Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15210 and CSW01_15215 are separated by 99 nucleotides CSW01_15215 and CSW01_15220 are separated by 172 nucleotides
CSW01_15210: CSW01_15210 - L-ascorbate 6-phosphate lactonase, at 268,323 to 269,390
_15210
CSW01_15215: CSW01_15215 - cytochrome b, at 269,490 to 270,023
_15215
CSW01_15220: CSW01_15220 - alpha-amylase, at 270,196 to 271,596
_15220
Position (kb) 269
270
271 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 268.559 kb on + strand, within CSW01_15210 at 268.582 kb on + strand, within CSW01_15210 at 268.590 kb on - strand, within CSW01_15210 at 268.667 kb on - strand, within CSW01_15210 at 268.671 kb on + strand, within CSW01_15210 at 268.788 kb on + strand, within CSW01_15210 at 268.807 kb on - strand, within CSW01_15210 at 268.881 kb on - strand, within CSW01_15210 at 268.936 kb on + strand, within CSW01_15210 at 268.936 kb on + strand, within CSW01_15210 at 268.944 kb on - strand, within CSW01_15210 at 268.986 kb on + strand, within CSW01_15210 at 268.994 kb on - strand, within CSW01_15210 at 268.994 kb on - strand, within CSW01_15210 at 269.028 kb on + strand, within CSW01_15210 at 269.055 kb on - strand, within CSW01_15210 at 269.128 kb on + strand, within CSW01_15210 at 269.391 kb on + strand at 269.468 kb on - strand at 269.481 kb on - strand at 269.486 kb on + strand at 269.513 kb on - strand at 269.518 kb on + strand at 269.622 kb on - strand, within CSW01_15215 at 269.658 kb on - strand, within CSW01_15215 at 269.660 kb on + strand, within CSW01_15215 at 269.675 kb on - strand, within CSW01_15215 at 269.683 kb on - strand, within CSW01_15215 at 269.867 kb on - strand, within CSW01_15215 at 269.875 kb on + strand, within CSW01_15215 at 269.875 kb on + strand, within CSW01_15215 at 269.883 kb on - strand, within CSW01_15215 at 269.930 kb on + strand, within CSW01_15215 at 269.982 kb on - strand at 269.992 kb on - strand at 270.112 kb on + strand at 270.201 kb on - strand at 270.201 kb on - strand at 270.203 kb on + strand at 270.266 kb on - strand at 270.266 kb on - strand at 270.292 kb on + strand at 270.405 kb on - strand, within CSW01_15220 at 270.555 kb on + strand, within CSW01_15220 at 270.563 kb on - strand, within CSW01_15220 at 270.711 kb on - strand, within CSW01_15220 at 270.805 kb on - strand, within CSW01_15220 at 270.807 kb on + strand, within CSW01_15220 at 270.824 kb on + strand, within CSW01_15220 at 270.825 kb on + strand, within CSW01_15220 at 270.841 kb on + strand, within CSW01_15220 at 270.841 kb on + strand, within CSW01_15220 at 270.907 kb on + strand, within CSW01_15220 at 270.915 kb on - strand, within CSW01_15220 at 270.930 kb on - strand, within CSW01_15220 at 270.930 kb on - strand, within CSW01_15220 at 270.942 kb on - strand, within CSW01_15220 at 270.992 kb on - strand, within CSW01_15220
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 268,559 + CSW01_15210 0.22 +0.3 268,582 + CSW01_15210 0.24 +1.4 268,590 - CSW01_15210 0.25 -1.5 268,667 - CSW01_15210 0.32 +1.0 268,671 + CSW01_15210 0.33 +1.3 268,788 + CSW01_15210 0.44 +1.7 268,807 - CSW01_15210 0.45 -0.9 268,881 - CSW01_15210 0.52 +1.4 268,936 + CSW01_15210 0.57 +0.9 268,936 + CSW01_15210 0.57 +1.9 268,944 - CSW01_15210 0.58 -0.2 268,986 + CSW01_15210 0.62 -3.3 268,994 - CSW01_15210 0.63 -2.0 268,994 - CSW01_15210 0.63 -0.5 269,028 + CSW01_15210 0.66 -1.9 269,055 - CSW01_15210 0.69 -0.9 269,128 + CSW01_15210 0.75 -0.8 269,391 + +0.1 269,468 - +0.5 269,481 - +0.7 269,486 + -0.5 269,513 - +0.3 269,518 + +0.3 269,622 - CSW01_15215 0.25 +1.8 269,658 - CSW01_15215 0.31 +0.4 269,660 + CSW01_15215 0.32 -0.1 269,675 - CSW01_15215 0.35 -0.0 269,683 - CSW01_15215 0.36 +0.2 269,867 - CSW01_15215 0.71 +0.6 269,875 + CSW01_15215 0.72 +0.4 269,875 + CSW01_15215 0.72 -2.6 269,883 - CSW01_15215 0.74 -0.4 269,930 + CSW01_15215 0.82 +0.9 269,982 - +1.7 269,992 - +0.8 270,112 + +0.5 270,201 - -1.4 270,201 - -1.7 270,203 + +1.3 270,266 - +2.0 270,266 - +2.1 270,292 + -0.2 270,405 - CSW01_15220 0.15 -2.3 270,555 + CSW01_15220 0.26 +2.0 270,563 - CSW01_15220 0.26 -1.0 270,711 - CSW01_15220 0.37 -0.7 270,805 - CSW01_15220 0.43 +0.7 270,807 + CSW01_15220 0.44 +0.1 270,824 + CSW01_15220 0.45 -1.9 270,825 + CSW01_15220 0.45 +0.5 270,841 + CSW01_15220 0.46 +0.4 270,841 + CSW01_15220 0.46 +0.9 270,907 + CSW01_15220 0.51 -1.6 270,915 - CSW01_15220 0.51 -1.3 270,930 - CSW01_15220 0.52 +0.6 270,930 - CSW01_15220 0.52 -2.3 270,942 - CSW01_15220 0.53 -0.6 270,992 - CSW01_15220 0.57 -0.8
Or see this region's nucleotide sequence