Experiment: R2A with Chlorite 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11265 and RR42_RS11270 are separated by 110 nucleotides RR42_RS11270 and RR42_RS11275 are separated by 80 nucleotides
RR42_RS11265: RR42_RS11265 - methylcitrate synthase, at 2,408,143 to 2,409,300
_RS11265
RR42_RS11270: RR42_RS11270 - aconitate hydratase, at 2,409,411 to 2,412,008
_RS11270
RR42_RS11275: RR42_RS11275 - 3-methylitaconate isomerase, at 2,412,089 to 2,413,279
_RS11275
Position (kb)
2409
2410
2411
2412
2413 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2409.298 kb on + strand at 2409.298 kb on + strand at 2409.298 kb on + strand at 2409.299 kb on - strand at 2409.299 kb on - strand at 2409.351 kb on + strand at 2409.501 kb on + strand at 2409.502 kb on - strand at 2409.502 kb on - strand at 2409.502 kb on - strand at 2410.326 kb on + strand at 2410.327 kb on - strand, within RR42_RS11270 at 2410.350 kb on + strand, within RR42_RS11270 at 2410.350 kb on + strand, within RR42_RS11270 at 2410.350 kb on + strand, within RR42_RS11270 at 2410.351 kb on - strand, within RR42_RS11270 at 2410.351 kb on - strand, within RR42_RS11270 at 2410.818 kb on + strand, within RR42_RS11270 at 2410.818 kb on + strand, within RR42_RS11270 at 2410.818 kb on + strand, within RR42_RS11270 at 2410.819 kb on - strand, within RR42_RS11270 at 2410.897 kb on - strand, within RR42_RS11270 at 2410.995 kb on + strand, within RR42_RS11270 at 2411.155 kb on - strand, within RR42_RS11270 at 2411.208 kb on + strand, within RR42_RS11270 at 2411.233 kb on - strand, within RR42_RS11270 at 2411.233 kb on - strand, within RR42_RS11270 at 2411.383 kb on - strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.589 kb on + strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.590 kb on - strand, within RR42_RS11270 at 2411.817 kb on + strand at 2411.940 kb on + strand at 2411.941 kb on - strand at 2411.941 kb on - strand at 2411.941 kb on - strand at 2412.006 kb on + strand at 2412.049 kb on - strand at 2412.058 kb on + strand at 2412.059 kb on - strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.125 kb on + strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.126 kb on - strand at 2412.249 kb on - strand, within RR42_RS11275 at 2412.534 kb on + strand, within RR42_RS11275 at 2412.534 kb on + strand, within RR42_RS11275 at 2412.535 kb on - strand, within RR42_RS11275 at 2412.535 kb on - strand, within RR42_RS11275 at 2412.886 kb on + strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.887 kb on - strand, within RR42_RS11275 at 2412.950 kb on + strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275 at 2412.951 kb on - strand, within RR42_RS11275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chlorite 4 mM remove 2,409,298 + +0.2 2,409,298 + +0.1 2,409,298 + +0.2 2,409,299 - -0.4 2,409,299 - -0.9 2,409,351 + +1.2 2,409,501 + -1.2 2,409,502 - -0.3 2,409,502 - -1.2 2,409,502 - +1.0 2,410,326 + +2.6 2,410,327 - RR42_RS11270 0.35 +1.1 2,410,350 + RR42_RS11270 0.36 +0.1 2,410,350 + RR42_RS11270 0.36 +0.5 2,410,350 + RR42_RS11270 0.36 +0.8 2,410,351 - RR42_RS11270 0.36 +0.4 2,410,351 - RR42_RS11270 0.36 +0.3 2,410,818 + RR42_RS11270 0.54 +0.7 2,410,818 + RR42_RS11270 0.54 +0.1 2,410,818 + RR42_RS11270 0.54 +1.4 2,410,819 - RR42_RS11270 0.54 -0.0 2,410,897 - RR42_RS11270 0.57 +1.5 2,410,995 + RR42_RS11270 0.61 -1.2 2,411,155 - RR42_RS11270 0.67 +0.6 2,411,208 + RR42_RS11270 0.69 +1.2 2,411,233 - RR42_RS11270 0.70 -0.1 2,411,233 - RR42_RS11270 0.70 +0.7 2,411,383 - RR42_RS11270 0.76 +0.9 2,411,589 + RR42_RS11270 0.84 +1.5 2,411,589 + RR42_RS11270 0.84 +0.9 2,411,589 + RR42_RS11270 0.84 +0.1 2,411,589 + RR42_RS11270 0.84 +0.5 2,411,589 + RR42_RS11270 0.84 +0.0 2,411,589 + RR42_RS11270 0.84 +0.5 2,411,590 - RR42_RS11270 0.84 +1.5 2,411,590 - RR42_RS11270 0.84 -0.4 2,411,590 - RR42_RS11270 0.84 +0.3 2,411,817 + -0.1 2,411,940 + -0.2 2,411,941 - +0.2 2,411,941 - +0.1 2,411,941 - +0.7 2,412,006 + -0.5 2,412,049 - +0.1 2,412,058 + -0.1 2,412,059 - +0.0 2,412,125 + +0.5 2,412,125 + -1.6 2,412,125 + +0.6 2,412,125 + +0.7 2,412,126 - +0.2 2,412,126 - +0.6 2,412,126 - +0.5 2,412,126 - +0.4 2,412,126 - -1.3 2,412,126 - +0.3 2,412,249 - RR42_RS11275 0.13 -3.0 2,412,534 + RR42_RS11275 0.37 +0.6 2,412,534 + RR42_RS11275 0.37 -0.3 2,412,535 - RR42_RS11275 0.37 -0.1 2,412,535 - RR42_RS11275 0.37 +1.5 2,412,886 + RR42_RS11275 0.67 +0.3 2,412,887 - RR42_RS11275 0.67 +1.3 2,412,887 - RR42_RS11275 0.67 +1.6 2,412,950 + RR42_RS11275 0.72 +0.8 2,412,951 - RR42_RS11275 0.72 -0.5 2,412,951 - RR42_RS11275 0.72 +0.6 2,412,951 - RR42_RS11275 0.72 -0.1
Or see this region's nucleotide sequence