Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1942 and Ac3H11_1943 overlap by 4 nucleotides Ac3H11_1943 and Ac3H11_1944 are separated by 187 nucleotides Ac3H11_1944 and Ac3H11_1945 are separated by 66 nucleotides Ac3H11_1945 and Ac3H11_1946 are separated by 128 nucleotides
Ac3H11_1942 - Phenylacetic acid degradation protein paaI, at 157,595 to 158,053
_1942
Ac3H11_1943 - Cu(I)-responsive transcriptional regulator, at 158,050 to 158,505
_1943
Ac3H11_1944 - COG1664: Integral membrane protein CcmA involved in cell shape determination, at 158,693 to 159,277
_1944
Ac3H11_1945 - Acyl-CoA hydrolase (EC 3.1.2.20), at 159,344 to 159,766
_1945
Ac3H11_1946 - Lipid A export ATP-binding/permease protein MsbA, at 159,895 to 161,742
_1946
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2 at 157.860 kb on + strand, within Ac3H11_1942 at 157.861 kb on - strand, within Ac3H11_1942 at 157.861 kb on - strand, within Ac3H11_1942 at 157.946 kb on - strand, within Ac3H11_1942 at 158.330 kb on - strand, within Ac3H11_1943 at 158.448 kb on - strand, within Ac3H11_1943 at 158.497 kb on + strand at 158.497 kb on + strand at 158.517 kb on + strand at 158.517 kb on + strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.518 kb on - strand at 158.665 kb on + strand at 158.666 kb on - strand at 158.666 kb on - strand at 159.083 kb on + strand, within Ac3H11_1944 at 159.284 kb on + strand at 159.285 kb on - strand at 159.391 kb on + strand, within Ac3H11_1945 at 159.391 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.405 kb on + strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.406 kb on - strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.435 kb on + strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.436 kb on - strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.465 kb on + strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.466 kb on - strand, within Ac3H11_1945 at 159.603 kb on + strand, within Ac3H11_1945 at 159.603 kb on + strand, within Ac3H11_1945 at 159.604 kb on - strand, within Ac3H11_1945 at 159.694 kb on + strand, within Ac3H11_1945 at 159.694 kb on + strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 159.695 kb on - strand, within Ac3H11_1945 at 160.024 kb on + strand at 160.024 kb on + strand at 160.025 kb on - strand at 160.025 kb on - strand at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.183 kb on + strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946 at 160.184 kb on - strand, within Ac3H11_1946
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 157,860 + Ac3H11_1942 0.58 +2.1 157,861 - Ac3H11_1942 0.58 -0.5 157,861 - Ac3H11_1942 0.58 -0.5 157,946 - Ac3H11_1942 0.76 +0.3 158,330 - Ac3H11_1943 0.61 +2.3 158,448 - Ac3H11_1943 0.87 -0.3 158,497 + -0.6 158,497 + -0.9 158,517 + -0.2 158,517 + -0.6 158,518 - -0.5 158,518 - +0.3 158,518 - +0.3 158,518 - +1.1 158,518 - -0.1 158,665 + +0.5 158,666 - +0.4 158,666 - -0.8 159,083 + Ac3H11_1944 0.67 +1.3 159,284 + +1.4 159,285 - +0.6 159,391 + Ac3H11_1945 0.11 -0.4 159,391 + Ac3H11_1945 0.11 +0.6 159,405 + Ac3H11_1945 0.14 -0.8 159,405 + Ac3H11_1945 0.14 +0.4 159,405 + Ac3H11_1945 0.14 -0.3 159,405 + Ac3H11_1945 0.14 -1.0 159,406 - Ac3H11_1945 0.15 -0.7 159,406 - Ac3H11_1945 0.15 -1.2 159,406 - Ac3H11_1945 0.15 +0.8 159,435 + Ac3H11_1945 0.22 +0.5 159,435 + Ac3H11_1945 0.22 -0.7 159,435 + Ac3H11_1945 0.22 +1.0 159,435 + Ac3H11_1945 0.22 -0.4 159,435 + Ac3H11_1945 0.22 +1.8 159,435 + Ac3H11_1945 0.22 +0.9 159,435 + Ac3H11_1945 0.22 -0.1 159,435 + Ac3H11_1945 0.22 -0.9 159,436 - Ac3H11_1945 0.22 -0.1 159,436 - Ac3H11_1945 0.22 -0.7 159,436 - Ac3H11_1945 0.22 -0.1 159,436 - Ac3H11_1945 0.22 +1.0 159,436 - Ac3H11_1945 0.22 -0.2 159,465 + Ac3H11_1945 0.29 -0.3 159,465 + Ac3H11_1945 0.29 +2.4 159,465 + Ac3H11_1945 0.29 -0.8 159,465 + Ac3H11_1945 0.29 +0.4 159,465 + Ac3H11_1945 0.29 +0.6 159,465 + Ac3H11_1945 0.29 +0.6 159,465 + Ac3H11_1945 0.29 +1.5 159,465 + Ac3H11_1945 0.29 -0.4 159,466 - Ac3H11_1945 0.29 +1.6 159,466 - Ac3H11_1945 0.29 -0.4 159,466 - Ac3H11_1945 0.29 -0.9 159,466 - Ac3H11_1945 0.29 -0.4 159,603 + Ac3H11_1945 0.61 -0.6 159,603 + Ac3H11_1945 0.61 -0.5 159,604 - Ac3H11_1945 0.61 -2.0 159,694 + Ac3H11_1945 0.83 -0.7 159,694 + Ac3H11_1945 0.83 -0.8 159,695 - Ac3H11_1945 0.83 -0.6 159,695 - Ac3H11_1945 0.83 -1.8 159,695 - Ac3H11_1945 0.83 -0.9 159,695 - Ac3H11_1945 0.83 -0.7 159,695 - Ac3H11_1945 0.83 -1.1 160,024 + -0.5 160,024 + -0.0 160,025 - +0.3 160,025 - -0.1 160,183 + Ac3H11_1946 0.16 -0.1 160,183 + Ac3H11_1946 0.16 -0.7 160,183 + Ac3H11_1946 0.16 +0.9 160,183 + Ac3H11_1946 0.16 +0.2 160,184 - Ac3H11_1946 0.16 -2.0 160,184 - Ac3H11_1946 0.16 -0.2 160,184 - Ac3H11_1946 0.16 -0.3
Or see this region's nucleotide sequence