Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15720 and CSW01_15725 overlap by 5 nucleotides CSW01_15725 and CSW01_15730 are separated by 73 nucleotides CSW01_15730 and CSW01_15735 are separated by 272 nucleotides CSW01_15735 and CSW01_15740 are separated by 267 nucleotides CSW01_15740 and CSW01_15745 are separated by 144 nucleotides
CSW01_15720: CSW01_15720 - LD-carboxypeptidase, at 334,211 to 335,215
_15720
CSW01_15725: CSW01_15725 - hypothetical protein, at 335,211 to 335,294
_15725
CSW01_15730: CSW01_15730 - VOC family protein, at 335,368 to 335,727
_15730
CSW01_15735: CSW01_15735 - DUF3709 domain-containing protein, at 336,000 to 336,233
_15735
CSW01_15740: CSW01_15740 - hypothetical protein, at 336,501 to 337,007
_15740
CSW01_15745: CSW01_15745 - VOC family virulence protein, at 337,152 to 337,550
_15745
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1 at 335.503 kb on + strand, within CSW01_15730 at 336.409 kb on + strand at 336.488 kb on - strand at 336.614 kb on + strand, within CSW01_15740 at 336.630 kb on + strand, within CSW01_15740 at 336.685 kb on + strand, within CSW01_15740 at 336.773 kb on + strand, within CSW01_15740 at 336.781 kb on - strand, within CSW01_15740 at 336.781 kb on - strand, within CSW01_15740 at 336.848 kb on - strand, within CSW01_15740 at 336.954 kb on + strand, within CSW01_15740 at 336.954 kb on + strand, within CSW01_15740 at 336.961 kb on + strand at 337.030 kb on - strand at 337.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 335,503 + CSW01_15730 0.38 -0.2 336,409 + -2.2 336,488 - +1.7 336,614 + CSW01_15740 0.22 -0.5 336,630 + CSW01_15740 0.25 -0.3 336,685 + CSW01_15740 0.36 -2.6 336,773 + CSW01_15740 0.54 -0.1 336,781 - CSW01_15740 0.55 -1.5 336,781 - CSW01_15740 0.55 +0.4 336,848 - CSW01_15740 0.68 -0.8 336,954 + CSW01_15740 0.89 -0.2 336,954 + CSW01_15740 0.89 -1.1 336,961 + +0.8 337,030 - -1.5 337,030 - -0.3
Or see this region's nucleotide sequence