Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15490 and CSW01_15495 are separated by 295 nucleotides CSW01_15495 and CSW01_15500 are separated by 353 nucleotides CSW01_15500 and CSW01_15505 are separated by 194 nucleotides
CSW01_15490: CSW01_15490 - hypothetical protein, at 315,690 to 316,094
_15490
CSW01_15495: CSW01_15495 - DUF645 domain-containing protein, at 316,390 to 316,572
_15495
CSW01_15500: CSW01_15500 - DUF645 domain-containing protein, at 316,926 to 317,108
_15500
CSW01_15505: CSW01_15505 - HNH endonuclease, at 317,303 to 317,980
_15505
Position (kb) 316
317
318 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 316.315 kb on + strand at 316.361 kb on + strand at 316.393 kb on - strand at 316.400 kb on - strand at 316.861 kb on + strand at 317.016 kb on - strand, within CSW01_15500 at 317.526 kb on + strand, within CSW01_15505 at 317.526 kb on + strand, within CSW01_15505 at 317.534 kb on - strand, within CSW01_15505 at 317.588 kb on - strand, within CSW01_15505 at 317.588 kb on - strand, within CSW01_15505 at 317.671 kb on - strand, within CSW01_15505 at 317.690 kb on + strand, within CSW01_15505 at 317.698 kb on - strand, within CSW01_15505 at 317.698 kb on - strand, within CSW01_15505 at 317.698 kb on - strand, within CSW01_15505 at 317.777 kb on + strand, within CSW01_15505 at 317.792 kb on + strand, within CSW01_15505 at 317.792 kb on + strand, within CSW01_15505
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 316,315 + -0.4 316,361 + +0.8 316,393 - -0.3 316,400 - -1.2 316,861 + +0.4 317,016 - CSW01_15500 0.49 +0.1 317,526 + CSW01_15505 0.33 +0.9 317,526 + CSW01_15505 0.33 +1.0 317,534 - CSW01_15505 0.34 +0.1 317,588 - CSW01_15505 0.42 -4.2 317,588 - CSW01_15505 0.42 -2.9 317,671 - CSW01_15505 0.54 -0.6 317,690 + CSW01_15505 0.57 -0.2 317,698 - CSW01_15505 0.58 +0.2 317,698 - CSW01_15505 0.58 -0.2 317,698 - CSW01_15505 0.58 -0.2 317,777 + CSW01_15505 0.70 +0.2 317,792 + CSW01_15505 0.72 -2.1 317,792 + CSW01_15505 0.72 +0.8
Or see this region's nucleotide sequence