Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03385 and CSW01_03390 are separated by 24 nucleotides CSW01_03390 and CSW01_03395 are separated by 101 nucleotides CSW01_03395 and CSW01_03400 overlap by 1 nucleotides
CSW01_03385: CSW01_03385 - transcription termination/antitermination protein NusA, at 682,569 to 684,056
_03385
CSW01_03390: CSW01_03390 - translation initiation factor IF-2, at 684,081 to 686,777
_03390
CSW01_03395: CSW01_03395 - ribosome-binding factor A, at 686,879 to 687,277
_03395
CSW01_03400: CSW01_03400 - tRNA pseudouridine(55) synthase TruB, at 687,277 to 688,215
_03400
Position (kb) 684
685
686
687 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 683.974 kb on + strand at 686.814 kb on + strand at 686.836 kb on + strand at 686.836 kb on + strand at 687.280 kb on - strand at 687.311 kb on + strand at 687.374 kb on - strand, within CSW01_03400 at 687.383 kb on - strand, within CSW01_03400 at 687.454 kb on - strand, within CSW01_03400 at 687.531 kb on - strand, within CSW01_03400 at 687.603 kb on - strand, within CSW01_03400 at 687.632 kb on + strand, within CSW01_03400 at 687.709 kb on - strand, within CSW01_03400 at 687.709 kb on - strand, within CSW01_03400 at 687.736 kb on + strand, within CSW01_03400 at 687.736 kb on - strand, within CSW01_03400 at 687.761 kb on - strand, within CSW01_03400 at 687.767 kb on - strand, within CSW01_03400
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 683,974 + +0.1 686,814 + -1.9 686,836 + -2.5 686,836 + +1.4 687,280 - +0.0 687,311 + +0.0 687,374 - CSW01_03400 0.10 +0.7 687,383 - CSW01_03400 0.11 -2.7 687,454 - CSW01_03400 0.19 +0.8 687,531 - CSW01_03400 0.27 -0.1 687,603 - CSW01_03400 0.35 +1.5 687,632 + CSW01_03400 0.38 -0.5 687,709 - CSW01_03400 0.46 -1.7 687,709 - CSW01_03400 0.46 -3.0 687,736 + CSW01_03400 0.49 -0.1 687,736 - CSW01_03400 0.49 +0.3 687,761 - CSW01_03400 0.52 +1.6 687,767 - CSW01_03400 0.52 -1.8
Or see this region's nucleotide sequence