Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00378

Experiment: 2'-Deoxyinosine 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00377 and H281DRAFT_00378 are separated by 167 nucleotidesH281DRAFT_00378 and H281DRAFT_00379 are separated by 60 nucleotides H281DRAFT_00377: H281DRAFT_00377 - N-carbamoyl-L-amino-acid hydrolase, at 415,106 to 416,386 _00377 H281DRAFT_00378: H281DRAFT_00378 - glutamate synthase (NADPH/NADH) small chain, at 416,554 to 417,903 _00378 H281DRAFT_00379: H281DRAFT_00379 - dihydroorotate oxidase B, catalytic subunit /dihydrouracil dehydrogenase (NAD+) /dihydropyrimidine dehydrogenase (NADP+), at 417,964 to 419,292 _00379 Position (kb) 416 417 418Strain fitness (log2 ratio) -1 0 1at 416.228 kb on + strand, within H281DRAFT_00377at 416.341 kb on - strandat 416.554 kb on - strandat 416.689 kb on + strand, within H281DRAFT_00378at 417.112 kb on + strand, within H281DRAFT_00378

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C)
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416,228 + H281DRAFT_00377 0.88 -0.1
416,341 - -0.5
416,554 - +0.4
416,689 + H281DRAFT_00378 0.10 +1.3
417,112 + H281DRAFT_00378 0.41 -1.0

Or see this region's nucleotide sequence