Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_10525 and CSW01_10530 are separated by 185 nucleotides CSW01_10530 and CSW01_10535 are separated by 117 nucleotides
CSW01_10525: CSW01_10525 - DUF3410 domain-containing protein, at 2,263,571 to 2,264,734
_10525
CSW01_10530: CSW01_10530 - beta-ketoacyl-[acyl-carrier-protein] synthase I, at 2,264,920 to 2,266,131
_10530
CSW01_10535: CSW01_10535 - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 2,266,249 to 2,268,273
_10535
Position (kb)
2264
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2264.917 kb on + strand at 2264.917 kb on + strand at 2266.230 kb on - strand at 2266.231 kb on + strand at 2266.285 kb on + strand at 2266.366 kb on + strand at 2266.403 kb on - strand at 2266.459 kb on + strand, within CSW01_10535 at 2266.566 kb on + strand, within CSW01_10535 at 2266.591 kb on + strand, within CSW01_10535 at 2266.591 kb on + strand, within CSW01_10535 at 2266.591 kb on + strand, within CSW01_10535 at 2266.591 kb on + strand, within CSW01_10535 at 2266.591 kb on + strand, within CSW01_10535 at 2266.599 kb on - strand, within CSW01_10535 at 2266.599 kb on - strand, within CSW01_10535 at 2266.645 kb on + strand, within CSW01_10535 at 2266.675 kb on + strand, within CSW01_10535 at 2266.728 kb on - strand, within CSW01_10535 at 2266.728 kb on - strand, within CSW01_10535 at 2266.728 kb on - strand, within CSW01_10535 at 2266.753 kb on + strand, within CSW01_10535 at 2266.753 kb on + strand, within CSW01_10535 at 2266.760 kb on + strand, within CSW01_10535 at 2266.760 kb on + strand, within CSW01_10535 at 2266.761 kb on - strand, within CSW01_10535 at 2266.768 kb on - strand, within CSW01_10535 at 2266.838 kb on - strand, within CSW01_10535 at 2266.947 kb on - strand, within CSW01_10535 at 2266.955 kb on - strand, within CSW01_10535 at 2267.042 kb on + strand, within CSW01_10535 at 2267.042 kb on + strand, within CSW01_10535 at 2267.050 kb on - strand, within CSW01_10535 at 2267.079 kb on - strand, within CSW01_10535 at 2267.098 kb on + strand, within CSW01_10535 at 2267.098 kb on + strand, within CSW01_10535 at 2267.099 kb on - strand, within CSW01_10535 at 2267.106 kb on - strand, within CSW01_10535 at 2267.106 kb on - strand, within CSW01_10535
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 2,264,917 + +0.2 2,264,917 + -1.8 2,266,230 - +0.6 2,266,231 + +0.4 2,266,285 + +0.6 2,266,366 + -2.5 2,266,403 - -1.1 2,266,459 + CSW01_10535 0.10 -0.7 2,266,566 + CSW01_10535 0.16 -0.6 2,266,591 + CSW01_10535 0.17 +1.0 2,266,591 + CSW01_10535 0.17 +1.0 2,266,591 + CSW01_10535 0.17 -0.8 2,266,591 + CSW01_10535 0.17 +1.5 2,266,591 + CSW01_10535 0.17 +1.1 2,266,599 - CSW01_10535 0.17 -1.5 2,266,599 - CSW01_10535 0.17 -0.7 2,266,645 + CSW01_10535 0.20 +0.1 2,266,675 + CSW01_10535 0.21 -0.9 2,266,728 - CSW01_10535 0.24 +1.2 2,266,728 - CSW01_10535 0.24 -1.6 2,266,728 - CSW01_10535 0.24 -0.3 2,266,753 + CSW01_10535 0.25 -2.8 2,266,753 + CSW01_10535 0.25 +0.2 2,266,760 + CSW01_10535 0.25 +1.6 2,266,760 + CSW01_10535 0.25 +1.3 2,266,761 - CSW01_10535 0.25 -0.3 2,266,768 - CSW01_10535 0.26 -0.1 2,266,838 - CSW01_10535 0.29 -0.9 2,266,947 - CSW01_10535 0.34 -0.5 2,266,955 - CSW01_10535 0.35 +0.2 2,267,042 + CSW01_10535 0.39 +0.1 2,267,042 + CSW01_10535 0.39 -0.2 2,267,050 - CSW01_10535 0.40 +1.8 2,267,079 - CSW01_10535 0.41 +1.6 2,267,098 + CSW01_10535 0.42 -2.4 2,267,098 + CSW01_10535 0.42 +0.2 2,267,099 - CSW01_10535 0.42 +0.6 2,267,106 - CSW01_10535 0.42 -0.3 2,267,106 - CSW01_10535 0.42 -1.7
Or see this region's nucleotide sequence