Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21700 and RR42_RS21705 are separated by 284 nucleotides RR42_RS21705 and RR42_RS21710 are separated by 81 nucleotides
RR42_RS21700: RR42_RS21700 - membrane protein, at 195,564 to 197,135
_RS21700
RR42_RS21705: RR42_RS21705 - alcohol dehydrogenase, at 197,420 to 198,385
_RS21705
RR42_RS21710: RR42_RS21710 - haloacid dehalogenase, at 198,467 to 199,174
_RS21710
Position (kb)
197
198
199 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 196.631 kb on - strand, within RR42_RS21700 at 196.631 kb on - strand, within RR42_RS21700 at 196.631 kb on - strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.639 kb on + strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.640 kb on - strand, within RR42_RS21700 at 196.760 kb on - strand, within RR42_RS21700 at 196.867 kb on + strand, within RR42_RS21700 at 196.868 kb on - strand, within RR42_RS21700 at 196.868 kb on - strand, within RR42_RS21700 at 196.963 kb on + strand, within RR42_RS21700 at 196.963 kb on + strand, within RR42_RS21700 at 196.964 kb on - strand, within RR42_RS21700 at 197.300 kb on + strand at 197.301 kb on - strand at 197.301 kb on - strand at 197.311 kb on - strand at 197.356 kb on + strand at 197.356 kb on + strand at 197.356 kb on + strand at 197.357 kb on - strand at 197.589 kb on - strand, within RR42_RS21705 at 197.738 kb on + strand, within RR42_RS21705 at 197.738 kb on + strand, within RR42_RS21705 at 197.739 kb on - strand, within RR42_RS21705 at 197.741 kb on - strand, within RR42_RS21705 at 197.787 kb on - strand, within RR42_RS21705 at 197.798 kb on + strand, within RR42_RS21705 at 197.799 kb on - strand, within RR42_RS21705 at 197.907 kb on - strand, within RR42_RS21705 at 197.987 kb on + strand, within RR42_RS21705 at 197.987 kb on + strand, within RR42_RS21705 at 197.988 kb on - strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.996 kb on + strand, within RR42_RS21705 at 197.997 kb on - strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.065 kb on + strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.066 kb on - strand, within RR42_RS21705 at 198.069 kb on - strand, within RR42_RS21705 at 198.101 kb on + strand, within RR42_RS21705 at 198.101 kb on + strand, within RR42_RS21705 at 198.237 kb on + strand, within RR42_RS21705 at 198.237 kb on + strand, within RR42_RS21705 at 198.407 kb on + strand at 198.407 kb on + strand at 198.523 kb on + strand at 198.523 kb on + strand at 198.879 kb on - strand, within RR42_RS21710 at 198.879 kb on - strand, within RR42_RS21710 at 198.950 kb on + strand, within RR42_RS21710 at 198.950 kb on + strand, within RR42_RS21710 at 198.950 kb on + strand, within RR42_RS21710 at 198.951 kb on - strand, within RR42_RS21710 at 199.058 kb on + strand, within RR42_RS21710 at 199.058 kb on + strand, within RR42_RS21710 at 199.059 kb on - strand, within RR42_RS21710 at 199.059 kb on - strand, within RR42_RS21710 at 199.139 kb on - strand at 199.139 kb on - strand at 199.223 kb on + strand at 199.329 kb on + strand at 199.334 kb on + strand at 199.335 kb on - strand at 199.335 kb on - strand at 199.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 196,631 - RR42_RS21700 0.68 +0.3 196,631 - RR42_RS21700 0.68 +0.2 196,631 - RR42_RS21700 0.68 +1.3 196,639 + RR42_RS21700 0.68 -0.4 196,639 + RR42_RS21700 0.68 +0.2 196,639 + RR42_RS21700 0.68 +0.4 196,639 + RR42_RS21700 0.68 +1.1 196,639 + RR42_RS21700 0.68 -0.3 196,639 + RR42_RS21700 0.68 -1.7 196,640 - RR42_RS21700 0.68 +0.0 196,640 - RR42_RS21700 0.68 -0.0 196,640 - RR42_RS21700 0.68 +0.2 196,640 - RR42_RS21700 0.68 -0.9 196,760 - RR42_RS21700 0.76 +1.1 196,867 + RR42_RS21700 0.83 +0.1 196,868 - RR42_RS21700 0.83 -0.1 196,868 - RR42_RS21700 0.83 +0.2 196,963 + RR42_RS21700 0.89 +0.1 196,963 + RR42_RS21700 0.89 +0.6 196,964 - RR42_RS21700 0.89 -1.1 197,300 + +0.6 197,301 - +0.4 197,301 - +0.6 197,311 - +1.1 197,356 + +0.3 197,356 + -0.3 197,356 + -0.1 197,357 - +0.1 197,589 - RR42_RS21705 0.17 +0.2 197,738 + RR42_RS21705 0.33 +0.5 197,738 + RR42_RS21705 0.33 -0.9 197,739 - RR42_RS21705 0.33 +0.6 197,741 - RR42_RS21705 0.33 +1.5 197,787 - RR42_RS21705 0.38 +1.1 197,798 + RR42_RS21705 0.39 +1.6 197,799 - RR42_RS21705 0.39 +0.6 197,907 - RR42_RS21705 0.50 +0.1 197,987 + RR42_RS21705 0.59 +0.6 197,987 + RR42_RS21705 0.59 -0.5 197,988 - RR42_RS21705 0.59 +0.1 197,996 + RR42_RS21705 0.60 +0.8 197,996 + RR42_RS21705 0.60 +1.7 197,996 + RR42_RS21705 0.60 +0.8 197,997 - RR42_RS21705 0.60 +0.4 198,065 + RR42_RS21705 0.67 +0.3 198,065 + RR42_RS21705 0.67 +0.4 198,065 + RR42_RS21705 0.67 -0.4 198,066 - RR42_RS21705 0.67 +0.1 198,066 - RR42_RS21705 0.67 -0.2 198,066 - RR42_RS21705 0.67 +0.2 198,066 - RR42_RS21705 0.67 -2.8 198,069 - RR42_RS21705 0.67 +0.9 198,101 + RR42_RS21705 0.70 -2.1 198,101 + RR42_RS21705 0.70 -0.4 198,237 + RR42_RS21705 0.85 -0.6 198,237 + RR42_RS21705 0.85 +0.7 198,407 + +0.5 198,407 + +0.3 198,523 + -0.5 198,523 + +1.2 198,879 - RR42_RS21710 0.58 +0.7 198,879 - RR42_RS21710 0.58 -1.5 198,950 + RR42_RS21710 0.68 +0.0 198,950 + RR42_RS21710 0.68 +0.2 198,950 + RR42_RS21710 0.68 -0.1 198,951 - RR42_RS21710 0.68 +0.2 199,058 + RR42_RS21710 0.83 +0.6 199,058 + RR42_RS21710 0.83 -0.1 199,059 - RR42_RS21710 0.84 +0.1 199,059 - RR42_RS21710 0.84 +0.2 199,139 - +1.2 199,139 - +0.1 199,223 + -0.6 199,329 + -0.3 199,334 + -0.2 199,335 - -1.3 199,335 - +0.1 199,335 - -0.4
Or see this region's nucleotide sequence