Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25845 and RR42_RS25850 are separated by 494 nucleotides RR42_RS25850 and RR42_RS25855 are separated by 23 nucleotides
RR42_RS25845: RR42_RS25845 - isocitrate lyase, at 1,137,513 to 1,139,093
_RS25845
RR42_RS25850: RR42_RS25850 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,139,588 to 1,140,958
_RS25850
RR42_RS25855: RR42_RS25855 - sodium:dicarboxylate symporter, at 1,140,982 to 1,142,388
_RS25855
Position (kb)
1139
1140
1141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1138.635 kb on + strand, within RR42_RS25845 at 1138.635 kb on + strand, within RR42_RS25845 at 1138.636 kb on - strand, within RR42_RS25845 at 1138.636 kb on - strand, within RR42_RS25845 at 1138.686 kb on + strand, within RR42_RS25845 at 1138.686 kb on + strand, within RR42_RS25845 at 1138.686 kb on + strand, within RR42_RS25845 at 1138.686 kb on + strand, within RR42_RS25845 at 1138.687 kb on - strand, within RR42_RS25845 at 1138.932 kb on + strand, within RR42_RS25845 at 1138.932 kb on + strand, within RR42_RS25845 at 1138.933 kb on - strand, within RR42_RS25845 at 1138.933 kb on - strand, within RR42_RS25845 at 1139.031 kb on + strand at 1139.031 kb on + strand at 1139.067 kb on - strand at 1139.094 kb on + strand at 1139.100 kb on + strand at 1139.100 kb on + strand at 1139.101 kb on - strand at 1139.101 kb on - strand at 1139.101 kb on - strand at 1139.101 kb on - strand at 1139.101 kb on - strand at 1139.104 kb on - strand at 1139.104 kb on - strand at 1139.195 kb on + strand at 1139.196 kb on - strand at 1139.371 kb on + strand at 1139.372 kb on - strand at 1139.372 kb on - strand at 1139.573 kb on - strand at 1139.587 kb on + strand at 1139.587 kb on + strand at 1139.615 kb on - strand at 1139.705 kb on + strand at 1139.705 kb on + strand at 1139.706 kb on - strand at 1139.821 kb on + strand, within RR42_RS25850 at 1139.821 kb on + strand, within RR42_RS25850 at 1139.821 kb on + strand, within RR42_RS25850 at 1139.821 kb on + strand, within RR42_RS25850 at 1139.822 kb on - strand, within RR42_RS25850 at 1139.822 kb on - strand, within RR42_RS25850 at 1139.822 kb on - strand, within RR42_RS25850 at 1139.907 kb on - strand, within RR42_RS25850 at 1139.907 kb on - strand, within RR42_RS25850 at 1139.976 kb on + strand, within RR42_RS25850 at 1139.977 kb on - strand, within RR42_RS25850 at 1140.102 kb on - strand, within RR42_RS25850 at 1140.109 kb on - strand, within RR42_RS25850 at 1140.899 kb on + strand at 1140.899 kb on + strand at 1140.899 kb on + strand at 1140.900 kb on - strand at 1140.956 kb on + strand at 1140.956 kb on + strand at 1140.957 kb on - strand at 1140.957 kb on - strand at 1141.265 kb on - strand, within RR42_RS25855 at 1141.349 kb on + strand, within RR42_RS25855 at 1141.568 kb on + strand, within RR42_RS25855 at 1141.569 kb on - strand, within RR42_RS25855 at 1141.581 kb on + strand, within RR42_RS25855 at 1141.628 kb on + strand, within RR42_RS25855 at 1141.629 kb on - strand, within RR42_RS25855 at 1141.634 kb on - strand, within RR42_RS25855 at 1141.697 kb on - strand, within RR42_RS25855 at 1141.780 kb on + strand, within RR42_RS25855 at 1141.781 kb on - strand, within RR42_RS25855 at 1141.865 kb on + strand, within RR42_RS25855 at 1141.866 kb on - strand, within RR42_RS25855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 1,138,635 + RR42_RS25845 0.71 -0.1 1,138,635 + RR42_RS25845 0.71 +1.0 1,138,636 - RR42_RS25845 0.71 +0.9 1,138,636 - RR42_RS25845 0.71 +0.6 1,138,686 + RR42_RS25845 0.74 +0.9 1,138,686 + RR42_RS25845 0.74 +1.3 1,138,686 + RR42_RS25845 0.74 -0.7 1,138,686 + RR42_RS25845 0.74 +0.2 1,138,687 - RR42_RS25845 0.74 -0.5 1,138,932 + RR42_RS25845 0.90 -0.5 1,138,932 + RR42_RS25845 0.90 +0.6 1,138,933 - RR42_RS25845 0.90 +0.2 1,138,933 - RR42_RS25845 0.90 -1.0 1,139,031 + +0.3 1,139,031 + -2.5 1,139,067 - +0.7 1,139,094 + +0.0 1,139,100 + -0.6 1,139,100 + +0.2 1,139,101 - -1.7 1,139,101 - +0.5 1,139,101 - -0.6 1,139,101 - -0.3 1,139,101 - -0.7 1,139,104 - -0.1 1,139,104 - +0.1 1,139,195 + +0.2 1,139,196 - -0.0 1,139,371 + +0.8 1,139,372 - +0.1 1,139,372 - +0.2 1,139,573 - +0.1 1,139,587 + +0.6 1,139,587 + -0.7 1,139,615 - +0.7 1,139,705 + -1.0 1,139,705 + +0.1 1,139,706 - +0.1 1,139,821 + RR42_RS25850 0.17 +0.2 1,139,821 + RR42_RS25850 0.17 -0.7 1,139,821 + RR42_RS25850 0.17 -0.0 1,139,821 + RR42_RS25850 0.17 +0.0 1,139,822 - RR42_RS25850 0.17 +0.0 1,139,822 - RR42_RS25850 0.17 -0.9 1,139,822 - RR42_RS25850 0.17 +0.2 1,139,907 - RR42_RS25850 0.23 -0.6 1,139,907 - RR42_RS25850 0.23 -0.1 1,139,976 + RR42_RS25850 0.28 -1.1 1,139,977 - RR42_RS25850 0.28 -0.0 1,140,102 - RR42_RS25850 0.37 -0.2 1,140,109 - RR42_RS25850 0.38 +0.1 1,140,899 + -0.2 1,140,899 + +0.1 1,140,899 + +1.0 1,140,900 - -0.2 1,140,956 + +0.2 1,140,956 + +0.4 1,140,957 - -0.3 1,140,957 - +0.1 1,141,265 - RR42_RS25855 0.20 +2.1 1,141,349 + RR42_RS25855 0.26 +0.3 1,141,568 + RR42_RS25855 0.42 -0.6 1,141,569 - RR42_RS25855 0.42 +0.6 1,141,581 + RR42_RS25855 0.43 +0.1 1,141,628 + RR42_RS25855 0.46 +1.0 1,141,629 - RR42_RS25855 0.46 -0.5 1,141,634 - RR42_RS25855 0.46 +0.1 1,141,697 - RR42_RS25855 0.51 -0.5 1,141,780 + RR42_RS25855 0.57 -0.3 1,141,781 - RR42_RS25855 0.57 -0.5 1,141,865 + RR42_RS25855 0.63 -3.6 1,141,866 - RR42_RS25855 0.63 +2.2
Or see this region's nucleotide sequence