Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15435 and CSW01_15440 are separated by 60 nucleotides CSW01_15440 and CSW01_15445 are separated by 255 nucleotides CSW01_15445 and CSW01_15450 overlap by 28 nucleotides CSW01_15450 and CSW01_15455 are separated by 18 nucleotides CSW01_15455 and CSW01_15460 are separated by 150 nucleotides CSW01_15460 and CSW01_15465 overlap by 44 nucleotides CSW01_15465 and CSW01_15470 are separated by 76 nucleotides
CSW01_15435: CSW01_15435 - general secretion pathway protein GspI, at 311,905 to 312,006
_15435
CSW01_15440: CSW01_15440 - DUF645 domain-containing protein, at 312,067 to 312,249
_15440
CSW01_15445: CSW01_15445 - TIGR03643 family protein, at 312,505 to 312,756
_15445
CSW01_15450: CSW01_15450 - DUF3265 domain-containing protein, at 312,729 to 312,878
_15450
CSW01_15455: CSW01_15455 - hypothetical protein, at 312,897 to 313,340
_15455
CSW01_15460: CSW01_15460 - type B-5 chloramphenicol O-acetyltransferase CatB9, at 313,491 to 314,120
_15460
CSW01_15465: CSW01_15465 - hypothetical protein, at 314,077 to 314,199
_15465
CSW01_15470: CSW01_15470 - NAD(P)-dependent oxidoreductase, at 314,276 to 315,028
_15470
Position (kb) 312
313
314 Strain fitness (log2 ratio) -2
-1
0
1
2 at 312.871 kb on + strand at 312.871 kb on + strand at 312.871 kb on + strand at 312.871 kb on + strand at 312.992 kb on + strand, within CSW01_15455 at 312.992 kb on + strand, within CSW01_15455 at 313.140 kb on - strand, within CSW01_15455 at 313.145 kb on - strand, within CSW01_15455 at 313.145 kb on - strand, within CSW01_15455 at 313.176 kb on + strand, within CSW01_15455 at 313.201 kb on - strand, within CSW01_15455 at 313.212 kb on - strand, within CSW01_15455 at 313.249 kb on + strand, within CSW01_15455 at 313.267 kb on - strand, within CSW01_15455 at 313.319 kb on - strand at 313.603 kb on + strand, within CSW01_15460 at 313.677 kb on - strand, within CSW01_15460 at 313.731 kb on + strand, within CSW01_15460 at 313.825 kb on + strand, within CSW01_15460 at 314.127 kb on - strand, within CSW01_15465 at 314.222 kb on + strand at 314.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 312,871 + -1.3 312,871 + +0.4 312,871 + -0.2 312,871 + +0.9 312,992 + CSW01_15455 0.21 +0.7 312,992 + CSW01_15455 0.21 -0.9 313,140 - CSW01_15455 0.55 -2.4 313,145 - CSW01_15455 0.56 -0.0 313,145 - CSW01_15455 0.56 -1.3 313,176 + CSW01_15455 0.63 +0.7 313,201 - CSW01_15455 0.68 -1.7 313,212 - CSW01_15455 0.71 +1.8 313,249 + CSW01_15455 0.79 +0.5 313,267 - CSW01_15455 0.83 +1.2 313,319 - +0.4 313,603 + CSW01_15460 0.18 -0.3 313,677 - CSW01_15460 0.30 +1.1 313,731 + CSW01_15460 0.38 -0.6 313,825 + CSW01_15460 0.53 -1.6 314,127 - CSW01_15465 0.41 -2.2 314,222 + -0.0 314,276 - -0.6
Or see this region's nucleotide sequence