Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14740 and RR42_RS14745 overlap by 4 nucleotides RR42_RS14745 and RR42_RS14750 are separated by 195 nucleotides RR42_RS14750 and RR42_RS14755 are separated by 48 nucleotides RR42_RS14755 and RR42_RS14760 are separated by 110 nucleotides
RR42_RS14740: RR42_RS14740 - thymidylate synthase, at 3,177,476 to 3,178,270
_RS14740
RR42_RS14745: RR42_RS14745 - diacylglycerol kinase, at 3,178,267 to 3,178,767
_RS14745
RR42_RS14750: RR42_RS14750 - peptidase PMbA, at 3,178,963 to 3,180,333
_RS14750
RR42_RS14755: RR42_RS14755 - hypothetical protein, at 3,180,382 to 3,181,008
_RS14755
RR42_RS14760: RR42_RS14760 - molybdenum cofactor biosynthesis protein MogA, at 3,181,119 to 3,181,709
_RS14760
Position (kb)
3178
3179
3180
3181 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3178.784 kb on + strand at 3178.784 kb on + strand at 3178.964 kb on + strand at 3178.964 kb on + strand at 3178.964 kb on + strand at 3179.136 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.172 kb on - strand, within RR42_RS14750 at 3179.336 kb on + strand, within RR42_RS14750 at 3179.336 kb on + strand, within RR42_RS14750 at 3179.337 kb on - strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.351 kb on + strand, within RR42_RS14750 at 3179.359 kb on + strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.360 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.467 kb on - strand, within RR42_RS14750 at 3179.502 kb on - strand, within RR42_RS14750 at 3179.657 kb on + strand, within RR42_RS14750 at 3179.787 kb on - strand, within RR42_RS14750 at 3179.848 kb on - strand, within RR42_RS14750 at 3180.042 kb on - strand, within RR42_RS14750 at 3180.042 kb on - strand, within RR42_RS14750 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.137 kb on - strand, within RR42_RS14750 at 3180.295 kb on - strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.296 kb on + strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.297 kb on - strand at 3180.370 kb on + strand at 3180.370 kb on + strand at 3180.371 kb on - strand at 3180.371 kb on - strand at 3180.378 kb on + strand at 3180.508 kb on - strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.655 kb on + strand, within RR42_RS14755 at 3180.656 kb on - strand, within RR42_RS14755 at 3180.934 kb on - strand, within RR42_RS14755 at 3181.123 kb on + strand at 3181.124 kb on - strand at 3181.164 kb on + strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.165 kb on - strand at 3181.214 kb on + strand, within RR42_RS14760 at 3181.215 kb on - strand, within RR42_RS14760
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 3,178,784 + -0.1 3,178,784 + -1.4 3,178,964 + -0.0 3,178,964 + -1.2 3,178,964 + +0.1 3,179,136 - RR42_RS14750 0.13 -0.7 3,179,172 - RR42_RS14750 0.15 +2.4 3,179,172 - RR42_RS14750 0.15 +0.6 3,179,172 - RR42_RS14750 0.15 -1.5 3,179,172 - RR42_RS14750 0.15 -0.1 3,179,172 - RR42_RS14750 0.15 +1.4 3,179,336 + RR42_RS14750 0.27 -1.4 3,179,336 + RR42_RS14750 0.27 +0.1 3,179,337 - RR42_RS14750 0.27 +0.2 3,179,351 + RR42_RS14750 0.28 +0.4 3,179,351 + RR42_RS14750 0.28 -0.6 3,179,351 + RR42_RS14750 0.28 -0.2 3,179,359 + RR42_RS14750 0.29 -0.1 3,179,360 - RR42_RS14750 0.29 -0.3 3,179,360 - RR42_RS14750 0.29 +1.6 3,179,360 - RR42_RS14750 0.29 -2.0 3,179,467 - RR42_RS14750 0.37 +0.1 3,179,467 - RR42_RS14750 0.37 -0.3 3,179,467 - RR42_RS14750 0.37 +0.0 3,179,467 - RR42_RS14750 0.37 -0.0 3,179,502 - RR42_RS14750 0.39 -0.0 3,179,657 + RR42_RS14750 0.51 +0.2 3,179,787 - RR42_RS14750 0.60 -0.2 3,179,848 - RR42_RS14750 0.65 +0.5 3,180,042 - RR42_RS14750 0.79 +0.1 3,180,042 - RR42_RS14750 0.79 +0.5 3,180,137 - RR42_RS14750 0.86 -1.0 3,180,137 - RR42_RS14750 0.86 +0.4 3,180,295 - -0.2 3,180,296 + -0.4 3,180,296 + -1.4 3,180,296 + -0.0 3,180,296 + -0.5 3,180,297 - -0.1 3,180,297 - +0.7 3,180,297 - +0.4 3,180,370 + +0.4 3,180,370 + -0.0 3,180,371 - +0.3 3,180,371 - -0.1 3,180,378 + +0.5 3,180,508 - RR42_RS14755 0.20 -0.4 3,180,655 + RR42_RS14755 0.44 -1.7 3,180,655 + RR42_RS14755 0.44 +0.3 3,180,655 + RR42_RS14755 0.44 -3.1 3,180,655 + RR42_RS14755 0.44 -0.5 3,180,656 - RR42_RS14755 0.44 -2.3 3,180,934 - RR42_RS14755 0.88 -0.6 3,181,123 + -2.9 3,181,124 - -2.0 3,181,164 + -1.1 3,181,165 - -0.0 3,181,165 - -0.6 3,181,165 - -1.9 3,181,214 + RR42_RS14760 0.16 -1.0 3,181,215 - RR42_RS14760 0.16 -1.7
Or see this region's nucleotide sequence