kb on - strandat 125.681 kb on - strandat 125.681 kb on - strandat 125.736 kb on - strandat 125.737 kb on + strandat 125.738 kb on - strandat 125.797 kb on + strandat 125.844 kb on + strandat 125.899 kb on + strandat 125.899 kb on + strandat 125.899 kb on + strandat 125.899 kb on + strandat 125.900 kb on - strandat 125.937 kb on - strandat 126.045 kb on - strand, within H281DRAFT_02008at 126.062 kb on + strand, within H281DRAFT_02008at 126.100 kb on + strand, within H281DRAFT_02008at 126.100 kb on + strand, within H281DRAFT_02008at 126.100 kb on + strand, within H281DRAFT_02008at 126.100 kb on + strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.101 kb on - strand, within H281DRAFT_02008at 126.164 kb on + strandat 126.206 kb on + strandat 126.206 kb on + strandat 126.207 kb on - strandat 126.207 kb on - strandat 126.240 kb on + strandat 126.241 kb on - strandat 126.241 kb on - strandat 126.241 kb on - strandat 126.244 kb on + strandat 126.245 kb on - strandat 126.245 kb on - strandat 126.245 kb on - strandat 126.245 kb on - strandat 126.245 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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123,011 + +0.1
123,011 + +1.4
123,011 + +0.4
123,011 + +0.2
123,011 + +1.0
123,940 - +1.3
123,940 - +0.1
124,215 - H281DRAFT_02007 0.17 +0.5
124,259 + H281DRAFT_02007 0.19 +0.4
124,260 - H281DRAFT_02007 0.19 +0.9
124,376 + H281DRAFT_02007 0.26 +0.2
124,376 + H281DRAFT_02007 0.26 +0.7
124,377 - H281DRAFT_02007 0.26 +1.0
124,596 - H281DRAFT_02007 0.39 +0.5
124,639 + H281DRAFT_02007 0.41 +1.0
124,846 - H281DRAFT_02007 0.53 +1.1
124,934 + H281DRAFT_02007 0.58 +1.0
124,934 + H281DRAFT_02007 0.58 +1.5
124,934 + H281DRAFT_02007 0.58 +0.9
124,934 + +3.3
124,935 - H281DRAFT_02007 0.58 +0.6
124,935 - H281DRAFT_02007 0.58 +1.0
124,935 - H281DRAFT_02007 0.58 +1.0
125,082 + H281DRAFT_02007 0.66 +0.5
125,082 + H281DRAFT_02007 0.66 +3.3
125,082 + H281DRAFT_02007 0.66 +1.5
125,082 + H281DRAFT_02007 0.66 +1.5
125,082 + H281DRAFT_02007 0.66 +0.8
125,082 + H281DRAFT_02007 0.66 +1.2
125,082 + H281DRAFT_02007 0.66 -0.7
125,083 - H281DRAFT_02007 0.67 -0.0
125,092 - H281DRAFT_02007 0.67 +1.1
125,092 - H281DRAFT_02007 0.67 +0.9
125,092 - H281DRAFT_02007 0.67 +0.9
125,092 - H281DRAFT_02007 0.67 +0.1
125,092 - H281DRAFT_02007 0.67 +0.7
125,136 + H281DRAFT_02007 0.70 +1.7
125,137 - H281DRAFT_02007 0.70 -0.0
125,137 - H281DRAFT_02007 0.70 +1.3
125,294 + H281DRAFT_02007 0.79 +1.6
125,294 + H281DRAFT_02007 0.79 +0.4
125,366 + H281DRAFT_02007 0.83 +0.6
125,367 - H281DRAFT_02007 0.83 +0.8
125,434 + H281DRAFT_02007 0.87 +0.9
125,434 + H281DRAFT_02007 0.87 +0.7
125,434 + H281DRAFT_02007 0.87 +0.2
125,435 - H281DRAFT_02007 0.87 +1.6
125,435 - H281DRAFT_02007 0.87 +0.9
125,435 - H281DRAFT_02007 0.87 +1.0
125,574 - -0.0
125,574 - +1.0
125,581 + +1.1
125,581 + -0.5
125,581 + +0.6
125,582 - +0.2
125,609 + +0.6
125,609 + -0.0
125,609 + +0.8
125,609 + +1.0
125,610 - +2.6
125,610 - -0.6
125,629 - +0.4
125,680 + +0.8
125,680 + -0.6
125,680 + -0.4
125,680 + +0.6
125,681 - +0.8
125,681 - -1.0
125,681 - -1.0
125,736 - +0.0
125,737 + +1.0
125,738 - -0.1
125,797 + +0.2
125,844 + +0.3
125,899 + +0.9
125,899 + +0.5
125,899 + -0.7
125,899 + +2.0
125,900 - -1.8
125,937 - -0.9
126,045 - H281DRAFT_02008 0.48 +0.6
126,062 + H281DRAFT_02008 0.54 -0.6
126,100 + H281DRAFT_02008 0.68 -0.5
126,100 + H281DRAFT_02008 0.68 -0.0
126,100 + H281DRAFT_02008 0.68 -1.7
126,100 + H281DRAFT_02008 0.68 +0.4
126,101 - H281DRAFT_02008 0.69 +0.3
126,101 - H281DRAFT_02008 0.69 -0.3
126,101 - H281DRAFT_02008 0.69 -0.2
126,101 - H281DRAFT_02008 0.69 -0.6
126,101 - H281DRAFT_02008 0.69 -0.6
126,101 - H281DRAFT_02008 0.69 +1.2
126,164 + -1.4
126,206 + +0.1
126,206 + -2.2
126,207 - -1.6
126,207 - +0.4
126,240 + -0.1
126,241 - -0.3
126,241 - -0.0
126,241 - -1.0
126,244 + +0.3
126,245 - -1.3
126,245 - +2.0
126,245 - -0.8
126,245 - -1.4
126,245 - -0.6

Or see this region's nucleotide sequence