Experiment: ICP1_phage_235.3_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_02535 and CSW01_02540 are separated by 155 nucleotides CSW01_02540 and CSW01_02545 are separated by 187 nucleotides CSW01_02545 and CSW01_02550 are separated by 12 nucleotides
CSW01_02535: CSW01_02535 - phosphoglycerate kinase, at 506,826 to 507,989
_02535
CSW01_02540: CSW01_02540 - class II fructose-bisphosphate aldolase, at 508,145 to 509,221
_02540
CSW01_02545: CSW01_02545 - hypothetical protein, at 509,409 to 509,504
_02545
CSW01_02550: CSW01_02550 - mechanosensitive ion channel protein, at 509,517 to 510,380
_02550
Position (kb) 508
509
510 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 507.965 kb on + strand at 509.261 kb on - strand at 509.292 kb on - strand at 509.400 kb on + strand at 509.409 kb on - strand at 509.568 kb on - strand at 509.578 kb on - strand at 509.612 kb on + strand, within CSW01_02550 at 509.645 kb on - strand, within CSW01_02550 at 509.674 kb on - strand, within CSW01_02550 at 509.725 kb on - strand, within CSW01_02550 at 509.727 kb on + strand, within CSW01_02550 at 509.734 kb on + strand, within CSW01_02550 at 509.735 kb on - strand, within CSW01_02550 at 509.735 kb on - strand, within CSW01_02550 at 509.746 kb on + strand, within CSW01_02550 at 509.746 kb on + strand, within CSW01_02550 at 509.754 kb on - strand, within CSW01_02550 at 509.773 kb on - strand, within CSW01_02550 at 509.805 kb on - strand, within CSW01_02550 at 509.870 kb on - strand, within CSW01_02550 at 509.994 kb on + strand, within CSW01_02550 at 509.994 kb on + strand, within CSW01_02550 at 510.118 kb on + strand, within CSW01_02550
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep2 remove 507,965 + -1.0 509,261 - -2.9 509,292 - +1.2 509,400 + +0.2 509,409 - +0.3 509,568 - -1.1 509,578 - -0.1 509,612 + CSW01_02550 0.11 -0.7 509,645 - CSW01_02550 0.15 -2.8 509,674 - CSW01_02550 0.18 -1.6 509,725 - CSW01_02550 0.24 +0.8 509,727 + CSW01_02550 0.24 -2.4 509,734 + CSW01_02550 0.25 -2.3 509,735 - CSW01_02550 0.25 -0.9 509,735 - CSW01_02550 0.25 -1.3 509,746 + CSW01_02550 0.27 -3.1 509,746 + CSW01_02550 0.27 -1.0 509,754 - CSW01_02550 0.27 -1.5 509,773 - CSW01_02550 0.30 +1.7 509,805 - CSW01_02550 0.33 -3.1 509,870 - CSW01_02550 0.41 -1.6 509,994 + CSW01_02550 0.55 -2.9 509,994 + CSW01_02550 0.55 +0.3 510,118 + CSW01_02550 0.70 +0.6
Or see this region's nucleotide sequence