Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_09700 and CSW01_09705 are separated by 312 nucleotides CSW01_09705 and CSW01_09710 are separated by 283 nucleotides CSW01_09710 and CSW01_09715 overlap by 49 nucleotides CSW01_09715 and CSW01_09720 are separated by 126 nucleotides
CSW01_09700: CSW01_09700 - HAD-IB family hydrolase, at 2,089,463 to 2,090,125
_09700
CSW01_09705: CSW01_09705 - hypothetical protein, at 2,090,438 to 2,091,733
_09705
CSW01_09710: CSW01_09710 - peptidylprolyl isomerase, at 2,092,017 to 2,092,133
_09710
CSW01_09715: CSW01_09715 - tRNA-Arg, at 2,092,085 to 2,092,161
_09715
CSW01_09720: CSW01_09720 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 2,092,288 to 2,093,223
_09720
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2089.460 kb on + strand at 2089.460 kb on + strand at 2089.460 kb on + strand at 2089.462 kb on - strand at 2089.468 kb on - strand at 2089.468 kb on - strand at 2089.478 kb on - strand at 2089.483 kb on - strand at 2089.530 kb on - strand, within CSW01_09700 at 2089.600 kb on - strand, within CSW01_09700 at 2089.611 kb on + strand, within CSW01_09700 at 2089.611 kb on + strand, within CSW01_09700 at 2089.619 kb on - strand, within CSW01_09700 at 2089.619 kb on - strand, within CSW01_09700 at 2089.631 kb on + strand, within CSW01_09700 at 2089.632 kb on - strand, within CSW01_09700 at 2089.685 kb on - strand, within CSW01_09700 at 2089.803 kb on + strand, within CSW01_09700 at 2089.811 kb on - strand, within CSW01_09700 at 2089.879 kb on - strand, within CSW01_09700 at 2089.911 kb on - strand, within CSW01_09700 at 2089.972 kb on - strand, within CSW01_09700 at 2090.041 kb on - strand, within CSW01_09700 at 2090.055 kb on + strand, within CSW01_09700 at 2090.063 kb on - strand at 2090.075 kb on - strand at 2090.114 kb on - strand at 2090.134 kb on + strand at 2090.209 kb on - strand at 2090.288 kb on + strand at 2090.296 kb on + strand at 2090.296 kb on - strand at 2090.342 kb on - strand at 2090.361 kb on + strand at 2090.374 kb on - strand at 2090.376 kb on + strand at 2090.421 kb on + strand at 2090.441 kb on + strand at 2090.449 kb on - strand at 2090.449 kb on - strand at 2090.449 kb on - strand at 2090.449 kb on - strand at 2090.507 kb on + strand at 2090.507 kb on + strand at 2090.527 kb on + strand at 2090.535 kb on - strand at 2090.669 kb on - strand, within CSW01_09705 at 2090.686 kb on + strand, within CSW01_09705 at 2090.686 kb on + strand, within CSW01_09705 at 2090.686 kb on + strand, within CSW01_09705 at 2090.688 kb on + strand, within CSW01_09705 at 2090.688 kb on + strand, within CSW01_09705 at 2090.688 kb on + strand, within CSW01_09705 at 2090.688 kb on + strand, within CSW01_09705 at 2090.691 kb on + strand, within CSW01_09705 at 2090.694 kb on - strand, within CSW01_09705 at 2090.717 kb on + strand, within CSW01_09705 at 2090.809 kb on + strand, within CSW01_09705 at 2090.831 kb on + strand, within CSW01_09705 at 2090.839 kb on - strand, within CSW01_09705 at 2090.863 kb on - strand, within CSW01_09705 at 2090.863 kb on - strand, within CSW01_09705 at 2090.886 kb on + strand, within CSW01_09705 at 2090.894 kb on - strand, within CSW01_09705 at 2090.919 kb on + strand, within CSW01_09705 at 2091.122 kb on - strand, within CSW01_09705 at 2091.337 kb on + strand, within CSW01_09705 at 2091.345 kb on - strand, within CSW01_09705 at 2091.345 kb on - strand, within CSW01_09705 at 2091.407 kb on + strand, within CSW01_09705 at 2091.461 kb on - strand, within CSW01_09705 at 2091.500 kb on + strand, within CSW01_09705 at 2091.508 kb on - strand, within CSW01_09705 at 2091.553 kb on - strand, within CSW01_09705 at 2091.581 kb on + strand, within CSW01_09705 at 2091.710 kb on + strand at 2091.869 kb on - strand at 2091.882 kb on + strand at 2091.882 kb on + strand at 2091.890 kb on - strand at 2091.893 kb on + strand at 2092.182 kb on - strand at 2092.260 kb on - strand at 2092.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 2,089,460 + -2.1 2,089,460 + +0.6 2,089,460 + +0.2 2,089,462 - -1.9 2,089,468 - -1.0 2,089,468 - +1.2 2,089,478 - -2.2 2,089,483 - -0.5 2,089,530 - CSW01_09700 0.10 +1.4 2,089,600 - CSW01_09700 0.21 -0.2 2,089,611 + CSW01_09700 0.22 -1.1 2,089,611 + CSW01_09700 0.22 +1.9 2,089,619 - CSW01_09700 0.24 +0.5 2,089,619 - CSW01_09700 0.24 +0.2 2,089,631 + CSW01_09700 0.25 -3.7 2,089,632 - CSW01_09700 0.25 -0.4 2,089,685 - CSW01_09700 0.33 -3.2 2,089,803 + CSW01_09700 0.51 -3.9 2,089,811 - CSW01_09700 0.52 -0.1 2,089,879 - CSW01_09700 0.63 +1.5 2,089,911 - CSW01_09700 0.68 -0.5 2,089,972 - CSW01_09700 0.77 +1.9 2,090,041 - CSW01_09700 0.87 -0.4 2,090,055 + CSW01_09700 0.89 +0.4 2,090,063 - +1.5 2,090,075 - +1.1 2,090,114 - +1.1 2,090,134 + +0.8 2,090,209 - -3.2 2,090,288 + -1.2 2,090,296 + +0.9 2,090,296 - -0.6 2,090,342 - +0.3 2,090,361 + -1.5 2,090,374 - +0.4 2,090,376 + -3.1 2,090,421 + -0.3 2,090,441 + +2.0 2,090,449 - -0.9 2,090,449 - -0.0 2,090,449 - -1.5 2,090,449 - -0.0 2,090,507 + +1.1 2,090,507 + -2.3 2,090,527 + -1.7 2,090,535 - -2.4 2,090,669 - CSW01_09705 0.18 -3.2 2,090,686 + CSW01_09705 0.19 -0.1 2,090,686 + CSW01_09705 0.19 -1.2 2,090,686 + CSW01_09705 0.19 -1.6 2,090,688 + CSW01_09705 0.19 -0.8 2,090,688 + CSW01_09705 0.19 -1.3 2,090,688 + CSW01_09705 0.19 +0.3 2,090,688 + CSW01_09705 0.19 +0.4 2,090,691 + CSW01_09705 0.20 +0.2 2,090,694 - CSW01_09705 0.20 +0.6 2,090,717 + CSW01_09705 0.22 +0.7 2,090,809 + CSW01_09705 0.29 -2.7 2,090,831 + CSW01_09705 0.30 -0.6 2,090,839 - CSW01_09705 0.31 -1.9 2,090,863 - CSW01_09705 0.33 -0.9 2,090,863 - CSW01_09705 0.33 +0.2 2,090,886 + CSW01_09705 0.35 -2.0 2,090,894 - CSW01_09705 0.35 -1.5 2,090,919 + CSW01_09705 0.37 +0.7 2,091,122 - CSW01_09705 0.53 +1.1 2,091,337 + CSW01_09705 0.69 +0.7 2,091,345 - CSW01_09705 0.70 -0.2 2,091,345 - CSW01_09705 0.70 -1.1 2,091,407 + CSW01_09705 0.75 +0.3 2,091,461 - CSW01_09705 0.79 +0.8 2,091,500 + CSW01_09705 0.82 -1.1 2,091,508 - CSW01_09705 0.83 -1.1 2,091,553 - CSW01_09705 0.86 +0.3 2,091,581 + CSW01_09705 0.88 +0.3 2,091,710 + -1.0 2,091,869 - +0.8 2,091,882 + -1.1 2,091,882 + +1.2 2,091,890 - +0.6 2,091,893 + -0.1 2,092,182 - -0.3 2,092,260 - -0.6 2,092,341 + +1.2
Or see this region's nucleotide sequence